BBa_K1442115 1 BBa_K1442115 B2 3 2014-10-15T11:00:00Z 2015-05-08T01:10:27Z Cytomegalovirus RNA3 Each unique RNA dependent RNA polymerase (RdRP) initiates de novo replication of a RNA strand by interacting with RdRP-specific RNA sequences, henceforth called RdRP/RNA promoters. The RdRP chosen for our project is taken from the Hepatitis C virus (HCV). The B2 RNA promoter was identified as such by Heinz, Kao (2000) , along with a number of other sequences derived from the Brome Mosaic Virus (BMV). All of them possess a few common characteristics: an initiation cytidylate at the 3??? end, where the replication starts; and a stable secondary structure ??? single stranded tail and a stem of various length. Replication stops at the 5??? end. Please refer to Section: RdRP-directed Replication for further details. This is derived from the 3??? end of the Cytomegalovirus RNA3 minus strand. It contains a non-templated guanylate at the 3??? end. These are genomic plus-strand RNAs that can direct BMV minus-strand initiation. This has a 3??? single stranded tail which is required for efficient RNA synthesis. false false _1820_ 0 20675 9 Not in stock true Gel electrophoresis showing replication by HCV RdRP of a number of potential RNA promoters. The bands show the amount of replicated product and its approximate length, and quantify the tendency promoters to produce strands of wrong size. C2 (band 8) shows considerable efficiency in directing replication of the full template, although an amount of incorrect products were also present. false Iva Burova annotation2420903 1 RNA Promoter range2420903 1 7 33 annotation2420832 1 Ribozyme range2420832 1 34 105 BBa_K1442115_sequence 1 cttaagggattgaacctcgttccgtggtttacgggccggcatggtcccagcctcctcgctggcgccggctgggcaacaccattgcactccggtggcgaatgggaccgtacg igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z