BBa_K1442115
1
BBa_K1442115
B2 3
2014-10-15T11:00:00Z
2015-05-08T01:10:27Z
Cytomegalovirus RNA3
Each unique RNA dependent RNA polymerase (RdRP) initiates de novo replication of a RNA strand by interacting with RdRP-specific RNA sequences, henceforth called RdRP/RNA promoters. The RdRP chosen for our project is taken from the Hepatitis C virus (HCV). The B2 RNA promoter was identified as such by Heinz, Kao (2000) , along with a number of other sequences derived from the Brome Mosaic Virus (BMV). All of them possess a few common characteristics: an initiation cytidylate at the 3??? end, where the replication starts; and a stable secondary structure ??? single stranded tail and a stem of various length. Replication stops at the 5??? end. Please refer to Section: RdRP-directed Replication for further details.
This is derived from the 3??? end of the Cytomegalovirus RNA3 minus strand. It contains a non-templated guanylate at the 3??? end. These are genomic plus-strand RNAs that can direct BMV minus-strand initiation. This has a 3??? single stranded tail which is required for efficient RNA synthesis.
false
false
_1820_
0
20675
9
Not in stock
true
Gel electrophoresis showing replication by HCV RdRP of a number of potential RNA promoters. The bands show the amount of replicated product and its approximate length, and quantify the tendency promoters to produce strands of wrong size. C2 (band 8) shows considerable efficiency in directing replication of the full template, although an amount of incorrect products were also present.
false
Iva Burova
annotation2420903
1
RNA Promoter
range2420903
1
7
33
annotation2420832
1
Ribozyme
range2420832
1
34
105
BBa_K1442115_sequence
1
cttaagggattgaacctcgttccgtggtttacgggccggcatggtcccagcctcctcgctggcgccggctgggcaacaccattgcactccggtggcgaatgggaccgtacg
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z