BBa_K1442117 1 BBa_K1442117 Sldel+8 3 2014-10-15T11:00:00Z 2015-05-08T01:10:27Z Brome Mosaic Virus Each unique RNA dependent RNA polymerase (RdRP) initiates de novo replication of a RNA strand by interacting with RdRP-specific RNA sequences, henceforth called RdRP/RNA promoters. The RdRP chosen for our project is taken from the Hepatitis C virus (HCV). The SLdel+8 RNA promoter was identified as such by Heinz, Kao (2000) , along with a number of other sequences. All of them possess a few common characteristics: an initiation cytidylate at the 3??? end, where the replication starts; and a stable secondary structure- single stranded tail and a stem of various length. Replication stops at the 5??? end. Please refer to Section: RdRP function for further details. This is a genomic plus-strand RNA that can direct BMV minus-strand initiation. This has 8 nucleotides at the 5???end deleted and shows that the 5??? end is not essential for NS5B action. false false _1820_ 0 20675 9 Not in stock true Gel electrophoresis showing replication by HCV RdRP of a number of potential RNA promoters. The bands show the amount of replicated product and its approximate length, and quantify the tendency to produce strands of wrong size. SLdel+8 (lane 10) does not perform very well in directing replication of a correct sized template and produces a large amount of faulty products. false Iva Burova annotation2421096 1 RNA Promoter range2421096 1 7 44 annotation2421097 1 Ribozyme range2421097 1 45 114 BBa_K1442117_sequence 1 agcttaagggcttgcatagcaagtctagatatgcgtccagagaccaggccggcatggtcccagcctcctcgctggcgccggctgggcaacaccattgcactccggtggcgaatgggaccgtacg igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z