LOCUS       BBa_K1442308             783 bp    DNA     linear       25-Nov-2024
DEFINITION  GFP codon optimised for human cells
ACCESSION   BBa_K1442308
VERSION     BBa_K1442308.1  
FEATURES             Location/Qualifiers
     misc_feature    1..8
                     /label=AscI resctriction site
     misc_feature    21..26
                     /label=BsrGI restriction site 
     CDS             27..746
                     /label=GFP codon optimised for humans
     misc_feature    741..743
                     /label=Stop codon
     misc_feature    744..746
                     /label=Stop codon
     misc_feature    747..754
                     /label=MauBI restriciton site 
     misc_feature    767..772
                     /label=MluI
     polyA_site      773..783
                     /label=poly A tail 
ORIGIN
        1 ggcgcgccat cgagttatgc tgtacaatgc ggaaagggga ggagctcttc acaggcgtgg
       61 tgcccatact ggtggagttg gacggcgacg tgaacggtca caagttctca gtctctgggg
      121 agggagaggg cgatgccacc tatggcaagc ttacactgaa gttcatttgt acgaccggca
      181 agctccctgt gccatggccc accctggtca caaccttcgg ctacggggtg cagtgctttg
      241 ccagatatcc cgaccacatg aagcagcatg acttctttaa gagtgcaatg cccgaagggt
      301 atgtgcagga acgaacgatt ttttttaagg acgacgggaa ttacaaaacc cgcgccgagg
      361 ttaagttcga gggcgatacc ctggttaatc ggatagagct caaaggaata gattttaaag
      421 aggacggtaa cattctgggc cacaagctcg aatataacta taattcacac aacgtataca
      481 tcatggctga caagcagaaa aacggcatca aagttaactt taagatacga cacaacattg
      541 aagacgggtc tgtgcagttg gctgaccact accagcagaa cacgccgatc ggggatggac
      601 cggtgctgct gcctgacaac cactacctct ctacccagtc tgccctgtct aaggacccta
      661 acgaaaagcg ggatcacatg gtgcttcttg agttcgtcac tgccgcagga attacacacg
      721 gtatggacga actctataag taataacgcg cgcgactgaa tgcatgacgc gtaaaaaaaa
      781 aaa
//