BBa_K090505
1
BBa_K090505
''Bacillus subtilis'' consensus RBS
2008-10-27T12:00:00Z
2015-05-08T01:08:37Z
This part was synthesized directly.
This is the consensus RBS for Bacillus subtilis.
false
false
_188_
0
3501
9
Not in stock
false
The part must be 8 nucleotides away from the first methionine of the coding sequence, and so we added 'TGT' downstream of the actual binding sequence: AAAGGAGG. This, combined with the truncated non-CDS BioBrick prefix scar, gives exactly 8 'spacer bases' between the 3' end of the RBS and the start of the coding sequence.
false
Daniel Goodman
annotation1992802
1
B. subtilis consensus RBS
range1992802
1
1
8
BBa_R0051
1
cI lam
promoter (lambda cI regulated)
2003-01-31T12:00:00Z
2015-05-08T01:14:14Z
<a href="http://www.nature.com/cgi-taf/DynaPage.taf?file=/nature/journal/v403/n6767/abs/403335a0_fs.html&dynoptions=doi1043774228">A synthetic oscillatory network of transcriptional regulators</a> , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000
Released HQ 2013
The cI regulated promoter is based on the pR promtoer from bacteriohage lambda. The promoter has two two DNA binding sites for lambda cI repressor <bb_part>BBa_C0051</bb_part>. cI binding results in repression of transcription. The specific sequence used here is based on the cI repressible promoter used in the Elowitz repressilator (and references therein).</P>
false
true
_1_
0
24
7
In stock
false
<P> <P>In order to address concerns about the promoter transcribing in the reverse direction, we have removed the -35 and -10 signals responsible for the promoter activity in the reverse direction. (<b><font color="red">More details needed here! DE, 2/24/03</font></b>)<P> Incompatible with host expressing cI repressor.
true
Vinay S Mahajan, Brian Chow, Peter Carr, Voichita Marinescu and Alexander D. Wissner-Gross
annotation7067
1
BBa_R0051
range7067
1
1
49
annotation2023
1
-35
range2023
1
15
20
annotation2024
1
OR1
range2024
1
25
41
annotation2025
1
OR2
range2025
1
1
17
annotation2022
1
-10
range2022
1
38
43
BBa_K823003
1
BBa_K823003
P<sub>veg</sub>
2012-07-15T11:00:00Z
2015-05-08T01:13:29Z
Bacillus subtilis
Released HQ 2013
Pveg is a strong, constitutive promoter of Bacillus subtilis
false
false
_1081_
0
12081
9
In stock
false
no considerations
false
Korinna Kraft
annotation2190180
1
promoter
range2190180
1
1
237
BBa_K1444002
1
BBa_K1444002
Composite promoter and consensus <i>B.subtilis</i> RBS - <i>C1-λ</i>
2014-10-07T11:00:00Z
2015-05-08T01:10:27Z
B. subtilis
A composite part consisting of the constitutive promoter Pveg (BBa_K823003), followed by of the repressible promoter C1-lambda (BBa_R0051), and ending with the consensus RBS from B. subtilis (BBa_K090505).
This composite part was intended to control the expression of a downstream reporter, regulated by the expression of the pLacI repressor protein. The constitutive promoter Pveg allows reliable expression when the repressible promoter is not repressed.
false
false
_1822_
0
20978
9
It's complicated
false
No interference with endogenous cell signalling.
false
Dan Ziemianowicz
component2422419
1
BBa_R0051
component2422417
1
BBa_K823003
component2422424
1
BBa_K090505
annotation2422424
1
BBa_K090505
range2422424
1
303
313
annotation2422419
1
BBa_R0051
range2422419
1
246
294
annotation2422417
1
BBa_K823003
range2422417
1
1
237
BBa_K823003_sequence
1
ggagttctgagaattggtatgccttataagtccaattaacagttgaaaacctgcataggagagctatgcgggttttttattttacataatgatacataatttaccgaaacttgcggaacataattgaggaatcatagaattttgtcaaaataattttattgacaacgtcttattaacgttgatataatttaaattttatttgacaaaaatgggctcgtgttgtacaataaatgtagt
BBa_R0051_sequence
1
taacaccgtgcgtgttgactattttacctctggcggtgataatggttgc
BBa_K1444002_sequence
1
ggagttctgagaattggtatgccttataagtccaattaacagttgaaaacctgcataggagagctatgcgggttttttattttacataatgatacataatttaccgaaacttgcggaacataattgaggaatcatagaattttgtcaaaataattttattgacaacgtcttattaacgttgatataatttaaattttatttgacaaaaatgggctcgtgttgtacaataaatgtagttactagagtaacaccgtgcgtgttgactattttacctctggcggtgataatggttgctactagagaaaggaggtgt
BBa_K090505_sequence
1
aaaggaggtgt
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z