BBa_R0052
1
cI 434
Promoter (434 cI regulated)
2003-01-31T12:00:00Z
2015-05-08T01:14:14Z
Bacteriophage 434 right operator
Released HQ 2013
The 434 cI regulatory region sequence is a 89 base-pair sequence with the standard BioBrick prefix and suffix sections on its ends. 434 cI repressor protein, <bb_part>BBa_C0052<bb_part>, binds to it.<br> This segment contains O-R1, O-R2, P-R, and P-RM -35.</p>
false
false
_1_
0
24
7
In stock
false
<P> <P><P>
true
Maia Mahoney
annotation2027
1
OR2
range2027
1
8
21
annotation2032
1
start
range2032
1
35
37
annotation2028
1
OR1
range2028
1
30
43
annotation7068
1
BBa_R0052
range7068
1
1
46
annotation2029
1
-35
range2029
1
1
6
annotation2026
1
OR2
range2026
1
1
43
annotation2030
1
-10
range2030
1
24
29
annotation2031
1
-35
range2031
1
2
7
BBa_K090505
1
BBa_K090505
''Bacillus subtilis'' consensus RBS
2008-10-27T12:00:00Z
2015-05-08T01:08:37Z
This part was synthesized directly.
This is the consensus RBS for Bacillus subtilis.
false
false
_188_
0
3501
9
Not in stock
false
The part must be 8 nucleotides away from the first methionine of the coding sequence, and so we added 'TGT' downstream of the actual binding sequence: AAAGGAGG. This, combined with the truncated non-CDS BioBrick prefix scar, gives exactly 8 'spacer bases' between the 3' end of the RBS and the start of the coding sequence.
false
Daniel Goodman
annotation1992802
1
B. subtilis consensus RBS
range1992802
1
1
8
BBa_K1444008
1
BBa_K1444008
Composite promoter and consensus B. subtilis RBS - C1-434
2014-10-07T11:00:00Z
2015-05-08T01:10:27Z
B. subtilis
A composite part consisting of the constitutive promoter Pveg (BBa_K823003), followed by the repressible promoter C1-434 (BBa_R0052), and ending with the consensus RBS from B. subtilis (BBa_K090505).
This composite part was intended to control the expression of a downstream reporter, regulated by the expression of the pLacI repressor protein. The constitutive promoter Pveg allows reliable expression when the repressible promoter is not repressed.
false
false
_1822_
0
20978
9
It's complicated
false
No interference with endogenous cell signalling.
false
Dan Ziemianowicz
component2405216
1
BBa_K823003
component2405226
1
BBa_K090505
component2405219
1
BBa_R0052
annotation2405216
1
BBa_K823003
range2405216
1
1
237
annotation2405226
1
BBa_K090505
range2405226
1
300
310
annotation2405219
1
BBa_R0052
range2405219
1
246
291
BBa_K823003
1
BBa_K823003
P<sub>veg</sub>
2012-07-15T11:00:00Z
2015-05-08T01:13:29Z
Bacillus subtilis
Released HQ 2013
Pveg is a strong, constitutive promoter of Bacillus subtilis
false
false
_1081_
0
12081
9
In stock
false
no considerations
false
Korinna Kraft
annotation2190180
1
promoter
range2190180
1
1
237
BBa_K823003_sequence
1
ggagttctgagaattggtatgccttataagtccaattaacagttgaaaacctgcataggagagctatgcgggttttttattttacataatgatacataatttaccgaaacttgcggaacataattgaggaatcatagaattttgtcaaaataattttattgacaacgtcttattaacgttgatataatttaaattttatttgacaaaaatgggctcgtgttgtacaataaatgtagt
BBa_R0052_sequence
1
ttgacaaacaagatacattgtatgaaaatacaagaaagtttgttga
BBa_K1444008_sequence
1
ggagttctgagaattggtatgccttataagtccaattaacagttgaaaacctgcataggagagctatgcgggttttttattttacataatgatacataatttaccgaaacttgcggaacataattgaggaatcatagaattttgtcaaaataattttattgacaacgtcttattaacgttgatataatttaaattttatttgacaaaaatgggctcgtgttgtacaataaatgtagttactagagttgacaaacaagatacattgtatgaaaatacaagaaagtttgttgatactagagaaaggaggtgt
BBa_K090505_sequence
1
aaaggaggtgt
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z