LOCUS       BBa_K1444015             464 bp    DNA     linear       26-Dec-2024
DEFINITION  Composite promoter and consensus B. subtilis RBS - pLacI
ACCESSION   BBa_K1444015
VERSION     BBa_K1444015.1  
FEATURES             Location/Qualifiers
     promoter        1..237
                     /label=promoter
     promoter        1..237
                     /label=BBa_K823003
     CDS             246..333
                     /label=end of LacI coding region (inactive)
     misc_feature    246..445
                     /label=BBa_R0010
     misc_feature    334..371
                     /label=CAP binding site
     promoter        382..387
                     /label=-35
     promoter        406..411
                     /label=-10
     misc_feature    411..445
                     /label=LacI binding site
     misc_feature    418..418
                     /label=start
     promoter        246..445
                     /label=BBa_R0010
     RBS             454..461
                     /label=B. subtilis consensus RBS
     RBS             454..464
                     /label=BBa_K090505
ORIGIN
        1 ggagttctga gaattggtat gccttataag tccaattaac agttgaaaac ctgcatagga
       61 gagctatgcg ggttttttat tttacataat gatacataat ttaccgaaac ttgcggaaca
      121 taattgagga atcatagaat tttgtcaaaa taattttatt gacaacgtct tattaacgtt
      181 gatataattt aaattttatt tgacaaaaat gggctcgtgt tgtacaataa atgtagttac
      241 tagagcaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      301 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
      361 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
      421 tgtgagcgga taacaatttc acacatacta gagaaaggag gtgt
//