LOCUS       BBa_K1444016             880 bp    DNA     linear       22-Dec-2024
DEFINITION  Composite promoter and consensus B. subtilis RBS - pLacI
ACCESSION   BBa_K1444016
VERSION     BBa_K1444016.1  
FEATURES             Location/Qualifiers
     promoter        1..237
                     /label=promoter
     promoter        1..237
                     /label=BBa_K823003
     CDS             246..333
                     /label=end of LacI coding region (inactive)
     misc_feature    246..445
                     /label=BBa_R0010
     misc_feature    334..371
                     /label=CAP binding site
     promoter        382..387
                     /label=-35
     promoter        406..411
                     /label=-10
     misc_feature    411..445
                     /label=LacI binding site
     misc_feature    418..418
                     /label=start
     promoter        246..445
                     /label=BBa_R0010
     CDS             454..541
                     /label=end of LacI coding region (inactive)
     misc_feature    454..653
                     /label=BBa_R0010
     misc_feature    542..579
                     /label=CAP binding site
     promoter        590..595
                     /label=-35
     promoter        614..619
                     /label=-10
     misc_feature    619..653
                     /label=LacI binding site
     misc_feature    626..626
                     /label=start
     promoter        454..653
                     /label=BBa_R0010
     CDS             662..749
                     /label=end of LacI coding region (inactive)
     misc_feature    662..861
                     /label=BBa_R0010
     misc_feature    750..787
                     /label=CAP binding site
     promoter        798..803
                     /label=-35
     promoter        822..827
                     /label=-10
     misc_feature    827..861
                     /label=LacI binding site
     misc_feature    834..834
                     /label=start
     promoter        662..861
                     /label=BBa_R0010
     RBS             870..877
                     /label=B. subtilis consensus RBS
     RBS             870..880
                     /label=BBa_K090505
ORIGIN
        1 ggagttctga gaattggtat gccttataag tccaattaac agttgaaaac ctgcatagga
       61 gagctatgcg ggttttttat tttacataat gatacataat ttaccgaaac ttgcggaaca
      121 taattgagga atcatagaat tttgtcaaaa taattttatt gacaacgtct tattaacgtt
      181 gatataattt aaattttatt tgacaaaaat gggctcgtgt tgtacaataa atgtagttac
      241 tagagcaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      301 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
      361 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
      421 tgtgagcgga taacaatttc acacatacta gagcaatacg caaaccgcct ctccccgcgc
      481 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
      541 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
      601 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acatactaga
      661 gcaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
      721 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact
      781 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg
      841 agcggataac aatttcacac atactagaga aaggaggtgt
//