BBa_K1444016
1
BBa_K1444016
Composite promoter and consensus B. subtilis RBS - pLacI
2014-10-07T11:00:00Z
2015-05-08T01:10:27Z
B. subtilis
A composite part consisting of the constitutive promoter Pveg (BBa_K823003), followed by three copies of the repressible promoter pLacI (BBa_R0010), and ending with the consensus RBS from B. subtilis (BBa_K090505).
This composite part was intended to control the expression of a downstream reporter, regulated by the expression of the pLacI repressor protein. The constitutive promoter Pveg allows reliable expression when the repressible promoter is not repressed. The triplicate repressible promoter allows for stronger repression.
false
false
_1822_
0
20978
9
It's complicated
false
No interference with endogenous cell signalling.
false
Dan Ziemianowicz
component2405411
1
BBa_R0010
component2405396
1
BBa_K823003
component2405397
1
BBa_R0010
component2405419
1
BBa_K090505
component2405404
1
BBa_R0010
annotation2405396
1
BBa_K823003
range2405396
1
1
237
annotation2405411
1
BBa_R0010
range2405411
1
662
861
annotation2405404
1
BBa_R0010
range2405404
1
454
653
annotation2405419
1
BBa_K090505
range2405419
1
870
880
annotation2405397
1
BBa_R0010
range2405397
1
246
445
BBa_K090505
1
BBa_K090505
''Bacillus subtilis'' consensus RBS
2008-10-27T12:00:00Z
2015-05-08T01:08:37Z
This part was synthesized directly.
This is the consensus RBS for Bacillus subtilis.
false
false
_188_
0
3501
9
Not in stock
false
The part must be 8 nucleotides away from the first methionine of the coding sequence, and so we added 'TGT' downstream of the actual binding sequence: AAAGGAGG. This, combined with the truncated non-CDS BioBrick prefix scar, gives exactly 8 'spacer bases' between the 3' end of the RBS and the start of the coding sequence.
false
Daniel Goodman
annotation1992802
1
B. subtilis consensus RBS
range1992802
1
1
8
BBa_R0010
1
LacI
promoter (lacI regulated)
2003-01-31T12:00:00Z
2015-05-08T01:14:14Z
The Plac insert was PCR'd from the MG1655 strain of E.coli K12.
Released HQ 2013
Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) <p> The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG.
false
true
_1_
0
24
7
In stock
false
<P> <P><P> LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs.
true
annotation1961223
1
CAP binding site
range1961223
1
89
126
annotation1961227
1
start
range1961227
1
173
173
annotation1961222
1
BBa_R0010
range1961222
1
1
200
annotation1961225
1
-10
range1961225
1
161
166
annotation1961226
1
LacI binding site
range1961226
1
166
200
annotation1961224
1
-35
range1961224
1
137
142
annotation1961221
1
end of LacI coding region (inactive)
range1961221
1
1
88
BBa_K823003
1
BBa_K823003
P<sub>veg</sub>
2012-07-15T11:00:00Z
2015-05-08T01:13:29Z
Bacillus subtilis
Released HQ 2013
Pveg is a strong, constitutive promoter of Bacillus subtilis
false
false
_1081_
0
12081
9
In stock
false
no considerations
false
Korinna Kraft
annotation2190180
1
promoter
range2190180
1
1
237
BBa_R0010_sequence
1
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca
BBa_K1444016_sequence
1
ggagttctgagaattggtatgccttataagtccaattaacagttgaaaacctgcataggagagctatgcgggttttttattttacataatgatacataatttaccgaaacttgcggaacataattgaggaatcatagaattttgtcaaaataattttattgacaacgtcttattaacgttgatataatttaaattttatttgacaaaaatgggctcgtgttgtacaataaatgtagttactagagcaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagcaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagcaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaaggaggtgt
BBa_K823003_sequence
1
ggagttctgagaattggtatgccttataagtccaattaacagttgaaaacctgcataggagagctatgcgggttttttattttacataatgatacataatttaccgaaacttgcggaacataattgaggaatcatagaattttgtcaaaataattttattgacaacgtcttattaacgttgatataatttaaattttatttgacaaaaatgggctcgtgttgtacaataaatgtagt
BBa_K090505_sequence
1
aaaggaggtgt
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z