LOCUS       BBa_K1444027            2647 bp    DNA     linear       22-Nov-2024
DEFINITION  pUC57 cloning vector from BioBasic
ACCESSION   BBa_K1444027
VERSION     BBa_K1444027.1  
FEATURES             Location/Qualifiers
     CDS             146..430
                     /label=LacZ alpha
     misc_feature    396..413
                     /label=Cloning site
     misc_feature    810..1492
                     /label=ColE1 replication origin
     misc_feature    1590..2249
                     /label=ampR
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtaggcc tgcatgcaag cttggcgtaa
      421 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
      481 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
      541 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
      601 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
      661 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
      721 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
      781 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
      841 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
      901 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
      961 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     1021 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     1081 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     1141 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     1201 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     1261 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     1321 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     1381 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     1441 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     1501 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     1561 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     1621 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     1681 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     1741 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     1801 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     1861 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     1921 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     1981 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     2041 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     2101 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     2161 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     2221 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     2281 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     2341 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     2401 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     2461 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     2521 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     2581 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
     2641 tttcgtc
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