LOCUS BBa_K1445001 5617 bp DNA linear 14-Nov-2024 DEFINITION Endogenous Type II CRISPR-Cas9 phagemid ACCESSION BBa_K1445001 VERSION BBa_K1445001.1 FEATURES Location/Qualifiers misc_feature 1..529 /label=BBa_K1445000 CDS 538..5617 /label=BBa_K1218011 ORIGIN 1 attgatttac cccggttgat aatcagaaaa gccccaaaaa caggaagatt gtataagcaa 61 atatttaaat tgtaaacgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca 121 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaatagc 181 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg 241 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat 301 cacccaaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag 361 ggagcccccg atttagagct tgacggggaa agcgaacgtg gcgagaaagg aagggaagaa 421 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac 481 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtaa aaggatctat actagagtta 541 cgaaatcatc ctgtggagct tagtaggttt agcaagatgg cagcgcctaa atgtagaatg 601 ataaaaggat taagagatta atttccctaa aaatgataaa acaagcgttt tgaaagcgct 661 tgtttttttg gtttgcagtc agagtagaat agaagtatca aaaaaagcac cgactcggtg 721 ccactttttc aagttgataa cggactagcc ttattttaac ttgctatgct gttttgaatg 781 gttccaacaa gattatttta taacttttat aacaaataat caaggagaaa ttcaaagaaa 841 tttatcagcc ataaaacaat acttaatact atagaatgat aacaaaataa actacttttt 901 aaaagaattt tgtgttataa tctatttatt attaagtatt gggtaatatt ttttgaagag 961 atattttgaa aaagaaaaat taaagcatat taaactaatt tcggaggtca ttaaaactat 1021 tattgaaatc atcaaactca ttatggattt aatttaaact ttttatttta ggaggcaaaa 1081 atggataaga aatactcaat aggcttagat atcggcacaa atagcgtcgg atgggcggtg 1141 atcactgatg aatataaggt tccgtctaaa aagttcaagg ttctgggaaa tacagaccgc 1201 cacagtatca aaaaaaatct tataggggct cttttatttg acagtggaga gacagcggaa 1261 gcgactcgtc tcaaacggac agctcgtaga aggtatacac gtcggaagaa tcgtatttgt 1321 tatctacagg agattttttc aaatgagatg gcgaaagtag atgatagttt ctttcatcga 1381 cttgaagagt cttttttggt ggaagaagac aagaagcatg aacgtcatcc tatttttgga 1441 aatatagtag atgaagttgc ttatcatgag aaatatccaa ctatctatca tctgcgaaaa 1501 aaattggtag attctactga taaagcggat ttgcgcttaa tctatttggc cttagcgcat 1561 atgattaagt ttcgtggtca ttttttgatt gagggagatt taaatcctga taatagtgat 1621 gtggacaaac tatttatcca gttggtacaa acctacaatc aattatttga agaaaaccct 1681 attaacgcaa gtggagtaga tgctaaagcg attctttctg cacgattgag taaatcaaga 1741 cgattagaaa atctcattgc tcagctcccc ggtgagaaga aaaatggctt atttgggaat 1801 ctcattgctt tgtcattggg tttgacccct aattttaaat caaattttga tttggcagaa 1861 gatgctaaat tacagctttc aaaagatact tacgatgatg atttagataa tttattggcg 1921 caaattggag atcaatatgc tgatttgttt ttggcagcta agaatttatc agatgctatt 1981 ttactttcag atatcctaag agtaaatact gaaataacta aggctcccct atcagcttca 2041 atgattaaac gctacgatga acatcatcaa gacttgactc ttttaaaagc tttagttcga 2101 caacaacttc cagaaaagta taaagaaatc ttttttgatc aatcaaaaaa cggatatgca 2161 ggttatattg atgggggagc tagccaagaa gaattttata aatttatcaa accaatttta 2221 gaaaaaatgg atggtactga ggaattattg gtgaaactaa atcgtgaaga tttgctgcgc 2281 aagcaacgga cctttgacaa cggctctatt ccccatcaaa ttcacttggg tgagctgcat 2341 gctattttga gaagacaaga agacttttat ccatttttaa aagacaatcg tgagaagatt 2401 gaaaaaatct tgacttttcg tattccttat tatgttggtc cattggcgcg tggcaatagt 2461 cgttttgcat ggatgactcg gaagtctgaa gaaacaatta ccccatggaa ttttgaagaa 2521 gttgtcgata aaggtgcttc agctcaatca tttattgaac gcatgacaaa ctttgataaa 2581 aatcttccaa atgaaaaagt actaccaaaa catagtttgc tttatgagta ttttacggtt 2641 tataacgaat tgacaaaggt caaatatgtt actgaaggaa tgcgaaaacc agcatttctt 2701 tcaggtgaac agaagaaagc cattgttgat ttactcttca aaacaaatcg aaaagtaacc 2761 gttaagcaat taaaagaaga ttatttcaaa aaaatagaat gttttgatag tgttgaaatt 2821 tcaggagttg aagatagatt taatgcttca ttaggtacct accatgattt gctaaaaatt 2881 attaaagata aagatttttt ggataatgaa gaaaatgaag atatcttaga ggatattgtt 2941 ttaacattga ccttatttga agatagggag atgattgagg aaagacttaa aacatatgct 3001 cacctctttg atgataaggt gatgaaacag cttaaacgtc gccgttatac tggttgggga 3061 cgtttgtctc gaaaattgat taatggtatt agggataagc aatctggcaa aacaatatta 3121 gattttttga aatcagatgg ttttgccaat cgcaatttta tgcagctgat ccatgatgat 3181 agtttgacat ttaaagaaga cattcaaaaa gcacaagtgt ctggacaagg cgatagttta 3241 catgaacata ttgcaaattt agctggtagc cctgctatta aaaaaggtat tttacagact 3301 gtaaaagttg ttgatgaatt ggtcaaagta atggggcggc ataagccaga aaatatcgtt 3361 attgaaatgg cacgtgaaaa tcagacaact caaaagggcc agaaaaattc gcgagagcgt 3421 atgaaacgaa tcgaagaagg tatcaaagaa ttaggaagtc agattcttaa agagcatcct 3481 gttgaaaata ctcaattgca aaatgaaaag ctctatctct attatctcca aaatggaaga 3541 gacatgtatg tggaccaaga attagatatt aatcgtttaa gtgattatga tgtcgatcac 3601 attgttccac aaagtttcct taaagacgat tcaatagaca ataaggtctt aacgcgttct 3661 gataaaaatc gtggtaaatc ggataacgtt ccaagtgaag aagtagtcaa aaagatgaaa 3721 aactattgga gacaacttct aaacgccaag ttaatcactc aacgtaagtt tgataattta 3781 acgaaagctg aacgtggagg tttgagtgaa cttgataaag ctggttttat caaacgccaa 3841 ttggttgaaa ctcgccaaat cactaagcat gtggcacaaa ttttggatag tcgcatgaat 3901 actaaatacg atgaaaatga taaacttatt cgagaggtta aagtgattac cttaaaatct 3961 aaattagttt ctgacttccg aaaagatttc caattctata aagtacgtga gattaacaat 4021 taccatcatg cccatgatgc gtatctaaat gccgtcgttg gaactgcttt gattaagaaa 4081 tatccaaaac ttgaatcgga gtttgtctat ggtgattata aagtttatga tgttcgtaaa 4141 atgattgcta agtctgagca agaaataggc aaagcaaccg caaaatattt cttttactct 4201 aatatcatga acttcttcaa aacagaaatt acacttgcaa atggagagat tcgcaaacgc 4261 cctctaatcg aaactaatgg ggaaactgga gaaattgtct gggataaagg gcgagatttt 4321 gccacagtgc gcaaagtatt gtccatgccc caagtcaata ttgtcaagaa aacagaagta 4381 cagacaggcg gattctccaa ggagtcaatt ttaccaaaaa gaaattcgga caagcttatt 4441 gctcgtaaaa aagactggga tccaaaaaaa tatggtggtt ttgatagtcc aacggtagct 4501 tattcagtcc tagtggttgc taaggtggaa aaagggaaat cgaagaagtt aaaatccgtt 4561 aaagagttac tagggatcac aattatggaa agaagttcct ttgaaaaaaa tccgattgac 4621 tttttagaag ctaaaggata taaggaagtt aaaaaagact taatcattaa actacctaaa 4681 tatagtcttt ttgagttaga aaacggtcgt aaacggatgc tggctagtgc cggagaatta 4741 caaaaaggaa atgagctggc tctgccaagc aaatatgtga attttttata tttagctagt 4801 cattatgaaa agttgaaggg tagtccagaa gataacgaac aaaaacaatt gtttgtggag 4861 cagcataagc attatttaga tgagattatt gagcaaatca gtgaattttc taagcgtgtt 4921 attttagcag atgccaattt agataaagtt cttagtgcat ataacaaaca tagagacaaa 4981 ccaatacgtg aacaagcaga aaatattatt catttattta cgttgacgaa tcttggagct 5041 cccgctgctt ttaaatattt tgatacaaca attgatcgta aacgatatac gtctacaaaa 5101 gaagttttag atgccactct tatccatcaa tccatcactg gtctttatga aacacgcatt 5161 gatttgagtc agctaggagg tgactgaagt atattttaga tgaagattat ttcttaataa 5221 ctaaaaatat ggtataatac tcttaataaa tgcagtaata caggggcttt tcaagactga 5281 agtctagctg agacaaatag tgcgattacg aaatttttta gacaaaaata gtctacgagg 5341 ttttagagct atgctgtttt gaatggtccc aaaactgaga ccagtctcgg aagctcaaag 5401 gtctcgtttt agagctatgc tgttttgaat ggtcccaaaa cttcagcaca ctgagacttg 5461 ttgagttcca tgttttagag ctatgctgtt ttgaatggac tccattcaac attgccgatg 5521 ataacttgag aaagagggtt aataccagca gtcggatacc ttcctattct ttctgttaaa 5581 gcgttttcat gttataatag gcaaaagaag agtagtg //