BBa_K1446001 1 BBa_K1446001 dcmA-gene with N-terminal pdu-microcompartment tag, codon optimised for E.coli (K12) 2014-09-28T11:00:00Z 2015-05-08T01:10:27Z The sequence comes from the original gene in the genomic DNA of DM4. We have taken the seuqence from uniprot. dcmA is a gene coding for the enzyme dichloromethane dehalogenase from Methylobacterium extorquens (DM4). This enzyme is a member of the glutathione-dependent-S-transferase family and catalyses the following reaction: DCM (dichloromethane) + 2H2O -> formaldehyde + 2 HCl We have attached the N-terminal targeting sequence for the pdu-microcomartment to this part in order to allow for the insertion of this enzyme into the microcompartment. false false _1824_ 0 19947 9 It's complicated true The wild-type dcmA-gene has two EcoRI-restriction sites. We have therefore changed the restriction site sequences from GAATTC to GAATTT in order to make this part BioBrick compatible. false Philipp Lorenz annotation2389087 1 dcmA from M. extorquens, codon optimised for E.coli K12 range2389087 1 52 830 annotation2389086 1 N-terminal pdu-microcompartment tag range2389086 1 4 51 BBa_K1446001_sequence 1 catatgaacaccagtgaactggaaaccttaatccgtaccatcctgtctgaacagctgtatgaaattgatgtcccattcgaagaagaagtagttgacatcagcaccgatattacggaacgccaggaatttcgcgacaaatataacccgaccggtcaagtgccgatcctggtagatggcgaatttaccgtttgggaaagcgttgcgatcgcgcgctacgttaacgaaaaatttgatggtgccggcaactggttcggccgcggtactcaggaacgcgcgcagattaatcagtttcttcagtggtatgcttacaccctgcgcctgggtggtggtgccttccattggaacatcttcgggtgcctgatttacggtgaaaaaccgtatagcccgaaattcacggcagaacagaacaagggtcgtacgctgctgtacgaagcgatgggcaccctggaaaactattggctccgtgatcgtgaatatgtttgcggcgatgaagtttcgtacgcggacctggcggctttccacgaatttgtgtctcacgaagctggtaaaatcatcccggaccgagtgtggcagggtttcccgaaaatcgcggcctggttcaaaaaactgtctgaacgtccacatgcaaaaaccgtctctgagtggcagtataccaacgttggtaaaatcatccgtggtgaattaaccgcgagcatgtttaaacgtaaaacggcagtcctgaaaggcacagaagtattcagcggccataaccacggtattccgtatcttaacgaaaaagcggaagactattttcaaacgtgtagaaaaggaaggggctgcggtggccttag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z