BBa_K1446002 1 BBa_K1446002 superfolder GFP with N-terminal pdu-microcompartment tag 2014-09-28T11:00:00Z 2015-05-08T01:10:27Z We received a sample of sfGFP in a holding vector, provided by Dr. Ciaran Kelly. We have used superfolded GFP with the N-terminal pdu-microcompartment tag in order to get fluorescence images that would show localised spots of higher GFP-density, indicating that the microcompartment tag successfully inserts the attached protein into the microcompartments false false _1824_ 0 19947 9 In stock false We have designed Primers to amplify sfGFP from a plasmid, provided by Dr. Ciaran Kelly, in order to attach the microcompartment tag upstream of the sfGFP-coding region. false Philipp Lorenz annotation2389096 1 superfolder GFP coding sequence range2389096 1 81 831 annotation2389094 1 ribosome binding site range2389094 1 14 19 annotation2389095 1 pdu-microcompartment tag range2389095 1 31 80 BBa_K1446002_sequence 1 tttcatattttctaggaggcagaagttatgaacacctcggaactggaaaccctgatccgcaccatccttagcgagcaactgcgtaaaggcgaagagctgttcactggtgtcgtccctattctggtggaactggatggtgatgtcaacggtcataagttttccgtgcgtggcgagggtgaaggtgacgcaactaatggtaaactgacgctgaagttcatctgtactactggtaaactgccggtaccttggccgactctggtaacgacgctgacttatggtgttcagtgctttgctcgttatccggaccatatgaagcagcatgacttcttcaagtccgccatgccggaaggctatgtgcaggaacgcacgatttcctttaaggatgacggcacgtacaaaacgcgtgcggaagtgaaatttgaaggcgataccctggtaaaccgcattgagctgaaaggcattgactttaaagaagacggcaatatcctgggccataagctggaatacaattttaacagccacaatgtttacatcaccgccgataaacaaaaaaatggcattaaagcgaattttaaaattcgccacaacgtggaggatggcagcgtgcagctggctgatcactaccagcaaaacactccaatcggtgatggtcctgttctgctgccagacaatcactatctgagcacgcaaagcgttctgtctaaagatccgaacgagaaacgcgatcatatggttctgctggagttcgtaaccgcagcgggcatcacgcatggtatggatgaactgtacaaaggctccggctccggctcccaccatcaccatcaccattga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z