##gff-version 3
##sequence-region BBa_K145000 1 1033
BBa_K145000	.	ribosome_entry_site	117	128	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K145000	.	conserved_region	121	124	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K145000	.	terminator	993	1033	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K145000	.	polyA_site	1020	1033	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K145000	.	stop_codon	1026	1026	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K145000	.	stem_loop	1000	1019	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K145000	.	engineered_region	993	1033	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K145000	.	terminator	905	984	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K145000	.	stem_loop	916	959	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K145000	.	engineered_region	905	984	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K145000	.	engineered_region	135	896	.	+	0	ID=BBa_E0022;Name=BBa_E0022
BBa_K145000	.	engineered_region	135	896	.	+	0	ID=annotation7040;Name=BBa_E0022;Parent=BBa_E0022
BBa_K145000	.	tag	853	890	.	+	0	ID=annotation2153;Name=SsrA;Parent=BBa_E0022
BBa_K145000	.	stop_codon	891	896	.	+	0	ID=annotation2154;Name=2;Parent=BBa_E0022
BBa_K145000	.	CDS	135	896	.	+	0	ID=annotation2150;Name=CFP (LVA);Parent=BBa_E0022
BBa_K145000	.	point_mutation	203	203	.	+	0	ID=annotation2155;Name=A;Parent=BBa_E0022
BBa_K145000	.	promoter	1	108	.	+	0	ID=BBa_R0084;Name=BBa_R0084
BBa_K145000	.	non_covalent_binding_site	51	68	.	+	0	ID=annotation301313;Name=F3 OmpR;Parent=BBa_R0084
BBa_K145000	.	non_covalent_binding_site	31	48	.	+	0	ID=annotation301309;Name=F2 OmpR;Parent=BBa_R0084
BBa_K145000	.	promoter	73	78	.	+	0	ID=annotation301317;Name=-35;Parent=BBa_R0084
BBa_K145000	.	non_covalent_binding_site	11	28	.	+	0	ID=annotation301308;Name=F1 OmpR;Parent=BBa_R0084
BBa_K145000	.	promoter	88	93	.	+	0	ID=annotation301318;Name=-10;Parent=BBa_R0084
>BBa_K145000
cttaaattttacttttggttacatattttttctttttgaaaccaaatctttatctttgtagcactttcacggtagcgaa
acgttagtttgaatggaaagatgcctgcatactagagaaagaggagaaatactagatggtgagcaagggcgaggagctg
ttcaccggggtggtgcccatcctggtcgagctggacggcgacgtgaacggccacaagttcagcgtgtccggcgagggcg
agggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccct
cgtgaccaccctgacctggggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtcc
gccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtga
agttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggca
caagctggagtacaactacatcagccacaacgtctatatcaccgccgacaagcagaagaacggcatcaaggccaacttc
aagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggcc
ccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacat
ggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagaggcctgctgcaaacgac
gaaaactacgctttagtagcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcc
tttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcg
tttata