LOCUS       BBa_K1450003            2411 bp    DNA     linear       14-Nov-2024
DEFINITION  pBAD_T7 DNA polymerase
ACCESSION   BBa_K1450003
VERSION     BBa_K1450003.1  
FEATURES             Location/Qualifiers
     promoter        1..130
                     /label=BBa_I13453
     misc_feature    143..146
                     /label=conserved
     RBS             139..150
                     /label=BBa_B0034
     CDS             157..2274
                     /label=BBa_K1450000
     stem_loop       2294..2337
                     /label=stem_loop
     misc_feature    2283..2362
                     /label=BBa_B0010
     terminator      2283..2362
                     /label=BBa_B0010
     stem_loop       2378..2397
                     /label=T7 TE
     misc_feature    2404..2404
                     /label=stop
     polyA_site      2398..2411
                     /label=polya
     misc_feature    2371..2411
                     /label=BBa_B0012
     terminator      2371..2411
                     /label=BBa_B0012
     terminator      2283..2411
                     /label=BBa_B0015
ORIGIN
        1 acattgatta tttgcacggc gtcacacttt gctatgccat agcattttta tccataagat
       61 tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca taccgttttt
      121 ttgggctagc tactagagaa agaggagaaa tactagatga tcgtttctga catcgaagct
      181 aacgccctct tagagagcgt cactaagttc cactgcgggg ttatctacga ctactccacc
      241 gctgagtacg taagctaccg tccgagtgac ttcggtgcgt atctggatgc gctggaagcc
      301 gaggttgcac gaggcggtct tattgtgttc cacaacggtc acaagtatga cgttcctgca
      361 ttgaccaaac tggcaaagtt gcaattgaac cgagagttcc accttcctcg tgagaactgt
      421 attgacaccc ttgtgttgtc acgtttgatt cattccaacc tcaaggacac cgatatgggt
      481 cttctgcgtt ccggcaagtt gcccggaaaa cgctttgggt ctcacgcttt ggaggcgtgg
      541 ggttatcgct taggcgagat gaagggtgaa tacaaagacg actttaagcg tatgcttgaa
      601 gagcagggtg aagaatacgt tgacggaatg gagtggtgga acttcaacga agagatgatg
      661 gactataacg ttcaggacgt tgtggtaact aaagctctcc ttgagaagct actctctgac
      721 aaacattact tccctcctga gattgacttt acggacgtag gatacactac gttctggtca
      781 gaatcccttg aggccgttga cattgaacat cgtgctgcat ggctgctcgc taaacaagag
      841 cgcaacgggt tcccgtttga cacaaaagca atcgaagagt tgtacgtaga gttagctgct
      901 cgccgctctg agttgctccg taaattgacc gaaacgttcg gctcgtggta tcagcctaaa
      961 ggtggcactg agatgttctg ccatccgcga acaggtaagc cactacctaa ataccctcgc
     1021 attaagacac ctaaagttgg tggtatcttt aagaagccta agaacaaggc acagcgagaa
     1081 ggccgtgagc cttgcgaact tgatacccgc gagtacgttg ctggtgctcc ttacacccca
     1141 gttgaacatg ttgtgtttaa cccttcgtct cgtgaccaca ttcagaagaa actccaagag
     1201 gctgggtggg tcccgaccaa gtacaccgat aagggtgctc ctgtggtgga cgatgaggta
     1261 ctcgaaggag tacgtgtaga tgaccctgag aagcaagccg ctatcgacct cattaaagag
     1321 tacttgatga ttcagaagcg aatcggacag tctgctgagg gagacaaagc atggcttcgt
     1381 tatgttgctg aggatggtaa gattcatggt tctgttaacc ctaatggagc agttacgggt
     1441 cgtgcgaccc atgcgttccc aaaccttgcg caaattccgg gtgtacgttc tccttatgga
     1501 gagcagtgtc gcgctgcttt tggcgctgag caccatttgg atgggataac tggtaagcct
     1561 tgggttcagg ctggcatcga cgcatccggt cttgagctac gctgcttggc tcacttcatg
     1621 gctcgctttg ataacggcga gtacgctcac gagattctta acggcgacat ccacactaag
     1681 aaccagatag ctgctgaact acctacccga gataacgcta agacgttcat ctatgggttc
     1741 ctctatggtg ctggtgatga gaagattgga cagattgttg gtgctggtaa agagcgcggt
     1801 aaggaactca agaagaaatt ccttgagaac acccccgcga ttgcagcact ccgcgagtct
     1861 atccaacaga cacttgtcga gtcctctcaa tgggtagctg gtgagcaaca agtcaagtgg
     1921 aaacgccgct ggattaaagg tctggatggt cgtaaggtac acgttcgtag tcctcacgct
     1981 gccttgaata ccctactgca atctgctggt gctctcatct gcaaactgtg gattatcaag
     2041 accgaagaga tgctcgtaga gaaaggcttg aagcatggct gggatgggga ctttgcgtac
     2101 atggcatggg tacatgatga aatccaagta ggctgccgta ccgaagagat tgctcaggtg
     2161 gtcattgaga ccgcacaaga agcgatgcgc tgggttggag accactggaa cttccggtgt
     2221 cttctggata ccgaaggtaa gatgggtcct aattgggcga tttgccactg ataatactag
     2281 agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg
     2341 ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt
     2401 ctgcgtttat a
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