LOCUS       BBa_K1450004            2948 bp    DNA     linear       29-Nov-2024
DEFINITION  pBAD_T7 RNA polymerase
ACCESSION   BBa_K1450004
VERSION     BBa_K1450004.1  
FEATURES             Location/Qualifiers
     promoter        1..130
                     /label=BBa_I13453
     misc_feature    143..146
                     /label=conserved
     RBS             139..150
                     /label=BBa_B0034
     CDS             157..2811
                     /label=BBa_K1450001
     stem_loop       2831..2874
                     /label=stem_loop
     misc_feature    2820..2899
                     /label=BBa_B0010
     terminator      2820..2899
                     /label=BBa_B0010
     stem_loop       2915..2934
                     /label=T7 TE
     misc_feature    2941..2941
                     /label=stop
     polyA_site      2935..2948
                     /label=polya
     misc_feature    2908..2948
                     /label=BBa_B0012
     terminator      2908..2948
                     /label=BBa_B0012
     terminator      2820..2948
                     /label=BBa_B0015
ORIGIN
        1 acattgatta tttgcacggc gtcacacttt gctatgccat agcattttta tccataagat
       61 tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca taccgttttt
      121 ttgggctagc tactagagaa agaggagaaa tactagatga acacgattaa catcgctaag
      181 aacgacttct ctgacatcga actggctgct atcccgttca acactctggc tgaccattac
      241 ggtgagcgtt tagctcgcga acagttggcc cttgagcatg agtcttacga gatgggtgaa
      301 gcacgcttcc gcaagatgtt tgagcgtcaa cttaaagctg gtgaggttgc ggataacgct
      361 gccgccaagc ctctcatcac taccctactc cctaagatga ttgcacgcat caacgactgg
      421 tttgaggaag tgaaagctaa gcgcggcaag cgcccgacag ccttccagtt cctgcaagaa
      481 atcaagccgg aagccgtagc gtacatcacc attaagacca ctctggcttg cctaaccagt
      541 gctgacaata caaccgttca ggctgtagca agcgcaatcg gtcgggccat tgaggacgag
      601 gctcgcttcg gtcgtatccg tgaccttgaa gctaagcact tcaagaaaaa cgttgaggaa
      661 caactcaaca agcgcgtagg gcacgtctac aagaaagcat ttatgcaagt tgtcgaggct
      721 gacatgctct ctaagggtct actcggtggc gaggcgtggt cttcgtggca taaggaagac
      781 tctattcatg taggagtacg ctgcatcgag atgctcattg agtcaaccgg aatggttagc
      841 ttacaccgcc aaaatgctgg cgtagtaggt caagactctg agactatcga actcgcacct
      901 gaatacgctg aggctatcgc aacccgtgca ggtgcgctgg ctggcatctc tccgatgttc
      961 caaccttgcg tagttcctcc taagccgtgg actggcatta ctggtggtgg ctattgggct
     1021 aacggtcgtc gtcctctggc gctggtgcgt actcacagta agaaagcact gatgcgctac
     1081 gaagacgttt acatgcctga ggtgtacaaa gcgattaaca ttgcgcaaaa caccgcatgg
     1141 aaaatcaaca agaaagtcct agcggtcgcc aacgtaatca ccaagtggaa gcattgtccg
     1201 gtcgaggaca tccctgcgat tgagcgtgaa gaactcccga tgaaaccgga agacatcgac
     1261 atgaatcctg aggctctcac cgcgtggaaa cgtgctgccg ctgctgtgta ccgcaaggac
     1321 aaggctcgca agtctcgccg tatcagcctt gagttcatgc ttgagcaagc caataagttt
     1381 gctaaccata aggccatctg gttcccttac aacatggact ggcgcggtcg tgtttacgct
     1441 gtgtcaatgt tcaacccgca aggtaacgat atgaccaaag gactgcttac gctggcgaaa
     1501 ggtaaaccaa tcggtaagga aggttactac tggctgaaaa tccacggtgc aaactgtgcg
     1561 ggtgtcgata aggttccgtt ccctgagcgc atcaagttca ttgaggaaaa ccacgagaac
     1621 atcatggctt gcgctaagtc tccactggag aacacttggt gggctgagca agattctccg
     1681 ttctgcttcc ttgcgttctg ctttgagtac gctggggtac agcaccacgg cctgagctat
     1741 aactgctccc ttccgctggc gtttgacggg tcttgctctg gcatccagca cttctccgcg
     1801 atgctccgag atgaggtagg tggtcgcgcg gttaacttgc ttcctagtga aaccgttcag
     1861 gacatctacg ggattgttgc taagaaagtc aacgagattc tacaagcaga cgcaatcaat
     1921 gggaccgata acgaagtagt taccgtgacc gatgagaaca ctggtgaaat ctctgagaaa
     1981 gtcaagctgg gcactaaggc actggctggt caatggctgg cttacggtgt tactcgcagt
     2041 gtgactaagc gttcagtcat gacgctggct tacgggtcca aagagttcgg cttccgtcaa
     2101 caagtgctgg aagataccat tcagccagct attgattccg gcaagggtct gatgttcact
     2161 cagccgaatc aggctgctgg atacatggct aagctgattt gggaatctgt gagcgtgacg
     2221 gtggtagctg cggttgaagc aatgaactgg cttaagtctg ctgctaagct gctggctgct
     2281 gaggtcaaag ataagaagac tggagagatt cttcgcaagc gttgcgctgt gcattgggta
     2341 actcctgatg gtttccctgt gtggcaggaa tacaagaagc ctattcagac gcgcttgaac
     2401 ctgatgttcc tcggtcagtt ccgcttacag cctaccatta acaccaacaa agatagcgag
     2461 attgatgcac acaaacagga gtctggtatc gctcctaact ttgtacacag ccaagacggt
     2521 agccaccttc gtaagactgt agtgtgggca cacgagaagt acggaatcga atcttttgca
     2581 ctgattcacg actccttcgg taccattccg gctgacgctg cgaacctgtt caaagcagtg
     2641 cgcgaaacta tggttgacac atatgagtct tgtgatgtac tggctgattt ctacgaccag
     2701 ttcgctgacc agttgcacga gtctcaattg gacaaaatgc cagcacttcc ggctaaaggt
     2761 aacttgaacc tccgtgacat cttagagtcg gacttcgcgt tcgcgtaata atactagagc
     2821 caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg
     2881 tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg
     2941 cgtttata
//