LOCUS BBa_K1450012 5367 bp DNA linear 08-May-2024 DEFINITION T7 RNA Polymerase and DNA Polymerase ACCESSION BBa_K1450012 VERSION BBa_K1450012.1 FEATURES Location/Qualifiers promoter 1..130 /label=BBa_I13453 misc_feature 143..146 /label=conserved RBS 139..150 /label=BBa_B0034 CDS 157..2811 /label=BBa_K1450001 stem_loop 2831..2874 /label=stem_loop misc_feature 2820..2899 /label=BBa_B0010 terminator 2820..2899 /label=BBa_B0010 stem_loop 2915..2934 /label=T7 TE misc_feature 2941..2941 /label=stop polyA_site 2935..2948 /label=polya misc_feature 2908..2948 /label=BBa_B0012 terminator 2908..2948 /label=BBa_B0012 terminator 2820..2948 /label=BBa_B0015 promoter 2957..3086 /label=BBa_I13453 misc_feature 3099..3102 /label=conserved RBS 3095..3106 /label=BBa_B0034 CDS 3113..5230 /label=BBa_K1450000 stem_loop 5250..5293 /label=stem_loop misc_feature 5239..5318 /label=BBa_B0010 terminator 5239..5318 /label=BBa_B0010 stem_loop 5334..5353 /label=T7 TE misc_feature 5360..5360 /label=stop polyA_site 5354..5367 /label=polya misc_feature 5327..5367 /label=BBa_B0012 terminator 5327..5367 /label=BBa_B0012 terminator 5239..5367 /label=BBa_B0015 ORIGIN 1 acattgatta tttgcacggc gtcacacttt gctatgccat agcattttta tccataagat 61 tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca taccgttttt 121 ttgggctagc tactagagaa agaggagaaa tactagatga acacgattaa catcgctaag 181 aacgacttct ctgacatcga actggctgct atcccgttca acactctggc tgaccattac 241 ggtgagcgtt tagctcgcga acagttggcc cttgagcatg agtcttacga gatgggtgaa 301 gcacgcttcc gcaagatgtt tgagcgtcaa cttaaagctg gtgaggttgc ggataacgct 361 gccgccaagc ctctcatcac taccctactc cctaagatga ttgcacgcat caacgactgg 421 tttgaggaag tgaaagctaa gcgcggcaag cgcccgacag ccttccagtt cctgcaagaa 481 atcaagccgg aagccgtagc gtacatcacc attaagacca ctctggcttg cctaaccagt 541 gctgacaata caaccgttca ggctgtagca agcgcaatcg gtcgggccat tgaggacgag 601 gctcgcttcg gtcgtatccg tgaccttgaa gctaagcact tcaagaaaaa cgttgaggaa 661 caactcaaca agcgcgtagg gcacgtctac aagaaagcat ttatgcaagt tgtcgaggct 721 gacatgctct ctaagggtct actcggtggc gaggcgtggt cttcgtggca taaggaagac 781 tctattcatg taggagtacg ctgcatcgag atgctcattg agtcaaccgg aatggttagc 841 ttacaccgcc aaaatgctgg cgtagtaggt caagactctg agactatcga actcgcacct 901 gaatacgctg aggctatcgc aacccgtgca ggtgcgctgg ctggcatctc tccgatgttc 961 caaccttgcg tagttcctcc taagccgtgg actggcatta ctggtggtgg ctattgggct 1021 aacggtcgtc gtcctctggc gctggtgcgt actcacagta agaaagcact gatgcgctac 1081 gaagacgttt acatgcctga ggtgtacaaa gcgattaaca ttgcgcaaaa caccgcatgg 1141 aaaatcaaca agaaagtcct agcggtcgcc aacgtaatca ccaagtggaa gcattgtccg 1201 gtcgaggaca tccctgcgat tgagcgtgaa gaactcccga tgaaaccgga agacatcgac 1261 atgaatcctg aggctctcac cgcgtggaaa cgtgctgccg ctgctgtgta ccgcaaggac 1321 aaggctcgca agtctcgccg tatcagcctt gagttcatgc ttgagcaagc caataagttt 1381 gctaaccata aggccatctg gttcccttac aacatggact ggcgcggtcg tgtttacgct 1441 gtgtcaatgt tcaacccgca aggtaacgat atgaccaaag gactgcttac gctggcgaaa 1501 ggtaaaccaa tcggtaagga aggttactac tggctgaaaa tccacggtgc aaactgtgcg 1561 ggtgtcgata aggttccgtt ccctgagcgc atcaagttca ttgaggaaaa ccacgagaac 1621 atcatggctt gcgctaagtc tccactggag aacacttggt gggctgagca agattctccg 1681 ttctgcttcc ttgcgttctg ctttgagtac gctggggtac agcaccacgg cctgagctat 1741 aactgctccc ttccgctggc gtttgacggg tcttgctctg gcatccagca cttctccgcg 1801 atgctccgag atgaggtagg tggtcgcgcg gttaacttgc ttcctagtga aaccgttcag 1861 gacatctacg ggattgttgc taagaaagtc aacgagattc tacaagcaga cgcaatcaat 1921 gggaccgata acgaagtagt taccgtgacc gatgagaaca ctggtgaaat ctctgagaaa 1981 gtcaagctgg gcactaaggc actggctggt caatggctgg cttacggtgt tactcgcagt 2041 gtgactaagc gttcagtcat gacgctggct tacgggtcca aagagttcgg cttccgtcaa 2101 caagtgctgg aagataccat tcagccagct attgattccg gcaagggtct gatgttcact 2161 cagccgaatc aggctgctgg atacatggct aagctgattt gggaatctgt gagcgtgacg 2221 gtggtagctg cggttgaagc aatgaactgg cttaagtctg ctgctaagct gctggctgct 2281 gaggtcaaag ataagaagac tggagagatt cttcgcaagc gttgcgctgt gcattgggta 2341 actcctgatg gtttccctgt gtggcaggaa tacaagaagc ctattcagac gcgcttgaac 2401 ctgatgttcc tcggtcagtt ccgcttacag cctaccatta acaccaacaa agatagcgag 2461 attgatgcac acaaacagga gtctggtatc gctcctaact ttgtacacag ccaagacggt 2521 agccaccttc gtaagactgt agtgtgggca cacgagaagt acggaatcga atcttttgca 2581 ctgattcacg actccttcgg taccattccg gctgacgctg cgaacctgtt caaagcagtg 2641 cgcgaaacta tggttgacac atatgagtct tgtgatgtac tggctgattt ctacgaccag 2701 ttcgctgacc agttgcacga gtctcaattg gacaaaatgc cagcacttcc ggctaaaggt 2761 aacttgaacc tccgtgacat cttagagtcg gacttcgcgt tcgcgtaata atactagagc 2821 caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg 2881 tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg 2941 cgtttatata ctagagacat tgattatttg cacggcgtca cactttgcta tgccatagca 3001 tttttatcca taagattagc ggatcctacc tgacgctttt tatcgcaact ctctactgtt 3061 tctccatacc gtttttttgg gctagctact agagaaagag gagaaatact agatgatcgt 3121 ttctgacatc gaagctaacg ccctcttaga gagcgtcact aagttccact gcggggttat 3181 ctacgactac tccaccgctg agtacgtaag ctaccgtccg agtgacttcg gtgcgtatct 3241 ggatgcgctg gaagccgagg ttgcacgagg cggtcttatt gtgttccaca acggtcacaa 3301 gtatgacgtt cctgcattga ccaaactggc aaagttgcaa ttgaaccgag agttccacct 3361 tcctcgtgag aactgtattg acacccttgt gttgtcacgt ttgattcatt ccaacctcaa 3421 ggacaccgat atgggtcttc tgcgttccgg caagttgccc ggaaaacgct ttgggtctca 3481 cgctttggag gcgtggggtt atcgcttagg cgagatgaag ggtgaataca aagacgactt 3541 taagcgtatg cttgaagagc agggtgaaga atacgttgac ggaatggagt ggtggaactt 3601 caacgaagag atgatggact ataacgttca ggacgttgtg gtaactaaag ctctccttga 3661 gaagctactc tctgacaaac attacttccc tcctgagatt gactttacgg acgtaggata 3721 cactacgttc tggtcagaat cccttgaggc cgttgacatt gaacatcgtg ctgcatggct 3781 gctcgctaaa caagagcgca acgggttccc gtttgacaca aaagcaatcg aagagttgta 3841 cgtagagtta gctgctcgcc gctctgagtt gctccgtaaa ttgaccgaaa cgttcggctc 3901 gtggtatcag cctaaaggtg gcactgagat gttctgccat ccgcgaacag gtaagccact 3961 acctaaatac cctcgcatta agacacctaa agttggtggt atctttaaga agcctaagaa 4021 caaggcacag cgagaaggcc gtgagccttg cgaacttgat acccgcgagt acgttgctgg 4081 tgctccttac accccagttg aacatgttgt gtttaaccct tcgtctcgtg accacattca 4141 gaagaaactc caagaggctg ggtgggtccc gaccaagtac accgataagg gtgctcctgt 4201 ggtggacgat gaggtactcg aaggagtacg tgtagatgac cctgagaagc aagccgctat 4261 cgacctcatt aaagagtact tgatgattca gaagcgaatc ggacagtctg ctgagggaga 4321 caaagcatgg cttcgttatg ttgctgagga tggtaagatt catggttctg ttaaccctaa 4381 tggagcagtt acgggtcgtg cgacccatgc gttcccaaac cttgcgcaaa ttccgggtgt 4441 acgttctcct tatggagagc agtgtcgcgc tgcttttggc gctgagcacc atttggatgg 4501 gataactggt aagccttggg ttcaggctgg catcgacgca tccggtcttg agctacgctg 4561 cttggctcac ttcatggctc gctttgataa cggcgagtac gctcacgaga ttcttaacgg 4621 cgacatccac actaagaacc agatagctgc tgaactacct acccgagata acgctaagac 4681 gttcatctat gggttcctct atggtgctgg tgatgagaag attggacaga ttgttggtgc 4741 tggtaaagag cgcggtaagg aactcaagaa gaaattcctt gagaacaccc ccgcgattgc 4801 agcactccgc gagtctatcc aacagacact tgtcgagtcc tctcaatggg tagctggtga 4861 gcaacaagtc aagtggaaac gccgctggat taaaggtctg gatggtcgta aggtacacgt 4921 tcgtagtcct cacgctgcct tgaataccct actgcaatct gctggtgctc tcatctgcaa 4981 actgtggatt atcaagaccg aagagatgct cgtagagaaa ggcttgaagc atggctggga 5041 tggggacttt gcgtacatgg catgggtaca tgatgaaatc caagtaggct gccgtaccga 5101 agagattgct caggtggtca ttgagaccgc acaagaagcg atgcgctggg ttggagacca 5161 ctggaacttc cggtgtcttc tggataccga aggtaagatg ggtcctaatt gggcgatttg 5221 ccactgataa tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg 5281 cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac 5341 cttcgggtgg gcctttctgc gtttata //