LOCUS       BBa_K145004             2262 bp    DNA     linear       12-May-2024
DEFINITION  T7 PoPS + RiboLock |> LuxI
ACCESSION   BBa_K145004
VERSION     BBa_K145004.1  
FEATURES             Location/Qualifiers
     promoter        1..46
                     /label=BBa_I712074
     misc_feature    55..97
                     /label=BBa_J23032
     misc_feature    1193..1231
                     /label=LVA
     CDS             104..1231
                     /label=lacI-LVA
     misc_feature    104..1231
                     /label=BBa_C0012
     misc_feature    1232..1256
                     /label=Help:Barcodes
     CDS             104..1231
                     /label=BBa_C0012
     stem_loop       1276..1319
                     /label=stem_loop
     misc_feature    1265..1344
                     /label=BBa_B0010
     terminator      1265..1344
                     /label=BBa_B0010
     stem_loop       1360..1379
                     /label=T7 TE
     misc_feature    1386..1386
                     /label=stop
     polyA_site      1380..1393
                     /label=polya
     misc_feature    1353..1393
                     /label=BBa_B0012
     terminator      1353..1393
                     /label=BBa_B0012
     terminator      1265..1393
                     /label=BBa_B0015
     misc_feature    1404..1420
                     /label=lac O1
     promoter        1421..1426
                     /label=-35
     misc_feature    1427..1443
                     /label=lac O1
     promoter        1444..1449
                     /label=-10
     misc_feature    1402..1455
                     /label=BBa_R0011
     promoter        1402..1455
                     /label=BBa_R0011
     misc_feature    1469..1472
                     /label=conserved
     RBS             1465..1476
                     /label=BBa_B0034
     misc_feature    2062..2093
                     /label=LVA
     CDS             1483..2061
                     /label=luxI
     misc_feature    1483..2100
                     /label=BBa_C0061
     misc_feature    2101..2125
                     /label=Help:Barcodes
     CDS             1483..2100
                     /label=BBa_C0061
     stem_loop       2145..2188
                     /label=stem_loop
     misc_feature    2134..2213
                     /label=BBa_B0010
     terminator      2134..2213
                     /label=BBa_B0010
     stem_loop       2229..2248
                     /label=T7 TE
     misc_feature    2255..2255
                     /label=stop
     polyA_site      2249..2262
                     /label=polya
     misc_feature    2222..2262
                     /label=BBa_B0012
     terminator      2222..2262
                     /label=BBa_B0012
     misc_feature    1465..2262
                     /label=BBa_F1610
ORIGIN
        1 taatacgact cactataggg aatacaagct acttgttctt tttgcatact agagaactag
       61 aatcacctct tgcttttggg taagacagaa gaggagatac tagatggtga atgtgaaacc
      121 agtaacgtta tacgatgtcg cagagtatgc cggtgtctct tatcagaccg tttcccgcgt
      181 ggtgaaccag gccagccacg tttctgcgaa aacgcgggaa aaagtggaag cggcgatggc
      241 ggagctgaat tacattccca accgcgtggc acaacaactg gcgggcaaac agtcgttgct
      301 gattggcgtt gccacctcca gtctggccct gcacgcgccg tcgcaaattg tcgcggcgat
      361 taaatctcgc gccgatcaac tgggtgccag cgtggtggtg tcgatggtag aacgaagcgg
      421 cgtcgaagcc tgtaaagcgg cggtgcacaa tcttctcgcg caacgcgtca gtgggctgat
      481 cattaactat ccgctggatg accaggatgc cattgctgtg gaagctgcct gcactaatgt
      541 tccggcgtta tttcttgatg tctctgacca gacacccatc aacagtatta ttttctccca
      601 tgaagacggt acgcgactgg gcgtggagca tctggtcgca ttgggtcacc agcaaatcgc
      661 gctgttagcg ggcccattaa gttctgtctc ggcgcgtctg cgtctggctg gctggcataa
      721 atatctcact cgcaatcaaa ttcagccgat agcggaacgg gaaggcgact ggagtgccat
      781 gtccggtttt caacaaacca tgcaaatgct gaatgagggc atcgttccca ctgcgatgct
      841 ggttgccaac gatcagatgg cgctgggcgc aatgcgcgcc attaccgagt ccgggctgcg
      901 cgttggtgcg gatatctcgg tagtgggata cgacgatacc gaagacagct catgttatat
      961 cccgccgtta accaccatca aacaggattt tcgcctgctg gggcaaacca gcgtggaccg
     1021 cttgctgcaa ctctctcagg gccaggcggt gaagggcaat cagctgttgc ccgtctcact
     1081 ggtgaaaaga aaaaccaccc tggcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     1141 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc aggctgcaaa
     1201 cgacgaaaac tacgctttag tagcttaata actctgatag tgctagtgta gatctctact
     1261 agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc
     1321 tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct
     1381 ttctgcgttt atatactaga gaattgtgag cggataacaa ttgacattgt gagcggataa
     1441 caagatactg agcacatact agagaaagag gagaaatact agatgactat aatgataaaa
     1501 aaatcggatt ttttggcaat tccatcggag gagtataaag gtattctaag tcttcgttat
     1561 caagtgttta agcaaagact tgagtgggac ttagttgtag aaaataacct tgaatcagat
     1621 gagtatgata actcaaatgc agaatatatt tatgcttgtg atgatactga aaatgtaagt
     1681 ggatgctggc gtttattacc tacaacaggt gattatatgc tgaaaagtgt ttttcctgaa
     1741 ttgcttggtc aacagagtgc tcccaaagat cctaatatag tcgaattaag tcgttttgct
     1801 gtaggtaaaa atagctcaaa gataaataac tctgctagtg aaattacaat gaaactattt
     1861 gaagctatat ataaacacgc tgttagtcaa ggtattacag aatatgtaac agtaacatca
     1921 acagcaatag agcgattttt aaagcgtatt aaagttcctt gtcatcgtat tggagacaaa
     1981 gaaattcatg tattaggtga tactaaatcg gttgtattgt ctatgcctat taatgaacag
     2041 tttaaaaaag cagtcttaaa tgctgcaaac gacgaaaact acgctttagt agcttaataa
     2101 ctctgatagt gctagtgtag atctctacta gagccaggca tcaaataaaa cgaaaggctc
     2161 agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga
     2221 gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta ta
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