##gff-version 3
##sequence-region BBa_K145004 1 2262
BBa_K145004	.	engineered_region	1465	2262	.	+	0	ID=BBa_F1610;Name=BBa_F1610
BBa_K145004	.	terminator	2134	2213	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_F1610
BBa_K145004	.	stem_loop	2145	2188	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K145004	.	engineered_region	2134	2213	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K145004	.	ribosome_entry_site	1465	1476	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_F1610
BBa_K145004	.	conserved_region	1469	1472	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K145004	.	terminator	2222	2262	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_F1610
BBa_K145004	.	polyA_site	2249	2262	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K145004	.	stop_codon	2255	2255	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K145004	.	stem_loop	2229	2248	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K145004	.	engineered_region	2222	2262	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K145004	.	CDS	1483	2100	.	+	0	ID=BBa_C0061;Name=BBa_C0061;Parent=BBa_F1610
BBa_K145004	.	tag	2062	2093	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_K145004	.	engineered_region	1483	2100	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_K145004	.	engineered_tag	2101	2125	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_K145004	.	CDS	1483	2061	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_K145004	.	promoter	1402	1455	.	+	0	ID=BBa_R0011;Name=BBa_R0011
BBa_K145004	.	promoter	1444	1449	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_K145004	.	operator	1427	1443	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_K145004	.	engineered_region	1402	1455	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_K145004	.	operator	1404	1420	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_K145004	.	promoter	1421	1426	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_K145004	.	CDS	104	1231	.	+	0	ID=BBa_C0012;Name=BBa_C0012
BBa_K145004	.	engineered_tag	1232	1256	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_K145004	.	CDS	104	1231	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_K145004	.	engineered_region	104	1231	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_K145004	.	tag	1193	1231	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_K145004	.	terminator	1265	1393	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K145004	.	terminator	1353	1393	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K145004	.	polyA_site	1380	1393	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K145004	.	stop_codon	1386	1386	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K145004	.	stem_loop	1360	1379	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K145004	.	engineered_region	1353	1393	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K145004	.	terminator	1265	1344	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K145004	.	stem_loop	1276	1319	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K145004	.	engineered_region	1265	1344	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K145004	.	promoter	1	46	.	+	0	ID=BBa_I712074;Name=BBa_I712074
BBa_K145004	.	mature_transcript_region	55	97	.	+	0	ID=BBa_J23032;Name=BBa_J23032
>BBa_K145004
taatacgactcactatagggaatacaagctacttgttctttttgcatactagagaactagaatcacctcttgcttttgg
gtaagacagaagaggagatactagatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtct
cttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgat
ggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacc
tccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtgg
tggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgg
gctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttattt
cttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatc
tggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctgg
ctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggtttt
caacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcg
caatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacag
ctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctg
caactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgc
ccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaag
cgggcaggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagag
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctct
ctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagaattgtgagcggataacaatt
gacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatactagatgactataatgataaaaa
aatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagact
tgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgt
gatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctg
aattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctc
aaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtatt
acagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggag
acaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagt
cttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagag
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctct
ctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata