LOCUS       BBa_K145005             1088 bp    DNA     linear       06-May-2024
DEFINITION  T7 PoPS + P<sub>R</sub> -> cI
ACCESSION   BBa_K145005
VERSION     BBa_K145005.1  
FEATURES             Location/Qualifiers
     promoter        1..46
                     /label=BBa_I712074
     promoter        92..97
                     /label=-10
     promoter        69..74
                     /label=-35
     misc_feature    79..95
                     /label=OR1
     misc_feature    55..71
                     /label=OR2
     misc_feature    55..103
                     /label=BBa_R0051
     promoter        55..103
                     /label=BBa_R0051
     misc_feature    112..154
                     /label=BBa_J23032
     misc_feature    169..172
                     /label=conserved
     RBS             163..176
                     /label=BBa_B0031
     misc_feature    921..926
                     /label=2
     CDS             183..926
                     /label=cI HK022
     misc_feature    888..920
                     /label=SsrA
     misc_feature    183..190
                     /label=OR3 partial
     misc_feature    183..926
                     /label=BBa_C0050
     misc_feature    927..951
                     /label=Help:Barcodes
     CDS             183..926
                     /label=BBa_C0050
     stem_loop       971..1014
                     /label=stem_loop
     misc_feature    960..1039
                     /label=BBa_B0010
     terminator      960..1039
                     /label=BBa_B0010
     stem_loop       1055..1074
                     /label=T7 TE
     misc_feature    1081..1081
                     /label=stop
     polyA_site      1075..1088
                     /label=polya
     misc_feature    1048..1088
                     /label=BBa_B0012
     terminator      1048..1088
                     /label=BBa_B0012
     misc_feature    163..1088
                     /label=BBa_P0150
ORIGIN
        1 taatacgact cactataggg aatacaagct acttgttctt tttgcatact agagtaacac
       61 cgtgcgtgtt gactatttta cctctggcgg tgataatggt tgctactaga gaactagaat
      121 cacctcttgc ttttgggtaa gacagaagag gagatactag agtcacacag gaaacctact
      181 agatggttca acagaaagag cgtgaaactt tctcgcagag gcttgcgctg gcctgtgata
      241 aagcgggatt acctttgcat ggtaggcagg ctgatttagc tgtcaggctt aaggtcacac
      301 caaaagccat tagtaaatgg ttcaacgggg agtcaatacc aagaaaagac aagatggaat
      361 ctctggcttc ggtgctggga actactgctg catatctgca tggctatgct gatgatgacg
      421 gtatcacggt aaatcatcta tcaagatcaa atgattatta tcgtgttgat gtattggatg
      481 ttcaggcgag cgccgggcca ggaaccatgg tttccaatga atttatagaa aagataagag
      541 caattgaata tacgaccgag caggcaagaa ttttatttaa tggaaggcca caggaaagcg
      601 taaaagtcat cacggttcgc ggtgacagca tggagggaac catcaatccg ggagatgaga
      661 tctttgttga tgtatccata acctgttttg atggcgatgg catttatgtg tttgtatacg
      721 ggaaaacaat gcacgttaag cgcctgcaaa tgcaaaagaa caggcttgcc gtcatctctg
      781 acaatgccgc ttatgatcga tggtacatag aagaaggtga agaagagcaa cttcacattc
      841 tagccaaagt cctcattagg cagtcaatcg attacaagcg attcggagct gcaaacgacg
      901 aaaactacgc tttagtagct taataaccct gatagtgcta gtgtagatcc ctactagagc
      961 caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg
     1021 tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg
     1081 cgtttata
//