LOCUS       BBa_K145006             1059 bp    DNA     linear       13-May-2024
DEFINITION  Lactonase under P<sub>RM</sub> control
ACCESSION   BBa_K145006
VERSION     BBa_K145006.1  
FEATURES             Location/Qualifiers
     misc_feature    9..25
                     /label=OR1
     misc_feature    33..49
                     /label=OR2
     promoter        48..53
                     /label=-35
     misc_feature    59..65
                     /label=mutate to obliterate OR3
     promoter        71..76
                     /label=-10
     promoter        1..82
                     /label=BBa_I12007
     misc_feature    95..98
                     /label=conserved
     RBS             91..102
                     /label=BBa_B0034
     misc_feature    109..111
                     /label=start
     misc_feature    892..897
                     /label=2
     misc_feature    859..891
                     /label=LVA
     CDS             109..858
                     /label=aiiA
     misc_feature    109..897
                     /label=BBa_C0060
     misc_feature    898..922
                     /label=Help:Barcodes
     CDS             109..897
                     /label=BBa_C0060
     stem_loop       942..985
                     /label=stem_loop
     misc_feature    931..1010
                     /label=BBa_B0010
     terminator      931..1010
                     /label=BBa_B0010
     stem_loop       1026..1045
                     /label=T7 TE
     misc_feature    1052..1052
                     /label=stop
     polyA_site      1046..1059
                     /label=polya
     misc_feature    1019..1059
                     /label=BBa_B0012
     terminator      1019..1059
                     /label=BBa_B0012
     terminator      931..1059
                     /label=BBa_B0015
ORIGIN
        1 gcaaccatta tcaccgccag aggtaaaata gtcaacacgc acggtgttag atatttataa
       61 atagtggtga tagatttaac gttactagag aaagaggaga aatactagat gacagtaaag
      121 aagctttatt tcgtcccagc aggtcgttgt atgttggatc attcgtctgt taatagtaca
      181 ttaacaccag gagaattatt agacttaccg gtttggtgtt atcttttgga gactgaagaa
      241 ggacctattt tagtagatac aggtatgcca gaaagtgcag ttaataatga aggtcttttt
      301 aacggtacat ttgtcgaagg gcaggtttta ccgaaaatga ctgaagaaga tagaatcgtg
      361 aatattttaa aacgggttgg ttatgagccg gaagaccttc tttatattat tagttctcac
      421 ttgcattttg atcatgcagg aggaaatggc gcttttataa atacaccaat cattgtacag
      481 cgtgctgaat atgaggcggc gcagcatagc gaagaatatt tgaaagaatg tatattgccg
      541 aatttaaact acaaaatcat tgaaggtgat tatgaagtcg taccaggagt tcaattattg
      601 catacaccag gccatactcc agggcatcaa tcgctattaa ttgagacaga aaaatccggt
      661 cctgtattat taacgattga tgcatcgtat acgaaagaga attttgaaaa tgaagtgcca
      721 tttgcgggat ttgattcaga attagcttta tcttcaatta aacgtttaaa agaagtggtg
      781 atgaaagaga agccgattgt tttctttgga catgatatag agcaggaaag gggatgtaaa
      841 gtgttccctg aatatatagc tgcaaacgac gaaaactacg ctttagtagc ttaataacgc
      901 tgatagtgct agtgtagatc gctactagag ccaggcatca aataaaacga aaggctcagt
      961 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc
     1021 acactggctc accttcgggt gggcctttct gcgtttata
//