LOCUS       BBa_K145007             2155 bp    DNA     linear       23-Nov-2024
DEFINITION  Pulse Generator
ACCESSION   BBa_K145007
VERSION     BBa_K145007.1  
FEATURES             Location/Qualifiers
     promoter        1..46
                     /label=BBa_I712074
     promoter        92..97
                     /label=-10
     promoter        69..74
                     /label=-35
     misc_feature    79..95
                     /label=OR1
     misc_feature    55..71
                     /label=OR2
     misc_feature    55..103
                     /label=BBa_R0051
     promoter        55..103
                     /label=BBa_R0051
     misc_feature    112..154
                     /label=BBa_J23032
     misc_feature    169..172
                     /label=conserved
     RBS             163..176
                     /label=BBa_B0031
     misc_feature    921..926
                     /label=2
     CDS             183..926
                     /label=cI HK022
     misc_feature    888..920
                     /label=SsrA
     misc_feature    183..190
                     /label=OR3 partial
     misc_feature    183..926
                     /label=BBa_C0050
     misc_feature    927..951
                     /label=Help:Barcodes
     CDS             183..926
                     /label=BBa_C0050
     stem_loop       971..1014
                     /label=stem_loop
     misc_feature    960..1039
                     /label=BBa_B0010
     terminator      960..1039
                     /label=BBa_B0010
     stem_loop       1055..1074
                     /label=T7 TE
     misc_feature    1081..1081
                     /label=stop
     polyA_site      1075..1088
                     /label=polya
     misc_feature    1048..1088
                     /label=BBa_B0012
     terminator      1048..1088
                     /label=BBa_B0012
     misc_feature    163..1088
                     /label=BBa_P0150
     misc_feature    1..1088
                     /label=BBa_K145005
     misc_feature    1105..1121
                     /label=OR1
     misc_feature    1129..1145
                     /label=OR2
     promoter        1144..1149
                     /label=-35
     misc_feature    1155..1161
                     /label=mutate to obliterate OR3
     promoter        1167..1172
                     /label=-10
     promoter        1097..1178
                     /label=BBa_I12007
     misc_feature    1191..1194
                     /label=conserved
     RBS             1187..1198
                     /label=BBa_B0034
     misc_feature    1205..1207
                     /label=start
     misc_feature    1988..1993
                     /label=2
     misc_feature    1955..1987
                     /label=LVA
     CDS             1205..1954
                     /label=aiiA
     misc_feature    1205..1993
                     /label=BBa_C0060
     misc_feature    1994..2018
                     /label=Help:Barcodes
     CDS             1205..1993
                     /label=BBa_C0060
     stem_loop       2038..2081
                     /label=stem_loop
     misc_feature    2027..2106
                     /label=BBa_B0010
     terminator      2027..2106
                     /label=BBa_B0010
     stem_loop       2122..2141
                     /label=T7 TE
     misc_feature    2148..2148
                     /label=stop
     polyA_site      2142..2155
                     /label=polya
     misc_feature    2115..2155
                     /label=BBa_B0012
     terminator      2115..2155
                     /label=BBa_B0012
     terminator      2027..2155
                     /label=BBa_B0015
     misc_feature    1097..2155
                     /label=BBa_K145006
ORIGIN
        1 taatacgact cactataggg aatacaagct acttgttctt tttgcatact agagtaacac
       61 cgtgcgtgtt gactatttta cctctggcgg tgataatggt tgctactaga gaactagaat
      121 cacctcttgc ttttgggtaa gacagaagag gagatactag agtcacacag gaaacctact
      181 agatggttca acagaaagag cgtgaaactt tctcgcagag gcttgcgctg gcctgtgata
      241 aagcgggatt acctttgcat ggtaggcagg ctgatttagc tgtcaggctt aaggtcacac
      301 caaaagccat tagtaaatgg ttcaacgggg agtcaatacc aagaaaagac aagatggaat
      361 ctctggcttc ggtgctggga actactgctg catatctgca tggctatgct gatgatgacg
      421 gtatcacggt aaatcatcta tcaagatcaa atgattatta tcgtgttgat gtattggatg
      481 ttcaggcgag cgccgggcca ggaaccatgg tttccaatga atttatagaa aagataagag
      541 caattgaata tacgaccgag caggcaagaa ttttatttaa tggaaggcca caggaaagcg
      601 taaaagtcat cacggttcgc ggtgacagca tggagggaac catcaatccg ggagatgaga
      661 tctttgttga tgtatccata acctgttttg atggcgatgg catttatgtg tttgtatacg
      721 ggaaaacaat gcacgttaag cgcctgcaaa tgcaaaagaa caggcttgcc gtcatctctg
      781 acaatgccgc ttatgatcga tggtacatag aagaaggtga agaagagcaa cttcacattc
      841 tagccaaagt cctcattagg cagtcaatcg attacaagcg attcggagct gcaaacgacg
      901 aaaactacgc tttagtagct taataaccct gatagtgcta gtgtagatcc ctactagagc
      961 caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg
     1021 tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg
     1081 cgtttatata ctagaggcaa ccattatcac cgccagaggt aaaatagtca acacgcacgg
     1141 tgttagatat ttataaatag tggtgataga tttaacgtta ctagagaaag aggagaaata
     1201 ctagatgaca gtaaagaagc tttatttcgt cccagcaggt cgttgtatgt tggatcattc
     1261 gtctgttaat agtacattaa caccaggaga attattagac ttaccggttt ggtgttatct
     1321 tttggagact gaagaaggac ctattttagt agatacaggt atgccagaaa gtgcagttaa
     1381 taatgaaggt ctttttaacg gtacatttgt cgaagggcag gttttaccga aaatgactga
     1441 agaagataga atcgtgaata ttttaaaacg ggttggttat gagccggaag accttcttta
     1501 tattattagt tctcacttgc attttgatca tgcaggagga aatggcgctt ttataaatac
     1561 accaatcatt gtacagcgtg ctgaatatga ggcggcgcag catagcgaag aatatttgaa
     1621 agaatgtata ttgccgaatt taaactacaa aatcattgaa ggtgattatg aagtcgtacc
     1681 aggagttcaa ttattgcata caccaggcca tactccaggg catcaatcgc tattaattga
     1741 gacagaaaaa tccggtcctg tattattaac gattgatgca tcgtatacga aagagaattt
     1801 tgaaaatgaa gtgccatttg cgggatttga ttcagaatta gctttatctt caattaaacg
     1861 tttaaaagaa gtggtgatga aagagaagcc gattgttttc tttggacatg atatagagca
     1921 ggaaagggga tgtaaagtgt tccctgaata tatagctgca aacgacgaaa actacgcttt
     1981 agtagcttaa taacgctgat agtgctagtg tagatcgcta ctagagccag gcatcaaata
     2041 aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac
     2101 gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttata
//