LOCUS BBa_K145009 895 bp DNA linear 25-Nov-2024 DEFINITION Lux PR PoPS -> ccdB ACCESSION BBa_K145009 VERSION BBa_K145009.1 FEATURES Location/Qualifiers misc_feature 1..20 /label=LuxR/HSL promoter 20..25 /label=-35 promoter 42..47 /label=-10 misc_feature 53..53 /label=start misc_feature 1..55 /label=BBa_R0062 promoter 1..55 /label=BBa_R0062 misc_feature 68..71 /label=conserved RBS 64..75 /label=BBa_B0034 CDS 112..417 /label=ccdB (rev) CDS 419..639 /label=ccdA- (rev) misc_feature 550..550 /label=T to C mutation misc_feature 678..683 /label=-10 (rev) misc_feature 701..706 /label=-35 (rev) misc_feature 84..758 /label=BBa_P1010 stem_loop 778..821 /label=stem_loop misc_feature 767..846 /label=BBa_B0010 terminator 767..846 /label=BBa_B0010 stem_loop 862..881 /label=T7 TE misc_feature 888..888 /label=stop polyA_site 882..895 /label=polya misc_feature 855..895 /label=BBa_B0012 terminator 855..895 /label=BBa_B0012 ORIGIN 1 acctgtagga tcgtacaggt ttacgcaaga aaatggtttg ttatagtcga ataaatacta 61 gagaaagagg agaaatacta gagactggct gtgtataagg gagcctgaca tttatattcc 121 ccagaacatc aggttaatgg cgtttttgat gtcattttcg cggtggctga gatcagccac 181 ttcttccccg ataacggaga ccggcacact ggccatatcg gtggtcatca tgcgccagct 241 ttcatccccg atatgcacca ccgggtaaag ttcacgggag actttatctg acagcagacg 301 tgcactggcc agggggatca ccatccgtcg cccgggcgtg tcaataatat cactctgtac 361 atccacaaac agacgataac ggctctctct tttataggtg taaaccttaa actgcatttc 421 accagcccct gttctcgtca gcaaaagagc cgttcatttc aataaaccgg gcgacctcag 481 ccatcccttc ctgattttcc gctttccagc gttcggcacg cagacgacgg gcttcattct 541 gcatggttgt gcttaccaga ccggagatat tgacatcata tatgccttga gcaactgata 601 gctgtcgctg tcaactgtca ctgtaatacg ctgcttcata gcatacctct ttttgacata 661 cttcgggtat acatatcagt atatattctt ataccgcaaa aatcagcgcg caaatacgca 721 tactgttatc tggcttttag taagccggat ccacgcgtta ctagagccag gcatcaaata 781 aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac 841 gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttata //