##gff-version 3
##sequence-region BBa_K145009 1 895
BBa_K145009	.	ribosome_entry_site	64	75	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K145009	.	conserved_region	68	71	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K145009	.	promoter	1	55	.	+	0	ID=BBa_R0062;Name=BBa_R0062
BBa_K145009	.	promoter	20	25	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_K145009	.	engineered_region	1	55	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_K145009	.	promoter	42	47	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_K145009	.	start_codon	53	53	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_K145009	.	non_covalent_binding_site	1	20	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_K145009	.	engineered_region	84	758	.	+	0	ID=BBa_P1010;Name=BBa_P1010
BBa_K145009	.	CDS	419	639	.	+	0	ID=annotation1747333;Name=ccdA- (rev);Parent=BBa_P1010
BBa_K145009	.	sequence_feature	701	706	.	+	0	ID=annotation1747336;Name=-35 (rev);Parent=BBa_P1010
BBa_K145009	.	point_mutation	550	550	.	+	0	ID=annotation1747334;Name=T to C mutation;Parent=BBa_P1010
BBa_K145009	.	CDS	112	417	.	+	0	ID=annotation1747332;Name=ccdB (rev);Parent=BBa_P1010
BBa_K145009	.	sequence_feature	678	683	.	+	0	ID=annotation1747335;Name=-10 (rev);Parent=BBa_P1010
BBa_K145009	.	terminator	855	895	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K145009	.	stem_loop	862	881	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K145009	.	engineered_region	855	895	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K145009	.	polyA_site	882	895	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K145009	.	stop_codon	888	888	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K145009	.	terminator	767	846	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K145009	.	engineered_region	767	846	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K145009	.	stem_loop	778	821	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
>BBa_K145009
acctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatactagagaaagaggagaaatact
agagactggctgtgtataagggagcctgacatttatattccccagaacatcaggttaatggcgtttttgatgtcatttt
cgcggtggctgagatcagccacttcttccccgataacggagaccggcacactggccatatcggtggtcatcatgcgcca
gctttcatccccgatatgcaccaccgggtaaagttcacgggagactttatctgacagcagacgtgcactggccaggggg
atcaccatccgtcgcccgggcgtgtcaataatatcactctgtacatccacaaacagacgataacggctctctcttttat
aggtgtaaaccttaaactgcatttcaccagcccctgttctcgtcagcaaaagagccgttcatttcaataaaccgggcga
cctcagccatcccttcctgattttccgctttccagcgttcggcacgcagacgacgggcttcattctgcatggttgtgct
taccagaccggagatattgacatcatatatgccttgagcaactgatagctgtcgctgtcaactgtcactgtaatacgct
gcttcatagcatacctctttttgacatacttcgggtatacatatcagtatatattcttataccgcaaaaatcagcgcgc
aaatacgcatactgttatctggcttttagtaagccggatccacgcgttactagagccaggcatcaaataaaacgaaagg
ctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcacc
ttcgggtgggcctttctgcgtttata