LOCUS       BBa_K145010             1882 bp    DNA     linear       22-Nov-2024
DEFINITION  Killing switch
ACCESSION   BBa_K145010
VERSION     BBa_K145010.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23100
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     misc_feature    62..63
                     /label=prefix
     misc_feature    235..235
                     /label=T
     misc_feature    553..553
                     /label=A
     CDS             62..811
                     /label=luxR
     misc_feature    62..817
                     /label=BBa_C0062
     misc_feature    818..842
                     /label=Help:Barcodes
     CDS             62..817
                     /label=BBa_C0062
     stem_loop       862..905
                     /label=stem_loop
     misc_feature    851..930
                     /label=BBa_B0010
     terminator      851..930
                     /label=BBa_B0010
     stem_loop       946..965
                     /label=T7 TE
     misc_feature    972..972
                     /label=stop
     polyA_site      966..979
                     /label=polya
     misc_feature    939..979
                     /label=BBa_B0012
     terminator      939..979
                     /label=BBa_B0012
     misc_feature    44..979
                     /label=BBa_I0462
     misc_feature    1..979
                     /label=BBa_S03901
     misc_feature    1..979
                     /label=BBa_K145008
     misc_feature    988..1007
                     /label=LuxR/HSL
     promoter        1007..1012
                     /label=-35
     promoter        1029..1034
                     /label=-10
     misc_feature    1040..1040
                     /label=start
     misc_feature    988..1042
                     /label=BBa_R0062
     promoter        988..1042
                     /label=BBa_R0062
     misc_feature    1055..1058
                     /label=conserved
     RBS             1051..1062
                     /label=BBa_B0034
     CDS             1099..1404
                     /label=ccdB (rev)
     CDS             1406..1626
                     /label=ccdA- (rev)
     misc_feature    1537..1537
                     /label=T to C mutation
     misc_feature    1665..1670
                     /label=-10 (rev)
     misc_feature    1688..1693
                     /label=-35 (rev)
     misc_feature    1071..1745
                     /label=BBa_P1010
     stem_loop       1765..1808
                     /label=stem_loop
     misc_feature    1754..1833
                     /label=BBa_B0010
     terminator      1754..1833
                     /label=BBa_B0010
     stem_loop       1849..1868
                     /label=T7 TE
     misc_feature    1875..1875
                     /label=stop
     polyA_site      1869..1882
                     /label=polya
     misc_feature    1842..1882
                     /label=BBa_B0012
     terminator      1842..1882
                     /label=BBa_B0012
     misc_feature    988..1882
                     /label=BBa_K145009
ORIGIN
        1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagaaagagg agaaatacta
       61 gatgaaaaac ataaatgccg acgacacata cagaataatt aataaaatta aagcttgtag
      121 aagcaataat gatattaatc aatgcttatc tgatatgact aaaatggtac attgtgaata
      181 ttatttactc gcgatcattt atcctcattc tatggttaaa tctgatattt caatcctaga
      241 taattaccct aaaaaatgga ggcaatatta tgatgacgct aatttaataa aatatgatcc
      301 tatagtagat tattctaact ccaatcattc accaattaat tggaatatat ttgaaaacaa
      361 tgctgtaaat aaaaaatctc caaatgtaat taaagaagcg aaaacatcag gtcttatcac
      421 tgggtttagt ttccctattc atacggctaa caatggcttc ggaatgctta gttttgcaca
      481 ttcagaaaaa gacaactata tagatagttt atttttacat gcgtgtatga acataccatt
      541 aattgttcct tctctagttg ataattatcg aaaaataaat atagcaaata ataaatcaaa
      601 caacgattta accaaaagag aaaaagaatg tttagcgtgg gcatgcgaag gaaaaagctc
      661 ttgggatatt tcaaaaatat taggttgcag tgagcgtact gtcactttcc atttaaccaa
      721 tgcgcaaatg aaactcaata caacaaaccg ctgccaaagt atttctaaag caattttaac
      781 aggagcaatt gattgcccat actttaaaaa ttaataacac tgatagtgct agtgtagatc
      841 actactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt
      901 tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt
      961 gggcctttct gcgtttatat actagagacc tgtaggatcg tacaggttta cgcaagaaaa
     1021 tggtttgtta tagtcgaata aatactagag aaagaggaga aatactagag actggctgtg
     1081 tataagggag cctgacattt atattcccca gaacatcagg ttaatggcgt ttttgatgtc
     1141 attttcgcgg tggctgagat cagccacttc ttccccgata acggagaccg gcacactggc
     1201 catatcggtg gtcatcatgc gccagctttc atccccgata tgcaccaccg ggtaaagttc
     1261 acgggagact ttatctgaca gcagacgtgc actggccagg gggatcacca tccgtcgccc
     1321 gggcgtgtca ataatatcac tctgtacatc cacaaacaga cgataacggc tctctctttt
     1381 ataggtgtaa accttaaact gcatttcacc agcccctgtt ctcgtcagca aaagagccgt
     1441 tcatttcaat aaaccgggcg acctcagcca tcccttcctg attttccgct ttccagcgtt
     1501 cggcacgcag acgacgggct tcattctgca tggttgtgct taccagaccg gagatattga
     1561 catcatatat gccttgagca actgatagct gtcgctgtca actgtcactg taatacgctg
     1621 cttcatagca tacctctttt tgacatactt cgggtataca tatcagtata tattcttata
     1681 ccgcaaaaat cagcgcgcaa atacgcatac tgttatctgg cttttagtaa gccggatcca
     1741 cgcgttacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt
     1801 cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg
     1861 ggtgggcctt tctgcgttta ta
//