##gff-version 3
##sequence-region BBa_K145010 1 1882
BBa_K145010	.	engineered_region	1	979	.	+	0	ID=BBa_K145008;Name=BBa_K145008
BBa_K145010	.	engineered_region	1	979	.	+	0	ID=BBa_S03901;Name=BBa_S03901;Parent=BBa_K145008
BBa_K145010	.	engineered_region	44	979	.	+	0	ID=BBa_I0462;Name=BBa_I0462;Parent=BBa_S03901
BBa_K145010	.	terminator	851	930	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I0462
BBa_K145010	.	stem_loop	862	905	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K145010	.	engineered_region	851	930	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K145010	.	ribosome_entry_site	44	55	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I0462
BBa_K145010	.	conserved_region	48	51	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K145010	.	CDS	62	817	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_I0462
BBa_K145010	.	CDS	62	811	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K145010	.	engineered_region	62	817	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K145010	.	engineered_tag	818	842	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K145010	.	point_mutation	235	235	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K145010	.	engineered_region	62	63	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K145010	.	point_mutation	553	553	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_K145010	.	terminator	939	979	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I0462
BBa_K145010	.	engineered_region	939	979	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K145010	.	stop_codon	972	972	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K145010	.	stem_loop	946	965	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K145010	.	polyA_site	966	979	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K145010	.	promoter	1	35	.	+	0	ID=BBa_J23100;Name=BBa_J23100;Parent=BBa_S03901
BBa_K145010	.	engineered_region	988	1882	.	+	0	ID=BBa_K145009;Name=BBa_K145009
BBa_K145010	.	terminator	1842	1882	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K145009
BBa_K145010	.	engineered_region	1842	1882	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K145010	.	stop_codon	1875	1875	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K145010	.	stem_loop	1849	1868	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K145010	.	polyA_site	1869	1882	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K145010	.	terminator	1754	1833	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K145009
BBa_K145010	.	stem_loop	1765	1808	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K145010	.	engineered_region	1754	1833	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K145010	.	promoter	988	1042	.	+	0	ID=BBa_R0062;Name=BBa_R0062;Parent=BBa_K145009
BBa_K145010	.	non_covalent_binding_site	988	1007	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_K145010	.	promoter	1029	1034	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_K145010	.	engineered_region	988	1042	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_K145010	.	start_codon	1040	1040	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_K145010	.	promoter	1007	1012	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_K145010	.	ribosome_entry_site	1051	1062	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K145009
BBa_K145010	.	conserved_region	1055	1058	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K145010	.	engineered_region	1071	1745	.	+	0	ID=BBa_P1010;Name=BBa_P1010;Parent=BBa_K145009
BBa_K145010	.	sequence_feature	1688	1693	.	+	0	ID=annotation1747336;Name=-35 (rev);Parent=BBa_P1010
BBa_K145010	.	CDS	1406	1626	.	+	0	ID=annotation1747333;Name=ccdA- (rev);Parent=BBa_P1010
BBa_K145010	.	CDS	1099	1404	.	+	0	ID=annotation1747332;Name=ccdB (rev);Parent=BBa_P1010
BBa_K145010	.	point_mutation	1537	1537	.	+	0	ID=annotation1747334;Name=T to C mutation;Parent=BBa_P1010
BBa_K145010	.	sequence_feature	1665	1670	.	+	0	ID=annotation1747335;Name=-10 (rev);Parent=BBa_P1010
>BBa_K145010
ttgacggctagctcagtcctaggtacagtgctagctactagagaaagaggagaaatactagatgaaaaacataaatgcc
gacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatga
ctaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcct
agataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattct
aactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaag
aagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttt
tgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttct
ctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtt
tagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttcca
tttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaatt
gattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacg
aaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggc
tcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtttacgcaagaaaatggtttg
ttatagtcgaataaatactagagaaagaggagaaatactagagactggctgtgtataagggagcctgacatttatattc
cccagaacatcaggttaatggcgtttttgatgtcattttcgcggtggctgagatcagccacttcttccccgataacgga
gaccggcacactggccatatcggtggtcatcatgcgccagctttcatccccgatatgcaccaccgggtaaagttcacgg
gagactttatctgacagcagacgtgcactggccagggggatcaccatccgtcgcccgggcgtgtcaataatatcactct
gtacatccacaaacagacgataacggctctctcttttataggtgtaaaccttaaactgcatttcaccagcccctgttct
cgtcagcaaaagagccgttcatttcaataaaccgggcgacctcagccatcccttcctgattttccgctttccagcgttc
ggcacgcagacgacgggcttcattctgcatggttgtgcttaccagaccggagatattgacatcatatatgccttgagca
actgatagctgtcgctgtcaactgtcactgtaatacgctgcttcatagcatacctctttttgacatacttcgggtatac
atatcagtatatattcttataccgcaaaaatcagcgcgcaaatacgcatactgttatctggcttttagtaagccggatc
cacgcgttactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgttt
gtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata