LOCUS       BBa_K145014             2742 bp    DNA     linear       22-Dec-2024
DEFINITION  T7 RNA Polymerase with UmuD derived tag
ACCESSION   BBa_K145014
VERSION     BBa_K145014.1  
FEATURES             Location/Qualifiers
     misc_feature    1..3
                     /label=start
     misc_feature    2740..2742
                     /label=stop
     misc_feature    2737..2739
                     /label=stop
     misc_feature    1..87
                     /label=tag
     CDS             88..2736
                     /label=cds
ORIGIN
        1 atgttgttta tcaagcctgc ggatctccgc gaaattgtga cttttccgct atttagcgat
       61 cttgttcagt gtggctttcc ttcaccgatg aacacgatta acatcgctaa gaacgacttc
      121 tctgacatcg aactggctgc tatcccgttc aacactctgg ctgaccatta cggtgagcgt
      181 ttagctcgcg aacagttggc ccttgagcat gagtcttacg agatgggtga agcacgcttc
      241 cgcaagatgt ttgagcgtca acttaaagct ggtgaggttg cggataacgc tgccgccaag
      301 cctctcatca ctaccctact ccctaagatg attgcacgca tcaacgactg gtttgaggaa
      361 gtgaaagcta agcgcggcaa gcgcccgaca gccttccagt tcctgcaaga aatcaagccg
      421 gaagccgtag cgtacatcac cattaagacc actctggctt gcctaaccag tgctgacaat
      481 acaaccgttc aggctgtagc aagcgcaatc ggtcgggcca ttgaggacga ggctcgcttc
      541 ggtcgtatcc gtgaccttga agctaagcac ttcaagaaaa acgttgagga acaactcaac
      601 aagcgcgtag ggcacgtcta caagaaagca tttatgcaag ttgtcgaggc tgacatgctc
      661 tctaagggtc tactcggtgg cgaggcgtgg tcttcgtggc ataaggaaga ctctattcat
      721 gtaggagtac gctgcatcga gatgctcatt gagtcaaccg gaatggttag cttacaccgc
      781 caaaatgctg gcgtagtagg tcaagactct gagactatcg aactcgcacc tgaatacgct
      841 gaggctatcg caacccgtgc aggtgcgctg gctggcatct ctccgatgtt ccaaccttgc
      901 gtagttcctc ctaagccgtg gactggcatt actggtggtg gctattgggc taacggtcgt
      961 cgtcctctgg cgctggtgcg tactcacagt aagaaagcac tgatgcgcta cgaagacgtt
     1021 tacatgcctg aggtgtacaa agcgattaac attgcgcaaa acaccgcatg gaaaatcaac
     1081 aagaaagtcc tagcggtcgc caacgtaatc accaagtgga agcattgtcc ggtcgaggac
     1141 atccctgcga ttgagcgtga agaactcccg atgaaaccgg aagacatcga catgaatcct
     1201 gaggctctca ccgcgtggaa acgtgctgcc gctgctgtgt accgcaagga caaggctcgc
     1261 aagtctcgcc gtatcagcct tgagttcatg cttgagcaag ccaataagtt tgctaaccat
     1321 aaggccatct ggttccctta caacatggac tggcgcggtc gtgtttacgc tgtgtcaatg
     1381 ttcaacccgc aaggtaacga tatgaccaaa ggactgctta cgctggcgaa aggtaaacca
     1441 atcggtaagg aaggttacta ctggctgaaa atccacggtg caaactgtgc gggtgtcgat
     1501 aaggttccgt tccctgagcg catcaagttc attgaggaaa accacgagaa catcatggct
     1561 tgcgctaagt ctccactgga gaacacttgg tgggctgagc aagattctcc gttctgcttc
     1621 cttgcgttct gctttgagta cgctggggta cagcaccacg gcctgagcta taactgctcc
     1681 cttccgctgg cgtttgacgg gtcttgctct ggcatccagc acttctccgc gatgctccga
     1741 gatgaggtag gtggtcgcgc ggttaacttg cttcctagtg aaaccgttca ggacatctac
     1801 gggattgttg ctaagaaagt caacgagatt ctacaagcag acgcaatcaa tgggaccgat
     1861 aacgaagtag ttaccgtgac cgatgagaac actggtgaaa tctctgagaa agtcaagctg
     1921 ggcactaagg cactggctgg tcaatggctg gcttacggtg ttactcgcag tgtgactaag
     1981 cgttcagtca tgacgctggc ttacgggtcc aaagagttcg gcttccgtca acaagtgctg
     2041 gaagatacca ttcagccagc tattgattcc ggcaagggtc tgatgttcac tcagccgaat
     2101 caggctgctg gatacatggc taagctgatt tgggaatctg tgagcgtgac ggtggtagct
     2161 gcggttgaag caatgaactg gcttaagtct gctgctaagc tgctggctgc tgaggtcaaa
     2221 gataagaaga ctggagagat tcttcgcaag cgttgcgctg tgcattgggt aactcctgat
     2281 ggtttccctg tgtggcagga atacaagaag cctattcaga cgcgcttgaa cctgatgttc
     2341 ctcggtcagt tccgcttaca gcctaccatt aacaccaaca aagatagcga gattgatgca
     2401 cacaaacagg agtctggtat cgctcctaac tttgtacaca gccaagacgg tagccacctt
     2461 cgtaagactg tagtgtgggc acacgagaag tacggaatcg aatcttttgc actgattcac
     2521 gactccttcg gtaccattcc ggctgacgct gcgaacctgt tcaaagcagt gcgcgaaact
     2581 atggttgaca catatgagtc ttgtgatgta ctggctgatt tctacgacca gttcgctgac
     2641 cagttgcacg agtctcaatt ggacaaaatg ccagcacttc cggctaaagg taacttgaac
     2701 ctccgtgaca tcttagagtc ggacttcgcg ttcgcgtaat aa
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