##gff-version 3
##sequence-region BBa_K145100 1 1031
BBa_K145100	.	promoter	1	108	.	+	0	ID=BBa_R0084;Name=BBa_R0084
BBa_K145100	.	non_covalent_binding_site	51	68	.	+	0	ID=annotation301313;Name=F3 OmpR;Parent=BBa_R0084
BBa_K145100	.	non_covalent_binding_site	11	28	.	+	0	ID=annotation301308;Name=F1 OmpR;Parent=BBa_R0084
BBa_K145100	.	non_covalent_binding_site	31	48	.	+	0	ID=annotation301309;Name=F2 OmpR;Parent=BBa_R0084
BBa_K145100	.	promoter	88	93	.	+	0	ID=annotation301318;Name=-10;Parent=BBa_R0084
BBa_K145100	.	promoter	73	78	.	+	0	ID=annotation301317;Name=-35;Parent=BBa_R0084
BBa_K145100	.	terminator	903	982	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K145100	.	stem_loop	914	957	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K145100	.	engineered_region	903	982	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K145100	.	terminator	991	1031	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K145100	.	stem_loop	998	1017	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K145100	.	polyA_site	1018	1031	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K145100	.	stop_codon	1024	1024	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K145100	.	engineered_region	991	1031	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K145100	.	engineered_region	136	894	.	+	0	ID=BBa_K145015;Name=BBa_K145015
BBa_K145100	.	start_codon	136	138	.	+	0	ID=annotation1969616;Name=start;Parent=BBa_K145015
BBa_K145100	.	CDS	139	851	.	+	0	ID=annotation1969620;Name=cds;Parent=BBa_K145015
BBa_K145100	.	stop_codon	892	894	.	+	0	ID=annotation1969618;Name=stop;Parent=BBa_K145015
BBa_K145100	.	stop_codon	889	891	.	+	0	ID=annotation1969617;Name=stop;Parent=BBa_K145015
BBa_K145100	.	tag	852	888	.	+	0	ID=annotation1969619;Name=LVA tag;Parent=BBa_K145015
BBa_K145100	.	ribosome_entry_site	117	129	.	+	0	ID=BBa_B0032;Name=BBa_B0032
BBa_K145100	.	ribosome_entry_site	117	129	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K145100	.	ribosome_entry_site	123	126	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K145100	.	engineered_region	117	129	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
>BBa_K145100
cttaaattttacttttggttacatattttttctttttgaaaccaaatctttatctttgtagcactttcacggtagcgaa
acgttagtttgaatggaaagatgcctgcatactagagtcacacaggaaagtactagatgcgtaaaggagaagaactttt
cactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaa
ggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttg
tcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgc
catgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaag
tttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacaca
aattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaa
aattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccct
gtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatgg
tccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaaaggcctgctgcaaacgacga
aaactacgctttagtagcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctt
tcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtt
tata