##gff-version 3
##sequence-region BBa_K145101 1 2414
BBa_K145101	.	promoter	621	713	.	+	0	ID=BBa_I746364;Name=BBa_I746364
BBa_K145101	.	promoter	621	713	.	+	0	ID=annotation1943787;Name=Psid;Parent=BBa_I746364
BBa_K145101	.	CDS	741	1427	.	+	0	ID=BBa_C0053;Name=BBa_C0053
BBa_K145101	.	tag	1389	1421	.	+	0	ID=annotation1750;Name=LVA;Parent=BBa_C0053
BBa_K145101	.	engineered_tag	1428	1452	.	+	0	ID=annotation2213993;Name=Help:Barcodes;Parent=BBa_C0053
BBa_K145101	.	stop_codon	1422	1427	.	+	0	ID=annotation1751;Name=stop;Parent=BBa_C0053
BBa_K145101	.	CDS	741	1388	.	+	0	ID=annotation1747;Name=cII p22;Parent=BBa_C0053
BBa_K145101	.	engineered_region	741	1427	.	+	0	ID=annotation7036;Name=BBa_C0053;Parent=BBa_C0053
BBa_K145101	.	promoter	117	209	.	+	0	ID=BBa_I746364;Name=BBa_I746364
BBa_K145101	.	promoter	117	209	.	+	0	ID=annotation1943787;Name=Psid;Parent=BBa_I746364
BBa_K145101	.	promoter	1	108	.	+	0	ID=BBa_R0084;Name=BBa_R0084
BBa_K145101	.	promoter	88	93	.	+	0	ID=annotation301318;Name=-10;Parent=BBa_R0084
BBa_K145101	.	non_covalent_binding_site	11	28	.	+	0	ID=annotation301308;Name=F1 OmpR;Parent=BBa_R0084
BBa_K145101	.	non_covalent_binding_site	51	68	.	+	0	ID=annotation301313;Name=F3 OmpR;Parent=BBa_R0084
BBa_K145101	.	promoter	73	78	.	+	0	ID=annotation301317;Name=-35;Parent=BBa_R0084
BBa_K145101	.	non_covalent_binding_site	31	48	.	+	0	ID=annotation301309;Name=F2 OmpR;Parent=BBa_R0084
BBa_K145101	.	ribosome_entry_site	722	734	.	+	0	ID=BBa_B0032;Name=BBa_B0032
BBa_K145101	.	ribosome_entry_site	728	731	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K145101	.	engineered_region	722	734	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K145101	.	ribosome_entry_site	722	734	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K145101	.	ribosome_entry_site	218	230	.	+	0	ID=BBa_B0032;Name=BBa_B0032
BBa_K145101	.	ribosome_entry_site	224	227	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K145101	.	engineered_region	218	230	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K145101	.	ribosome_entry_site	218	230	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K145101	.	terminator	2286	2414	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K145101	.	terminator	2286	2365	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K145101	.	stem_loop	2297	2340	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K145101	.	engineered_region	2286	2365	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K145101	.	terminator	2374	2414	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K145101	.	engineered_region	2374	2414	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K145101	.	stop_codon	2407	2407	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K145101	.	stem_loop	2381	2400	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K145101	.	polyA_site	2401	2414	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K145101	.	terminator	1461	1589	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K145101	.	terminator	1461	1540	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K145101	.	stem_loop	1472	1515	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K145101	.	engineered_region	1461	1540	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K145101	.	terminator	1549	1589	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K145101	.	engineered_region	1549	1589	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K145101	.	stop_codon	1582	1582	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K145101	.	stem_loop	1556	1575	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K145101	.	polyA_site	1576	1589	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K145101	.	mature_transcript_region	1660	2277	.	+	0	ID=BBa_K145013;Name=BBa_K145013
BBa_K145101	.	promoter	1598	1651	.	+	0	ID=BBa_R0053;Name=BBa_R0053
BBa_K145101	.	promoter	1615	1620	.	+	0	ID=annotation2038;Name=-35;Parent=BBa_R0053
BBa_K145101	.	non_covalent_binding_site	1631	1648	.	+	0	ID=annotation2037;Name=OR1;Parent=BBa_R0053
BBa_K145101	.	non_covalent_binding_site	1598	1600	.	+	0	ID=annotation2035;Name=OR3;Parent=BBa_R0053
BBa_K145101	.	promoter	1627	1632	.	+	0	ID=annotation2042;Name=-10;Parent=BBa_R0053
BBa_K145101	.	engineered_region	1598	1651	.	+	0	ID=annotation7069;Name=BBa_R0053;Parent=BBa_R0053
BBa_K145101	.	non_covalent_binding_site	1608	1625	.	+	0	ID=annotation2036;Name=OR2;Parent=BBa_R0053
BBa_K145101	.	promoter	1605	1610	.	+	0	ID=annotation2041;Name=-35;Parent=BBa_R0053
BBa_K145101	.	engineered_region	239	475	.	+	0	ID=BBa_I746350;Name=BBa_I746350
BBa_K145101	.	start_codon	257	257	.	+	0	ID=annotation1943866;Name=P2 ogr;Parent=BBa_I746350
BBa_K145101	.	CDS	257	475	.	+	0	ID=annotation1943867;Name=P2 ogr;Parent=BBa_I746350
BBa_K145101	.	engineered_region	239	250	.	+	0	ID=annotation1943865;Name=B0034;Parent=BBa_I746350
BBa_K145101	.	terminator	484	612	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K145101	.	terminator	484	563	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K145101	.	stem_loop	495	538	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K145101	.	engineered_region	484	563	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K145101	.	terminator	572	612	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K145101	.	engineered_region	572	612	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K145101	.	stop_codon	605	605	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K145101	.	stem_loop	579	598	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K145101	.	polyA_site	599	612	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
>BBa_K145101
cttaaattttacttttggttacatattttttctttttgaaaccaaatctttatctttgtagcactttcacggtagcgaa
acgttagtttgaatggaaagatgcctgcatactagagtggcttgccggtctgaggatgagtctcctgtgtcagggctgg
cacatctgcaatgcgtcgtgttgttgtccggtgtacgtcacaattttcttatactagagtcacacaggaaagtactaga
gaaagaggagaaatactagatgtttcattgtcctttatgccagcatgccgcacatgcgcgtacaagtcgctatatcact
gacacgacaaaagagcgttatcatcagtgccagaacgtgaattgcagcgccacgttcatcacttatgagtcggtacagc
gatacatcgtgaagccgggagaagtccacgccgtaaggccgcacccgttgccatcagggcagcaaattatgtggatgta
atactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggt
gaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtggcttgccggt
ctgaggatgagtctcctgtgtcagggctggcacatctgcaatgcgtcgtgttgttgtccggtgtacgtcacaattttct
tatactagagtcacacaggaaagtactagatgaatacacaattgatgggtgagcgtattcgcgctcgaagaaaaaaact
caagattagacaagccgctcttggtaagatggtgggagtgtctaatgttgcaatatcgcaatgggagcgctcggagact
gagccaaatggggagaacctgttggcactttcgaaggctcttcagtgctcccctgactatttgctgaaaggagatttaa
gccagacaaacgttgcctatcatagtaggcatgagccaagaggatcataccctcttatcagttgggtaagcgcagggca
atggatggaagctgtagaaccttatcacaagcgcgcgatagagaactggcacgacaccactgtagattgttcagaagat
tcattttggcttgatgtccaaggtgactctatgacagcaccggcagggttaagcattccagaaggaatgataattctgg
ttgatcccgaagtcgaaccaagaaacggcaagctggttgttgcaaaattagaaggtgaaaacgaggccacattcaaaaa
attagttatggatgcaggccgaaagtttttaaaaccattaaacccacaatatccgatgatagaaatcaacggaaactgc
aaaatcattggcgtagttgttgacgcaaaactcgcaaatcttccagctgcaaacgacgaaaactacgctttagtagctt
aataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgg
gcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttct
gcgtttatatactagagaataaacttgactaaagattcctttagtagataatttaagtgttctttaatttctactagag
ttattaagctactaaagcgtagttttcgtcgtttgcagcatttaagactgcttttttaaactgttcattaataggcata
gacaatacaaccgatttagtatcacctaatacatgaatttctttgtctccaatacgatgacaaggaactttaatacgct
ttaaaaatcgctctattgctgttgatgttactgttacatattctgtaataccttgactaacagcgtgtttatatatagc
ttcaaatagtttcattgtaatttcactagcagagttatttatctttgagctatttttacctacagcaaaacgacttaat
tcgactatattaggatctttgggagcactctgttgaccaagcaattcaggaaaaacacttttcagcatataatcacctg
ttgtaggtaataaacgccagcatccacttacattttcagtatcatcacaagcataaatatattctgcatttgagttatc
atactcatctgattcaaggttattttctacaactaagtcccactcaagtctttgcttaaacacttgataacgaagactt
agaatacctttatactcctccgatggaattgccaaaaaatccgatttttttatcattatagtcattactagagccaggc
atcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctacta
gagtcacactggctcaccttcgggtgggcctttctgcgtttata