LOCUS       BBa_K145104             2263 bp    DNA     linear       10-May-2024
DEFINITION  T7 PoPS + RiboLock -> LacI -> inhibition of LuxI
ACCESSION   BBa_K145104
VERSION     BBa_K145104.1  
FEATURES             Location/Qualifiers
     promoter        1..46
                     /label=BBa_I712074
     misc_feature    55..97
                     /label=BBa_J23032
     misc_feature    1193..1231
                     /label=LVA
     CDS             104..1231
                     /label=lacI-LVA
     misc_feature    104..1231
                     /label=BBa_C0012
     misc_feature    1232..1256
                     /label=Help:Barcodes
     CDS             104..1231
                     /label=BBa_C0012
     stem_loop       1276..1319
                     /label=stem_loop
     misc_feature    1265..1344
                     /label=BBa_B0010
     terminator      1265..1344
                     /label=BBa_B0010
     stem_loop       1360..1379
                     /label=T7 TE
     misc_feature    1386..1386
                     /label=stop
     polyA_site      1380..1393
                     /label=polya
     misc_feature    1353..1393
                     /label=BBa_B0012
     terminator      1353..1393
                     /label=BBa_B0012
     terminator      1265..1393
                     /label=BBa_B0015
     misc_feature    1404..1420
                     /label=lac O1
     promoter        1421..1426
                     /label=-35
     misc_feature    1427..1443
                     /label=lac O1
     promoter        1444..1449
                     /label=-10
     misc_feature    1402..1455
                     /label=BBa_R0011
     promoter        1402..1455
                     /label=BBa_R0011
     RBS             1465..1477
                     /label=RBS-3\Weak
     RBS             1471..1474
                     /label=RBS
     misc_feature    1465..1477
                     /label=BBa_B0032
     RBS             1465..1477
                     /label=BBa_B0032
     misc_feature    2063..2094
                     /label=LVA
     CDS             1484..2062
                     /label=luxI
     misc_feature    1484..2101
                     /label=BBa_C0061
     misc_feature    2102..2126
                     /label=Help:Barcodes
     CDS             1484..2101
                     /label=BBa_C0061
     stem_loop       2146..2189
                     /label=stem_loop
     misc_feature    2135..2214
                     /label=BBa_B0010
     terminator      2135..2214
                     /label=BBa_B0010
     stem_loop       2230..2249
                     /label=T7 TE
     misc_feature    2256..2256
                     /label=stop
     polyA_site      2250..2263
                     /label=polya
     misc_feature    2223..2263
                     /label=BBa_B0012
     terminator      2223..2263
                     /label=BBa_B0012
     terminator      2135..2263
                     /label=BBa_B0015
ORIGIN
        1 taatacgact cactataggg aatacaagct acttgttctt tttgcatact agagaactag
       61 aatcacctct tgcttttggg taagacagaa gaggagatac tagatggtga atgtgaaacc
      121 agtaacgtta tacgatgtcg cagagtatgc cggtgtctct tatcagaccg tttcccgcgt
      181 ggtgaaccag gccagccacg tttctgcgaa aacgcgggaa aaagtggaag cggcgatggc
      241 ggagctgaat tacattccca accgcgtggc acaacaactg gcgggcaaac agtcgttgct
      301 gattggcgtt gccacctcca gtctggccct gcacgcgccg tcgcaaattg tcgcggcgat
      361 taaatctcgc gccgatcaac tgggtgccag cgtggtggtg tcgatggtag aacgaagcgg
      421 cgtcgaagcc tgtaaagcgg cggtgcacaa tcttctcgcg caacgcgtca gtgggctgat
      481 cattaactat ccgctggatg accaggatgc cattgctgtg gaagctgcct gcactaatgt
      541 tccggcgtta tttcttgatg tctctgacca gacacccatc aacagtatta ttttctccca
      601 tgaagacggt acgcgactgg gcgtggagca tctggtcgca ttgggtcacc agcaaatcgc
      661 gctgttagcg ggcccattaa gttctgtctc ggcgcgtctg cgtctggctg gctggcataa
      721 atatctcact cgcaatcaaa ttcagccgat agcggaacgg gaaggcgact ggagtgccat
      781 gtccggtttt caacaaacca tgcaaatgct gaatgagggc atcgttccca ctgcgatgct
      841 ggttgccaac gatcagatgg cgctgggcgc aatgcgcgcc attaccgagt ccgggctgcg
      901 cgttggtgcg gatatctcgg tagtgggata cgacgatacc gaagacagct catgttatat
      961 cccgccgtta accaccatca aacaggattt tcgcctgctg gggcaaacca gcgtggaccg
     1021 cttgctgcaa ctctctcagg gccaggcggt gaagggcaat cagctgttgc ccgtctcact
     1081 ggtgaaaaga aaaaccaccc tggcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     1141 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc aggctgcaaa
     1201 cgacgaaaac tacgctttag tagcttaata actctgatag tgctagtgta gatctctact
     1261 agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc
     1321 tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct
     1381 ttctgcgttt atatactaga gaattgtgag cggataacaa ttgacattgt gagcggataa
     1441 caagatactg agcacatact agagtcacac aggaaagtac tagatgacta taatgataaa
     1501 aaaatcggat tttttggcaa ttccatcgga ggagtataaa ggtattctaa gtcttcgtta
     1561 tcaagtgttt aagcaaagac ttgagtggga cttagttgta gaaaataacc ttgaatcaga
     1621 tgagtatgat aactcaaatg cagaatatat ttatgcttgt gatgatactg aaaatgtaag
     1681 tggatgctgg cgtttattac ctacaacagg tgattatatg ctgaaaagtg tttttcctga
     1741 attgcttggt caacagagtg ctcccaaaga tcctaatata gtcgaattaa gtcgttttgc
     1801 tgtaggtaaa aatagctcaa agataaataa ctctgctagt gaaattacaa tgaaactatt
     1861 tgaagctata tataaacacg ctgttagtca aggtattaca gaatatgtaa cagtaacatc
     1921 aacagcaata gagcgatttt taaagcgtat taaagttcct tgtcatcgta ttggagacaa
     1981 agaaattcat gtattaggtg atactaaatc ggttgtattg tctatgccta ttaatgaaca
     2041 gtttaaaaaa gcagtcttaa atgctgcaaa cgacgaaaac tacgctttag tagcttaata
     2101 actctgatag tgctagtgta gatctctact agagccaggc atcaaataaa acgaaaggct
     2161 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag
     2221 agtcacactg gctcaccttc gggtgggcct ttctgcgttt ata
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