BBa_K145105
type: ComponentDefinition
persistent identity: BBa K145105
display id: BBa_K145105
version: 1
was generated by: iGEM to SBOL conversion
description: cI under T7 and PR dual promotor
created: 2008-07-09T11:00:00Z
modified: 2015-05-08T01:10:28Z
mutable provenance: other parts
owned by: james
owned by: myers
mutable description: cI under T7 and PR dual promotor system. Should generate a pulse of cI.
discontinued: false
dominant: false
group u list: _257_
m user id: 0
owner id: 2970
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Model! Complete pulse generator by combining with BBa_K145106
star: false
creator: Jonas Demeulemeester
type: DnaRegion
role: partType/Other
role: SO:0000110
component: BBa_R0051
component: BBa_I712074
component: BBa_P0150
component: BBa_J23032
sequence annotation: BBa_I712074
sequence annotation: BBa_R0051
sequence annotation: BBa_J23032
sequence annotation: BBa_P0150
sequence: https://synbiohub.org/public/igem/BBa_K145105_sequence/1

BBa_R0051
type: Component
persistent identity: component2266633
display id: component2266633
version: 1
top level: BBa_K145105
definition: cI lam
access: public

BBa_I712074
type: Component
persistent identity: component2266631
display id: component2266631
version: 1
top level: BBa_K145105
definition: BBa_I712074
access: public

BBa_P0150
type: Component
persistent identity: component2266652
display id: component2266652
version: 1
top level: BBa_K145105
definition: BBa_P0150
access: public

BBa_J23032
type: Component
persistent identity: component2266637
display id: component2266637
version: 1
top level: BBa_K145105
definition: BBa_J23032
access: public

BBa_I712074
type: SequenceAnnotation
persistent identity: annotation2266631
display id: annotation2266631
version: 1
top level: BBa_K145105
location: https://synbiohub.org/public/igem/BBa_K145105/annotation2266631/range2266631/1
component: BBa_I712074

https://synbiohub.org/public/igem/BBa_K145105/annotation2266631/range2266631/1
type: Range
persistent identity: range2266631
display id: range2266631
version: 1
top level: BBa_K145105
direction: direction/reverse
start: 1
end: 46
orientation: inline

BBa_R0051
type: SequenceAnnotation
persistent identity: annotation2266633
display id: annotation2266633
version: 1
top level: BBa_K145105
location: https://synbiohub.org/public/igem/BBa_K145105/annotation2266633/range2266633/1
component: BBa_R0051

https://synbiohub.org/public/igem/BBa_K145105/annotation2266633/range2266633/1
type: Range
persistent identity: range2266633
display id: range2266633
version: 1
top level: BBa_K145105
direction: direction/forward
start: 55
end: 103
orientation: inline

BBa_J23032
type: SequenceAnnotation
persistent identity: annotation2266637
display id: annotation2266637
version: 1
top level: BBa_K145105
location: https://synbiohub.org/public/igem/BBa_K145105/annotation2266637/range2266637/1
component: BBa_J23032

https://synbiohub.org/public/igem/BBa_K145105/annotation2266637/range2266637/1
type: Range
persistent identity: range2266637
display id: range2266637
version: 1
top level: BBa_K145105
direction: direction/reverse
start: 112
end: 154
orientation: inline

BBa_P0150
type: SequenceAnnotation
persistent identity: annotation2266652
display id: annotation2266652
version: 1
top level: BBa_K145105
location: https://synbiohub.org/public/igem/BBa_K145105/annotation2266652/range2266652/1
component: BBa_P0150

https://synbiohub.org/public/igem/BBa_K145105/annotation2266652/range2266652/1
type: Range
persistent identity: range2266652
display id: range2266652
version: 1
top level: BBa_K145105
direction: direction/reverse
start: 163
end: 1088
orientation: inline

cI lam
type: ComponentDefinition
persistent identity: BBa R0051
display id: BBa_R0051
version: 1
was generated by: iGEM to SBOL conversion
description: promoter (lambda cI regulated)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: A synthetic oscillatory network of transcriptional regulators , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: The cI regulated promoter is based on the pR promtoer from bacteriohage lambda. The promoter has two two DNA binding sites for lambda cI repressor <bb_part>BBa_C0051</bb_part>. cI binding results in repression of transcription. The specific sequence used here is based on the cI repressible promoter used in the Elowitz repressilator (and references therein).
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: In order to address concerns about the promoter transcribing in the reverse direction, we have removed the -35 and -10 signals responsible for the promoter activity in the reverse direction. (More details needed here! DE, 2/24/03) Incompatible with host expressing cI repressor.
star: true
creator: Vinay S Mahajan, Brian Chow, Peter Carr, Voichita Marinescu and Alexander D. Wissner-Gross
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: BBa_R0051
sequence annotation: OR2
sequence annotation: -35
sequence annotation: OR1
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0051_sequence/1

BBa_R0051
type: SequenceAnnotation
persistent identity: annotation7067
display id: annotation7067
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_R0051/annotation7067/range7067/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_R0051/annotation7067/range7067/1
type: Range
persistent identity: range7067
display id: range7067
version: 1
top level: cI lam
direction: direction/forward
start: 1
end: 49
orientation: inline

OR2
type: SequenceAnnotation
persistent identity: annotation2025
display id: annotation2025
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_R0051/annotation2025/range2025/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0051/annotation2025/range2025/1
type: Range
persistent identity: range2025
display id: range2025
version: 1
top level: cI lam
direction: direction/forward
start: 1
end: 17
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2023
display id: annotation2023
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_R0051/annotation2023/range2023/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0051/annotation2023/range2023/1
type: Range
persistent identity: range2023
display id: range2023
version: 1
top level: cI lam
direction: direction/forward
start: 15
end: 20
orientation: inline

OR1
type: SequenceAnnotation
persistent identity: annotation2024
display id: annotation2024
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_R0051/annotation2024/range2024/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0051/annotation2024/range2024/1
type: Range
persistent identity: range2024
display id: range2024
version: 1
top level: cI lam
direction: direction/forward
start: 25
end: 41
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2022
display id: annotation2022
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_R0051/annotation2022/range2022/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0051/annotation2022/range2022/1
type: Range
persistent identity: range2022
display id: range2022
version: 1
top level: cI lam
direction: direction/forward
start: 38
end: 43
orientation: inline

BBa_I712074
type: ComponentDefinition
persistent identity: BBa I712074
display id: BBa_I712074
version: 1
was generated by: iGEM to SBOL conversion
description: T7 promoter (strong promoter from T7 bacteriophage)
created: 2007-10-21T11:00:00Z
modified: 2015-08-31T04:07:46Z
mutable provenance: T7 bacteriophage
owned by: james
owned by: myers
mutable description: T7 promoter is very specific promoter which is transcribed only by specific T7 RNA polymerase. Usually this promoter is used in expression systems where T7 promoter is cotransfected with T7 RNA polymerase. That ensures strong transcription of desired genes.
discontinued: false
dominant: false
experience: experience/Works
group u list: _130_
m user id: 0
owner id: 1856
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Rok Gaber
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I712074_sequence/1

BBa_J23032
type: ComponentDefinition
persistent identity: BBa J23032
display id: BBa_J23032
version: 1
was generated by: iGEM to SBOL conversion
description: [lock3d]
created: 2006-08-02T11:00:00Z
modified: 2015-08-31T04:08:39Z
mutable provenance: Extension of overlapping oligonucleotides
owned by: james
owned by: myers
mutable description: lock3d
discontinued: false
dominant: false
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: partType/RNA
role: SO:0000834
sequence: https://synbiohub.org/public/igem/BBa_J23032_sequence/1

BBa_P0150
type: ComponentDefinition
persistent identity: BBa P0150
display id: BBa_P0150
version: 1
was generated by: iGEM to SBOL conversion
description: PoPS -> cI (HK022) [S0164]
created: 2004-04-24T11:00:00Z
modified: 2015-05-08T01:14:10Z
owned by: james
owned by: myers
mutable description: Protein generator converting TIPS to the protein cI (HK022). Used as the input section for Quad Part Inverter Q01500
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
star: false
creator: Randy Rettberg
type: DnaRegion
role: partType/Generator
role: SO:0000804
component: BBa_B0012
component: BBa_B0010
component: BBa_C0050
component: BBa_B0031
sequence annotation: BBa_B0031
sequence annotation: BBa_C0050
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_P0150_sequence/1

BBa_B0012
type: Component
persistent identity: component944497
display id: component944497
version: 1
top level: BBa_P0150
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component944487
display id: component944487
version: 1
top level: BBa_P0150
definition: BBa_B0010
access: public

BBa_C0050
type: Component
persistent identity: component944479
display id: component944479
version: 1
top level: BBa_P0150
definition: cI HK022
access: public

BBa_B0031
type: Component
persistent identity: component944464
display id: component944464
version: 1
top level: BBa_P0150
definition: BBa_B0031
access: public

BBa_B0031
type: SequenceAnnotation
persistent identity: annotation944464
display id: annotation944464
version: 1
top level: BBa_P0150
location: https://synbiohub.org/public/igem/BBa_P0150/annotation944464/range944464/1
component: BBa_B0031

https://synbiohub.org/public/igem/BBa_P0150/annotation944464/range944464/1
type: Range
persistent identity: range944464
display id: range944464
version: 1
top level: BBa_P0150
direction: direction/forward
start: 1
end: 14
orientation: inline

BBa_C0050
type: SequenceAnnotation
persistent identity: annotation944479
display id: annotation944479
version: 1
top level: BBa_P0150
location: https://synbiohub.org/public/igem/BBa_P0150/annotation944479/range944479/1
component: BBa_C0050

https://synbiohub.org/public/igem/BBa_P0150/annotation944479/range944479/1
type: Range
persistent identity: range944479
display id: range944479
version: 1
top level: BBa_P0150
direction: direction/forward
start: 21
end: 764
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation944487
display id: annotation944487
version: 1
top level: BBa_P0150
location: https://synbiohub.org/public/igem/BBa_P0150/annotation944487/range944487/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_P0150/annotation944487/range944487/1
type: Range
persistent identity: range944487
display id: range944487
version: 1
top level: BBa_P0150
direction: direction/forward
start: 798
end: 877
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation944497
display id: annotation944497
version: 1
top level: BBa_P0150
location: https://synbiohub.org/public/igem/BBa_P0150/annotation944497/range944497/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_P0150/annotation944497/range944497/1
type: Range
persistent identity: range944497
display id: range944497
version: 1
top level: BBa_P0150
direction: direction/forward
start: 886
end: 926
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

BBa_B0031
type: ComponentDefinition
persistent identity: BBa B0031
display id: BBa_B0031
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.2 (weak) -- derivative of BBa_0030
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Medium RBS based on Ron Weiss thesis. Strength considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_44_48_46_1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-1" in figure 4-14 of thesis). No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Cho
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0031_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23316
display id: annotation23316
version: 1
top level: BBa_B0031
location: https://synbiohub.org/public/igem/BBa_B0031/annotation23316/range23316/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0031/annotation23316/range23316/1
type: Range
persistent identity: range23316
display id: range23316
version: 1
top level: BBa_B0031
direction: direction/forward
start: 7
end: 10
orientation: inline

cI HK022
type: ComponentDefinition
persistent identity: BBa C0050
display id: BBa_C0050
version: 1
was generated by: iGEM to SBOL conversion
description: cI repressor from phage HK022 (+LVA?)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: Bacteriophage HK022.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Coding region for the HK022 bacteriophage cI protein. cI binds to the HK022 pR regulator (BBa_R0050). It represses transcription of the protein encoded by the sequence 3' to the pR region. This coding sequence does not contain a RBS.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: References (unparsed) here: Carlson NG, Little JW. Highly cooperative DNA binding by the coliphage HK022 repressor. J Mol Biol. 1993 Apr 20;230(4):1108-30. PMID: 8487297. Mao C, Little JW. Mutations affecting cooperative DNA binding of phage HK022 CI repressor. J Mol Biol. 1998 May 29;279(1):31-48. PMID: 9636698. References (unparsed) here: Carlson NG, Little JW. Highly cooperative DNA binding by the coliphage HK022 repressor. J Mol Biol. 1993 Apr 20;230(4):1108-30. PMID: 8487297. Mao C, Little JW. Mutations affecting cooperative DNA binding of phage HK022 CI repressor. J Mol Biol. 1998 May 29;279(1):31-48. PMID: 9636698. Derived from <genbank>STHK022N</genbank>. Response from John Little (Arizona) regarding the start of HK022 cI From: <jlittle@email.arizona.edu> Date: Tue Jan 21, 2003 4:39:21 PM US/Eastern To: "Drew Endy" <endy@MIT.EDU> Subject: RE: hk022 cI start (naive question)? Hello Drew and Michael, I seriously doubt that the extra 27 aa are part of CI. It doesn't make sense in terms of the biology, for sure. In any case, the protein we characterized starts where Carlson and Little stated; as I recall, oR3 partially overlaps the start of cI. I have a vague memory of some possibly interesting biology, having to do with multicopy plasmids. I don't recall the findings themselves. Anyway, one possible explanation was that this extra N-terminal addition was made (e.g. from the pRE promoter, or from a message that arose from transcription around the entire plasmid), making a protein with altered functions. We never followed it up. Good luck John -- Original Message -- Date: Sun, 19 Jan 2003 15:26:26 -0500 Subject: hk022 cI start (naive question)? Cc: elowitm@rockefeller.edu To: jlittle@u.arizona.edu From: Drew Endy <endy@MIT.EDU> Hi John, I'm sitting here with Michael Elowitz and we're working through the sequence for HK022 cI. We noticed that the annotation from NC_002166 includes an "extra" 81 base pairs upstream of what we thought of as the actual start. It look…
star: true
creator: Reshma Shetty
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: BBa_C0050
sequence annotation: OR3 partial
sequence annotation: cI HK022
sequence annotation: SsrA
sequence annotation: 2
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0050_sequence/1

BBa_C0050
type: SequenceAnnotation
persistent identity: annotation7033
display id: annotation7033
version: 1
top level: cI HK022
location: https://synbiohub.org/public/igem/BBa_C0050/annotation7033/range7033/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_C0050/annotation7033/range7033/1
type: Range
persistent identity: range7033
display id: range7033
version: 1
top level: cI HK022
direction: direction/forward
start: 1
end: 744
orientation: inline

OR3 partial
type: SequenceAnnotation
persistent identity: annotation1737
display id: annotation1737
version: 1
top level: cI HK022
location: https://synbiohub.org/public/igem/BBa_C0050/annotation1737/range1737/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_C0050/annotation1737/range1737/1
type: Range
persistent identity: range1737
display id: range1737
version: 1
top level: cI HK022
direction: direction/forward
start: 1
end: 8
orientation: inline

cI HK022
type: SequenceAnnotation
persistent identity: annotation1735
display id: annotation1735
version: 1
top level: cI HK022
location: https://synbiohub.org/public/igem/BBa_C0050/annotation1735/range1735/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_C0050/annotation1735/range1735/1
type: Range
persistent identity: range1735
display id: range1735
version: 1
top level: cI HK022
direction: direction/forward
start: 1
end: 744
orientation: inline

SsrA
type: SequenceAnnotation
persistent identity: annotation1736
display id: annotation1736
version: 1
top level: cI HK022
location: https://synbiohub.org/public/igem/BBa_C0050/annotation1736/range1736/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_C0050/annotation1736/range1736/1
type: Range
persistent identity: range1736
display id: range1736
version: 1
top level: cI HK022
direction: direction/forward
start: 706
end: 738
orientation: inline

2
type: SequenceAnnotation
persistent identity: annotation1734
display id: annotation1734
version: 1
top level: cI HK022
location: https://synbiohub.org/public/igem/BBa_C0050/annotation1734/range1734/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_C0050/annotation1734/range1734/1
type: Range
persistent identity: range1734
display id: range1734
version: 1
top level: cI HK022
direction: direction/forward
start: 739
end: 744
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213990
display id: annotation2213990
version: 1
top level: cI HK022
location: https://synbiohub.org/public/igem/BBa_C0050/annotation2213990/range2213990/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_C0050/annotation2213990/range2213990/1
type: Range
persistent identity: range2213990
display id: range2213990
version: 1
top level: cI HK022
direction: direction/forward
start: 745
end: 769
orientation: inline

https://synbiohub.org/public/igem/BBa_K145105_sequence/1
type: Sequence
persistent identity: BBa K145105 sequence
display id: BBa_K145105_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taatacgactcactatagggaatacaagctacttgttctttttgcatactagagtaacaccgtgcgtgttgactattttacctctggcggtgataatggttgctactagagaactagaatcacctcttgcttttgggtaagacagaagaggagatactagagtcacacaggaaacctactagatggttcaacagaaagagcgtgaaactttctcgcagaggcttgcgctggcctgtgataaagcgggattacctttgcatggtaggcaggctgatttagctgtcaggcttaaggtcacaccaaaagccattagtaaatggttcaacggggagtcaataccaagaaaagacaagatggaatctctggcttcggtgctgggaactactgctgcatatctgcatggctatgctgatgatgacggtatcacggtaaatcatctatcaagatcaaatgattattatcgtgttgatgtattggatgttcaggcgagcgccgggccaggaaccatggtttccaatgaatttatagaaaagataagagcaattgaatatacgaccgagcaggcaagaattttatttaatggaaggccacaggaaagcgtaaaagtcatcacggttcgcggtgacagcatggagggaaccatcaatccgggagatgagatctttgttgatgtatccataacctgttttgatggcgatggcatttatgtgtttgtatacgggaaaacaatgcacgttaagcgcctgcaaatgcaaaagaacaggcttgccgtcatctctgacaatgccgcttatgatcgatggtacatagaagaaggtgaagaagagcaacttcacattctagccaaagtcctcattaggcagtcaatcgattacaagcgattcggagctgcaaacgacgaaaactacgctttagtagcttaataaccctgatagtgctagtgtagatccctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0051_sequence/1
type: Sequence
persistent identity: BBa R0051 sequence
display id: BBa_R0051_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taacaccgtgcgtgttgactattttacctctggcggtgataatggttgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I712074_sequence/1
type: Sequence
persistent identity: BBa I712074 sequence
display id: BBa_I712074_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taatacgactcactatagggaatacaagctacttgttctttttgca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23032_sequence/1
type: Sequence
persistent identity: BBa J23032 sequence
display id: BBa_J23032_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aactagaatcacctcttgcttttgggtaagacagaagaggaga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_P0150_sequence/1
type: Sequence
persistent identity: BBa P0150 sequence
display id: BBa_P0150_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaacctactagatggttcaacagaaagagcgtgaaactttctcgcagaggcttgcgctggcctgtgataaagcgggattacctttgcatggtaggcaggctgatttagctgtcaggcttaaggtcacaccaaaagccattagtaaatggttcaacggggagtcaataccaagaaaagacaagatggaatctctggcttcggtgctgggaactactgctgcatatctgcatggctatgctgatgatgacggtatcacggtaaatcatctatcaagatcaaatgattattatcgtgttgatgtattggatgttcaggcgagcgccgggccaggaaccatggtttccaatgaatttatagaaaagataagagcaattgaatatacgaccgagcaggcaagaattttatttaatggaaggccacaggaaagcgtaaaagtcatcacggttcgcggtgacagcatggagggaaccatcaatccgggagatgagatctttgttgatgtatccataacctgttttgatggcgatggcatttatgtgtttgtatacgggaaaacaatgcacgttaagcgcctgcaaatgcaaaagaacaggcttgccgtcatctctgacaatgccgcttatgatcgatggtacatagaagaaggtgaagaagagcaacttcacattctagccaaagtcctcattaggcagtcaatcgattacaagcgattcggagctgcaaacgacgaaaactacgctttagtagcttaataaccctgatagtgctagtgtagatccctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0031_sequence/1
type: Sequence
persistent identity: BBa B0031 sequence
display id: BBa_B0031_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaacc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0050_sequence/1
type: Sequence
persistent identity: BBa C0050 sequence
display id: BBa_C0050_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggttcaacagaaagagcgtgaaactttctcgcagaggcttgcgctggcctgtgataaagcgggattacctttgcatggtaggcaggctgatttagctgtcaggcttaaggtcacaccaaaagccattagtaaatggttcaacggggagtcaataccaagaaaagacaagatggaatctctggcttcggtgctgggaactactgctgcatatctgcatggctatgctgatgatgacggtatcacggtaaatcatctatcaagatcaaatgattattatcgtgttgatgtattggatgttcaggcgagcgccgggccaggaaccatggtttccaatgaatttatagaaaagataagagcaattgaatatacgaccgagcaggcaagaattttatttaatggaaggccacaggaaagcgtaaaagtcatcacggttcgcggtgacagcatggagggaaccatcaatccgggagatgagatctttgttgatgtatccataacctgttttgatggcgatggcatttatgtgtttgtatacgggaaaacaatgcacgttaagcgcctgcaaatgcaaaagaacaggcttgccgtcatctctgacaatgccgcttatgatcgatggtacatagaagaaggtgaagaagagcaacttcacattctagccaaagtcctcattaggcagtcaatcgattacaagcgattcggagctgcaaacgacgaaaactacgctttagtagcttaataaccctgatagtgctagtgtagatccc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z