##gff-version 3
##sequence-region BBa_K145107 1 2156
BBa_K145107	.	sequence_feature	1	1088	.	+	0	ID=BBa_K145105;Name=BBa_K145105
BBa_K145107	.	engineered_region	163	1088	.	+	0	ID=BBa_P0150;Name=BBa_P0150;Parent=BBa_K145105
BBa_K145107	.	terminator	960	1039	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_P0150
BBa_K145107	.	engineered_region	960	1039	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K145107	.	stem_loop	971	1014	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K145107	.	terminator	1048	1088	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_P0150
BBa_K145107	.	engineered_region	1048	1088	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K145107	.	stop_codon	1081	1081	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K145107	.	stem_loop	1055	1074	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K145107	.	polyA_site	1075	1088	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K145107	.	CDS	183	926	.	+	0	ID=BBa_C0050;Name=BBa_C0050;Parent=BBa_P0150
BBa_K145107	.	CDS	183	926	.	+	0	ID=annotation1735;Name=cI HK022;Parent=BBa_C0050
BBa_K145107	.	stop_codon	921	926	.	+	0	ID=annotation1734;Name=2;Parent=BBa_C0050
BBa_K145107	.	engineered_region	183	926	.	+	0	ID=annotation7033;Name=BBa_C0050;Parent=BBa_C0050
BBa_K145107	.	tag	888	920	.	+	0	ID=annotation1736;Name=SsrA;Parent=BBa_C0050
BBa_K145107	.	engineered_tag	927	951	.	+	0	ID=annotation2213990;Name=Help:Barcodes;Parent=BBa_C0050
BBa_K145107	.	non_covalent_binding_site	183	190	.	+	0	ID=annotation1737;Name=OR3 partial;Parent=BBa_C0050
BBa_K145107	.	ribosome_entry_site	163	176	.	+	0	ID=BBa_B0031;Name=BBa_B0031;Parent=BBa_P0150
BBa_K145107	.	conserved_region	169	172	.	+	0	ID=annotation23316;Name=conserved;Parent=BBa_B0031
BBa_K145107	.	promoter	1	46	.	+	0	ID=BBa_I712074;Name=BBa_I712074;Parent=BBa_K145105
BBa_K145107	.	promoter	55	103	.	+	0	ID=BBa_R0051;Name=BBa_R0051;Parent=BBa_K145105
BBa_K145107	.	promoter	92	97	.	+	0	ID=annotation2022;Name=-10;Parent=BBa_R0051
BBa_K145107	.	promoter	69	74	.	+	0	ID=annotation2023;Name=-35;Parent=BBa_R0051
BBa_K145107	.	engineered_region	55	103	.	+	0	ID=annotation7067;Name=BBa_R0051;Parent=BBa_R0051
BBa_K145107	.	non_covalent_binding_site	79	95	.	+	0	ID=annotation2024;Name=OR1;Parent=BBa_R0051
BBa_K145107	.	non_covalent_binding_site	55	71	.	+	0	ID=annotation2025;Name=OR2;Parent=BBa_R0051
BBa_K145107	.	mature_transcript_region	112	154	.	+	0	ID=BBa_J23032;Name=BBa_J23032;Parent=BBa_K145105
BBa_K145107	.	engineered_region	1097	2156	.	+	0	ID=BBa_K145106;Name=BBa_K145106
BBa_K145107	.	promoter	1097	1178	.	+	0	ID=BBa_I12007;Name=BBa_I12007;Parent=BBa_K145106
BBa_K145107	.	non_covalent_binding_site	1129	1145	.	+	0	ID=annotation937702;Name=OR2;Parent=BBa_I12007
BBa_K145107	.	sequence_alteration	1155	1161	.	+	0	ID=annotation937704;Name=mutate to obliterate OR3;Parent=BBa_I12007
BBa_K145107	.	promoter	1167	1172	.	+	0	ID=annotation937705;Name=-10;Parent=BBa_I12007
BBa_K145107	.	promoter	1144	1149	.	+	0	ID=annotation937703;Name=-35;Parent=BBa_I12007
BBa_K145107	.	non_covalent_binding_site	1105	1121	.	+	0	ID=annotation937701;Name=OR1;Parent=BBa_I12007
BBa_K145107	.	CDS	1206	1994	.	+	0	ID=BBa_C0060;Name=BBa_C0060;Parent=BBa_K145106
BBa_K145107	.	engineered_region	1206	1994	.	+	0	ID=annotation7037;Name=BBa_C0060;Parent=BBa_C0060
BBa_K145107	.	start_codon	1206	1208	.	+	0	ID=annotation1754;Name=start;Parent=BBa_C0060
BBa_K145107	.	engineered_tag	1995	2019	.	+	0	ID=annotation2213987;Name=Help:Barcodes;Parent=BBa_C0060
BBa_K145107	.	CDS	1206	1955	.	+	0	ID=annotation1757;Name=aiiA;Parent=BBa_C0060
BBa_K145107	.	tag	1956	1988	.	+	0	ID=annotation1756;Name=LVA;Parent=BBa_C0060
BBa_K145107	.	stop_codon	1989	1994	.	+	0	ID=annotation1755;Name=2;Parent=BBa_C0060
BBa_K145107	.	ribosome_entry_site	1187	1199	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_K145106
BBa_K145107	.	ribosome_entry_site	1193	1196	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K145107	.	ribosome_entry_site	1187	1199	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K145107	.	engineered_region	1187	1199	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K145107	.	terminator	2028	2156	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K145106
BBa_K145107	.	terminator	2028	2107	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K145107	.	engineered_region	2028	2107	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K145107	.	stem_loop	2039	2082	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K145107	.	terminator	2116	2156	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K145107	.	engineered_region	2116	2156	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K145107	.	stop_codon	2149	2149	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K145107	.	stem_loop	2123	2142	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K145107	.	polyA_site	2143	2156	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
>BBa_K145107
taatacgactcactatagggaatacaagctacttgttctttttgcatactagagtaacaccgtgcgtgttgactatttt
acctctggcggtgataatggttgctactagagaactagaatcacctcttgcttttgggtaagacagaagaggagatact
agagtcacacaggaaacctactagatggttcaacagaaagagcgtgaaactttctcgcagaggcttgcgctggcctgtg
ataaagcgggattacctttgcatggtaggcaggctgatttagctgtcaggcttaaggtcacaccaaaagccattagtaa
atggttcaacggggagtcaataccaagaaaagacaagatggaatctctggcttcggtgctgggaactactgctgcatat
ctgcatggctatgctgatgatgacggtatcacggtaaatcatctatcaagatcaaatgattattatcgtgttgatgtat
tggatgttcaggcgagcgccgggccaggaaccatggtttccaatgaatttatagaaaagataagagcaattgaatatac
gaccgagcaggcaagaattttatttaatggaaggccacaggaaagcgtaaaagtcatcacggttcgcggtgacagcatg
gagggaaccatcaatccgggagatgagatctttgttgatgtatccataacctgttttgatggcgatggcatttatgtgt
ttgtatacgggaaaacaatgcacgttaagcgcctgcaaatgcaaaagaacaggcttgccgtcatctctgacaatgccgc
ttatgatcgatggtacatagaagaaggtgaagaagagcaacttcacattctagccaaagtcctcattaggcagtcaatc
gattacaagcgattcggagctgcaaacgacgaaaactacgctttagtagcttaataaccctgatagtgctagtgtagat
ccctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcg
gtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagaggcaaccatta
tcaccgccagaggtaaaatagtcaacacgcacggtgttagatatttataaatagtggtgatagatttaacgttactaga
gtcacacaggaaagtactagatgacagtaaagaagctttatttcgtcccagcaggtcgttgtatgttggatcattcgtc
tgttaatagtacattaacaccaggagaattattagacttaccggtttggtgttatcttttggagactgaagaaggacct
attttagtagatacaggtatgccagaaagtgcagttaataatgaaggtctttttaacggtacatttgtcgaagggcagg
ttttaccgaaaatgactgaagaagatagaatcgtgaatattttaaaacgggttggttatgagccggaagaccttcttta
tattattagttctcacttgcattttgatcatgcaggaggaaatggcgcttttataaatacaccaatcattgtacagcgt
gctgaatatgaggcggcgcagcatagcgaagaatatttgaaagaatgtatattgccgaatttaaactacaaaatcattg
aaggtgattatgaagtcgtaccaggagttcaattattgcatacaccaggccatactccagggcatcaatcgctattaat
tgagacagaaaaatccggtcctgtattattaacgattgatgcatcgtatacgaaagagaattttgaaaatgaagtgcca
tttgcgggatttgattcagaattagctttatcttcaattaaacgtttaaaagaagtggtgatgaaagagaagccgattg
ttttctttggacatgatatagagcaggaaaggggatgtaaagtgttccctgaatatatagctgcaaacgacgaaaacta
cgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctc
agtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttc
gggtgggcctttctgcgtttata