##gff-version 3
##sequence-region BBa_K145110 1 1524
BBa_K145110	.	engineered_region	1	980	.	+	0	ID=BBa_K145108;Name=BBa_K145108
BBa_K145110	.	promoter	1	35	.	+	0	ID=BBa_J23100;Name=BBa_J23100;Parent=BBa_K145108
BBa_K145110	.	ribosome_entry_site	44	56	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_K145108
BBa_K145110	.	ribosome_entry_site	50	53	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K145110	.	ribosome_entry_site	44	56	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K145110	.	engineered_region	44	56	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K145110	.	CDS	63	818	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_K145108
BBa_K145110	.	point_mutation	554	554	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_K145110	.	engineered_region	63	818	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K145110	.	engineered_region	63	64	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K145110	.	CDS	63	812	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K145110	.	point_mutation	236	236	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K145110	.	engineered_tag	819	843	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K145110	.	terminator	852	980	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K145108
BBa_K145110	.	terminator	852	931	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K145110	.	stem_loop	863	906	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K145110	.	engineered_region	852	931	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K145110	.	terminator	940	980	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K145110	.	engineered_region	940	980	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K145110	.	stop_codon	973	973	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K145110	.	polyA_site	967	980	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K145110	.	stem_loop	947	966	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K145110	.	engineered_region	989	1524	.	+	0	ID=BBa_K145109;Name=BBa_K145109
BBa_K145110	.	terminator	1396	1475	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K145109
BBa_K145110	.	stem_loop	1407	1450	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K145110	.	engineered_region	1396	1475	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K145110	.	CDS	1082	1387	.	+	0	ID=BBa_K145151;Name=BBa_K145151;Parent=BBa_K145109
BBa_K145110	.	CDS	1082	1384	.	+	0	ID=annotation1970253;Name=cds;Parent=BBa_K145151
BBa_K145110	.	start_codon	1082	1084	.	+	0	ID=annotation1970251;Name=start;Parent=BBa_K145151
BBa_K145110	.	stop_codon	1385	1387	.	+	0	ID=annotation1970252;Name=stop;Parent=BBa_K145151
BBa_K145110	.	ribosome_entry_site	1063	1075	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_K145109
BBa_K145110	.	ribosome_entry_site	1069	1072	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K145110	.	ribosome_entry_site	1063	1075	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K145110	.	engineered_region	1063	1075	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K145110	.	terminator	1484	1524	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K145109
BBa_K145110	.	engineered_region	1484	1524	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K145110	.	stop_codon	1517	1517	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K145110	.	polyA_site	1511	1524	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K145110	.	stem_loop	1491	1510	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K145110	.	promoter	989	1054	.	+	0	ID=BBa_K145150;Name=BBa_K145150;Parent=BBa_K145109
BBa_K145110	.	non_covalent_binding_site	989	1008	.	+	0	ID=annotation1971582;Name=Lux-box;Parent=BBa_K145150
BBa_K145110	.	non_covalent_binding_site	1034	1051	.	+	0	ID=annotation1971586;Name=OR1;Parent=BBa_K145150
BBa_K145110	.	promoter	1008	1013	.	+	0	ID=annotation1971583;Name=-35;Parent=BBa_K145150
BBa_K145110	.	non_covalent_binding_site	1011	1028	.	+	0	ID=annotation1971584;Name=OR2;Parent=BBa_K145150
BBa_K145110	.	promoter	1030	1035	.	+	0	ID=annotation1971585;Name=-10;Parent=BBa_K145150
>BBa_K145110
ttgacggctagctcagtcctaggtacagtgctagctactagagtcacacaggaaagtactagatgaaaaacataaatgc
cgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatg
actaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcc
tagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattc
taactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaa
gaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagtt
ttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttc
tctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgt
ttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttcc
atttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaat
tgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaac
gaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactgg
ctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtttactaaagattcctttag
tttataatttaagtgttctttaatttctactagagtcacacaggaaagtactagatgcagtttaaggtttacacctata
aaagagagagccgttatcgtctgtttgtggatgtacagagtgatattattgacacgcccgggcgacggatggtgatccc
cctggccagtgcacgtctgctgtcagataaagtctcccgtgaactttacccggtggtgcatatcggggatgaaagctgg
cgcatgatgaccaccgatatggccagtgtgccggtctccgttatcggggaagaagtggctgatctcagccaccgcgaaa
atgacatcaaaaacgccattaacctgatgttctggggaatataatactagagccaggcatcaaataaaacgaaaggctc
agtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttc
gggtgggcctttctgcgtttata