##gff-version 3
##sequence-region BBa_K145111 1 2393
BBa_K145111	.	promoter	1577	1630	.	+	0	ID=BBa_R0053;Name=BBa_R0053
BBa_K145111	.	non_covalent_binding_site	1610	1627	.	+	0	ID=annotation2037;Name=OR1;Parent=BBa_R0053
BBa_K145111	.	promoter	1606	1611	.	+	0	ID=annotation2042;Name=-10;Parent=BBa_R0053
BBa_K145111	.	non_covalent_binding_site	1587	1604	.	+	0	ID=annotation2036;Name=OR2;Parent=BBa_R0053
BBa_K145111	.	promoter	1584	1589	.	+	0	ID=annotation2041;Name=-35;Parent=BBa_R0053
BBa_K145111	.	non_covalent_binding_site	1577	1579	.	+	0	ID=annotation2035;Name=OR3;Parent=BBa_R0053
BBa_K145111	.	engineered_region	1577	1630	.	+	0	ID=annotation7069;Name=BBa_R0053;Parent=BBa_R0053
BBa_K145111	.	promoter	1594	1599	.	+	0	ID=annotation2038;Name=-35;Parent=BBa_R0053
BBa_K145111	.	promoter	600	692	.	+	0	ID=BBa_I746364;Name=BBa_I746364
BBa_K145111	.	promoter	600	692	.	+	0	ID=annotation1943787;Name=Psid;Parent=BBa_I746364
BBa_K145111	.	promoter	1	108	.	+	0	ID=BBa_R0084;Name=BBa_R0084
BBa_K145111	.	non_covalent_binding_site	11	28	.	+	0	ID=annotation301308;Name=F1 OmpR;Parent=BBa_R0084
BBa_K145111	.	promoter	88	93	.	+	0	ID=annotation301318;Name=-10;Parent=BBa_R0084
BBa_K145111	.	non_covalent_binding_site	51	68	.	+	0	ID=annotation301313;Name=F3 OmpR;Parent=BBa_R0084
BBa_K145111	.	promoter	73	78	.	+	0	ID=annotation301317;Name=-35;Parent=BBa_R0084
BBa_K145111	.	non_covalent_binding_site	31	48	.	+	0	ID=annotation301309;Name=F2 OmpR;Parent=BBa_R0084
BBa_K145111	.	terminator	1440	1568	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K145111	.	terminator	1528	1568	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K145111	.	polyA_site	1555	1568	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K145111	.	engineered_region	1528	1568	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K145111	.	stem_loop	1535	1554	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K145111	.	stop_codon	1561	1561	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K145111	.	terminator	1440	1519	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K145111	.	stem_loop	1451	1494	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K145111	.	engineered_region	1440	1519	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K145111	.	promoter	117	209	.	+	0	ID=BBa_I746364;Name=BBa_I746364
BBa_K145111	.	promoter	117	209	.	+	0	ID=annotation1943787;Name=Psid;Parent=BBa_I746364
BBa_K145111	.	mature_transcript_region	1639	2256	.	+	0	ID=BBa_K145013;Name=BBa_K145013
BBa_K145111	.	engineered_region	218	454	.	+	0	ID=BBa_I746350;Name=BBa_I746350
BBa_K145111	.	engineered_region	218	229	.	+	0	ID=annotation1943865;Name=B0034;Parent=BBa_I746350
BBa_K145111	.	CDS	236	454	.	+	0	ID=annotation1943867;Name=P2 ogr;Parent=BBa_I746350
BBa_K145111	.	start_codon	236	236	.	+	0	ID=annotation1943866;Name=P2 ogr;Parent=BBa_I746350
BBa_K145111	.	CDS	720	1406	.	+	0	ID=BBa_C0053;Name=BBa_C0053
BBa_K145111	.	engineered_region	720	1406	.	+	0	ID=annotation7036;Name=BBa_C0053;Parent=BBa_C0053
BBa_K145111	.	engineered_tag	1407	1431	.	+	0	ID=annotation2213993;Name=Help:Barcodes;Parent=BBa_C0053
BBa_K145111	.	CDS	720	1367	.	+	0	ID=annotation1747;Name=cII p22;Parent=BBa_C0053
BBa_K145111	.	stop_codon	1401	1406	.	+	0	ID=annotation1751;Name=stop;Parent=BBa_C0053
BBa_K145111	.	tag	1368	1400	.	+	0	ID=annotation1750;Name=LVA;Parent=BBa_C0053
BBa_K145111	.	terminator	463	591	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K145111	.	terminator	551	591	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K145111	.	polyA_site	578	591	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K145111	.	engineered_region	551	591	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K145111	.	stem_loop	558	577	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K145111	.	stop_codon	584	584	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K145111	.	terminator	463	542	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K145111	.	stem_loop	474	517	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K145111	.	engineered_region	463	542	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K145111	.	ribosome_entry_site	701	713	.	+	0	ID=BBa_B0032;Name=BBa_B0032
BBa_K145111	.	engineered_region	701	713	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K145111	.	ribosome_entry_site	701	713	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K145111	.	ribosome_entry_site	707	710	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K145111	.	terminator	2265	2393	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K145111	.	terminator	2353	2393	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K145111	.	polyA_site	2380	2393	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K145111	.	engineered_region	2353	2393	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K145111	.	stem_loop	2360	2379	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K145111	.	stop_codon	2386	2386	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K145111	.	terminator	2265	2344	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K145111	.	stem_loop	2276	2319	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K145111	.	engineered_region	2265	2344	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
>BBa_K145111
cttaaattttacttttggttacatattttttctttttgaaaccaaatctttatctttgtagcactttcacggtagcgaa
acgttagtttgaatggaaagatgcctgcatactagagtggcttgccggtctgaggatgagtctcctgtgtcagggctgg
cacatctgcaatgcgtcgtgttgttgtccggtgtacgtcacaattttcttatactagagaaagaggagaaatactagat
gtttcattgtcctttatgccagcatgccgcacatgcgcgtacaagtcgctatatcactgacacgacaaaagagcgttat
catcagtgccagaacgtgaattgcagcgccacgttcatcacttatgagtcggtacagcgatacatcgtgaagccgggag
aagtccacgccgtaaggccgcacccgttgccatcagggcagcaaattatgtggatgtaatactagagccaggcatcaaa
taaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtca
cactggctcaccttcgggtgggcctttctgcgtttatatactagagtggcttgccggtctgaggatgagtctcctgtgt
cagggctggcacatctgcaatgcgtcgtgttgttgtccggtgtacgtcacaattttcttatactagagtcacacaggaa
agtactagatgaatacacaattgatgggtgagcgtattcgcgctcgaagaaaaaaactcaagattagacaagccgctct
tggtaagatggtgggagtgtctaatgttgcaatatcgcaatgggagcgctcggagactgagccaaatggggagaacctg
ttggcactttcgaaggctcttcagtgctcccctgactatttgctgaaaggagatttaagccagacaaacgttgcctatc
atagtaggcatgagccaagaggatcataccctcttatcagttgggtaagcgcagggcaatggatggaagctgtagaacc
ttatcacaagcgcgcgatagagaactggcacgacaccactgtagattgttcagaagattcattttggcttgatgtccaa
ggtgactctatgacagcaccggcagggttaagcattccagaaggaatgataattctggttgatcccgaagtcgaaccaa
gaaacggcaagctggttgttgcaaaattagaaggtgaaaacgaggccacattcaaaaaattagttatggatgcaggccg
aaagtttttaaaaccattaaacccacaatatccgatgatagaaatcaacggaaactgcaaaatcattggcgtagttgtt
gacgcaaaactcgcaaatcttccagctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagt
gtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgt
ttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagaata
aacttgactaaagattcctttagtagataatttaagtgttctttaatttctactagagttattaagctactaaagcgta
gttttcgtcgtttgcagcatttaagactgcttttttaaactgttcattaataggcatagacaatacaaccgatttagta
tcacctaatacatgaatttctttgtctccaatacgatgacaaggaactttaatacgctttaaaaatcgctctattgctg
ttgatgttactgttacatattctgtaataccttgactaacagcgtgtttatatatagcttcaaatagtttcattgtaat
ttcactagcagagttatttatctttgagctatttttacctacagcaaaacgacttaattcgactatattaggatctttg
ggagcactctgttgaccaagcaattcaggaaaaacacttttcagcatataatcacctgttgtaggtaataaacgccagc
atccacttacattttcagtatcatcacaagcataaatatattctgcatttgagttatcatactcatctgattcaaggtt
attttctacaactaagtcccactcaagtctttgcttaaacacttgataacgaagacttagaatacctttatactcctcc
gatggaattgccaaaaaatccgatttttttatcattatagtcattactagagccaggcatcaaataaaacgaaaggctc
agtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttc
gggtgggcctttctgcgtttata