BBa_K145111
type: ComponentDefinition
persistent identity: BBa K145111
display id: BBa_K145111
version: 1
was generated by: iGEM to SBOL conversion
description: OmpF PoPS -> deactivate constitutive antisense LuxI
created: 2008-07-09T11:00:00Z
modified: 2015-05-08T01:10:28Z
mutable provenance: other parts
owned by: james
owned by: myers
mutable description: Memory device in which P2ogr is under control of OmpF and Psid double promotor. P2ogr will also activate transcription of cII from a seperate Psid promotor. This cII represses transcription of antisense LuxI from a P22 promotor
discontinued: false
dominant: false
group u list: _257_
m user id: 0
owner id: 2970
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: validate dual promotor system!
star: false
creator: Jonas Demeulemeester
type: DnaRegion
role: partType/Other
role: SO:0000110
component: BBa_I746364
component: BBa_B0015
component: BBa_I746350
component: BBa_R0053
component: BBa_C0053
component: BBa_B0032
component: BBa_K145013
component: BBa_R0084
sequence annotation: BBa_R0084
sequence annotation: BBa_I746364
sequence annotation: BBa_I746350
sequence annotation: BBa_B0015
sequence annotation: BBa_B0032
sequence annotation: BBa_C0053
sequence annotation: BBa_R0053
sequence annotation: BBa_K145013
sequence: https://synbiohub.org/public/igem/BBa_K145111_sequence/1

BBa_I746364
type: Component
persistent identity: component2234194
display id: component2234194
version: 1
top level: BBa_K145111
definition: BBa_I746364
access: public

BBa_B0015
type: Component
persistent identity: component2234192
display id: component2234192
version: 1
top level: BBa_K145111
definition: BBa_B0015
access: public

BBa_I746350
type: Component
persistent identity: component2234185
display id: component2234185
version: 1
top level: BBa_K145111
definition: BBa_I746350
access: public

BBa_I746364
type: Component
persistent identity: component2234181
display id: component2234181
version: 1
top level: BBa_K145111
definition: BBa_I746364
access: public

BBa_R0053
type: Component
persistent identity: component2234211
display id: component2234211
version: 1
top level: BBa_K145111
definition: cII p22
access: public

BBa_B0015
type: Component
persistent identity: component2234209
display id: component2234209
version: 1
top level: BBa_K145111
definition: BBa_B0015
access: public

BBa_C0053
type: Component
persistent identity: component2234199
display id: component2234199
version: 1
top level: BBa_K145111
definition: c2 P22
access: public

BBa_B0032
type: Component
persistent identity: component2234196
display id: component2234196
version: 1
top level: BBa_K145111
definition: BBa_B0032
access: public

BBa_B0015
type: Component
persistent identity: component2234224
display id: component2234224
version: 1
top level: BBa_K145111
definition: BBa_B0015
access: public

BBa_K145013
type: Component
persistent identity: component2234217
display id: component2234217
version: 1
top level: BBa_K145111
definition: asLuxI
access: public

BBa_R0084
type: Component
persistent identity: component2234179
display id: component2234179
version: 1
top level: BBa_K145111
definition: OmpR
access: public

BBa_R0084
type: SequenceAnnotation
persistent identity: annotation2234179
display id: annotation2234179
version: 1
top level: BBa_K145111
location: https://synbiohub.org/public/igem/BBa_K145111/annotation2234179/range2234179/1
component: BBa_R0084

https://synbiohub.org/public/igem/BBa_K145111/annotation2234179/range2234179/1
type: Range
persistent identity: range2234179
display id: range2234179
version: 1
top level: BBa_K145111
direction: direction/reverse
start: 1
end: 108
orientation: inline

BBa_I746364
type: SequenceAnnotation
persistent identity: annotation2234181
display id: annotation2234181
version: 1
top level: BBa_K145111
location: https://synbiohub.org/public/igem/BBa_K145111/annotation2234181/range2234181/1
component: BBa_I746364

https://synbiohub.org/public/igem/BBa_K145111/annotation2234181/range2234181/1
type: Range
persistent identity: range2234181
display id: range2234181
version: 1
top level: BBa_K145111
direction: direction/reverse
start: 117
end: 209
orientation: inline

BBa_I746350
type: SequenceAnnotation
persistent identity: annotation2234185
display id: annotation2234185
version: 1
top level: BBa_K145111
location: https://synbiohub.org/public/igem/BBa_K145111/annotation2234185/range2234185/1
component: BBa_I746350

https://synbiohub.org/public/igem/BBa_K145111/annotation2234185/range2234185/1
type: Range
persistent identity: range2234185
display id: range2234185
version: 1
top level: BBa_K145111
direction: direction/reverse
start: 218
end: 454
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2234192
display id: annotation2234192
version: 1
top level: BBa_K145111
location: https://synbiohub.org/public/igem/BBa_K145111/annotation2234192/range2234192/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K145111/annotation2234192/range2234192/1
type: Range
persistent identity: range2234192
display id: range2234192
version: 1
top level: BBa_K145111
direction: direction/reverse
start: 463
end: 591
orientation: inline

BBa_I746364
type: SequenceAnnotation
persistent identity: annotation2234194
display id: annotation2234194
version: 1
top level: BBa_K145111
location: https://synbiohub.org/public/igem/BBa_K145111/annotation2234194/range2234194/1
component: BBa_I746364

https://synbiohub.org/public/igem/BBa_K145111/annotation2234194/range2234194/1
type: Range
persistent identity: range2234194
display id: range2234194
version: 1
top level: BBa_K145111
direction: direction/reverse
start: 600
end: 692
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2234196
display id: annotation2234196
version: 1
top level: BBa_K145111
location: https://synbiohub.org/public/igem/BBa_K145111/annotation2234196/range2234196/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K145111/annotation2234196/range2234196/1
type: Range
persistent identity: range2234196
display id: range2234196
version: 1
top level: BBa_K145111
direction: direction/forward
start: 701
end: 713
orientation: inline

BBa_C0053
type: SequenceAnnotation
persistent identity: annotation2234199
display id: annotation2234199
version: 1
top level: BBa_K145111
location: https://synbiohub.org/public/igem/BBa_K145111/annotation2234199/range2234199/1
component: BBa_C0053

https://synbiohub.org/public/igem/BBa_K145111/annotation2234199/range2234199/1
type: Range
persistent identity: range2234199
display id: range2234199
version: 1
top level: BBa_K145111
direction: direction/forward
start: 720
end: 1406
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2234209
display id: annotation2234209
version: 1
top level: BBa_K145111
location: https://synbiohub.org/public/igem/BBa_K145111/annotation2234209/range2234209/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K145111/annotation2234209/range2234209/1
type: Range
persistent identity: range2234209
display id: range2234209
version: 1
top level: BBa_K145111
direction: direction/reverse
start: 1440
end: 1568
orientation: inline

BBa_R0053
type: SequenceAnnotation
persistent identity: annotation2234211
display id: annotation2234211
version: 1
top level: BBa_K145111
location: https://synbiohub.org/public/igem/BBa_K145111/annotation2234211/range2234211/1
component: BBa_R0053

https://synbiohub.org/public/igem/BBa_K145111/annotation2234211/range2234211/1
type: Range
persistent identity: range2234211
display id: range2234211
version: 1
top level: BBa_K145111
direction: direction/forward
start: 1577
end: 1630
orientation: inline

BBa_K145013
type: SequenceAnnotation
persistent identity: annotation2234217
display id: annotation2234217
version: 1
top level: BBa_K145111
location: https://synbiohub.org/public/igem/BBa_K145111/annotation2234217/range2234217/1
component: BBa_K145013

https://synbiohub.org/public/igem/BBa_K145111/annotation2234217/range2234217/1
type: Range
persistent identity: range2234217
display id: range2234217
version: 1
top level: BBa_K145111
direction: direction/reverse
start: 1639
end: 2256
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2234224
display id: annotation2234224
version: 1
top level: BBa_K145111
location: https://synbiohub.org/public/igem/BBa_K145111/annotation2234224/range2234224/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K145111/annotation2234224/range2234224/1
type: Range
persistent identity: range2234224
display id: range2234224
version: 1
top level: BBa_K145111
direction: direction/reverse
start: 2265
end: 2393
orientation: inline

BBa_B0032
type: ComponentDefinition
persistent identity: BBa B0032
display id: BBa_B0032
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.3 (medium) -- derivative of BBa_0030
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_44_48_46_1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-2" in figure 4-14 of thesis). Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: RBS-3\Weak
sequence annotation: BBa_B0032
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0032_sequence/1

RBS-3\Weak
type: SequenceAnnotation
persistent identity: annotation1709
display id: annotation1709
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
type: Range
persistent identity: range1709
display id: range1709
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation7027
display id: annotation7027
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
type: Range
persistent identity: range7027
display id: range7027
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1710
display id: annotation1710
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
type: Range
persistent identity: range1710
display id: range1710
version: 1
top level: BBa_B0032
direction: direction/forward
start: 7
end: 10
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

OmpR
type: ComponentDefinition
persistent identity: BBa R0084
display id: BBa_R0084
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter (OmpR, positive)
created: 2004-01-27T12:00:00Z
modified: 2015-05-08T01:14:15Z
mutable provenance: NC_000913 E. coli K12
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Positively regulated, OmpR-controlled promoter. This promoter is taken from the upstream region of ompF. Phosphorylated OmpR binds to the three operator sites and activates transcription.
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: The promoter includes three OmpR binding sites: F1, F2, and F3. The promoter starts at -107 and goes up to the transcriptional start site at +1. A distant fourth binding site, F4, has been deleted. It is involved in negative regulation of ompF (Head, Tardy and Kenney, 1998), so its deletion is intended to make the promoter solely activating. The efficacy of this promoter versus the ompC upstream region (BBa_R0082) or the truncated ompC upstream region (BBa_R0083) is currently unknown.
star: true
creator: Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004)
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: F1 OmpR
sequence annotation: F2 OmpR
sequence annotation: F3 OmpR
sequence annotation: -35
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0084_sequence/1

F1 OmpR
type: SequenceAnnotation
persistent identity: annotation301308
display id: annotation301308
version: 1
top level: OmpR
location: https://synbiohub.org/public/igem/BBa_R0084/annotation301308/range301308/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0084/annotation301308/range301308/1
type: Range
persistent identity: range301308
display id: range301308
version: 1
top level: OmpR
direction: direction/forward
start: 11
end: 28
orientation: inline

F2 OmpR
type: SequenceAnnotation
persistent identity: annotation301309
display id: annotation301309
version: 1
top level: OmpR
location: https://synbiohub.org/public/igem/BBa_R0084/annotation301309/range301309/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0084/annotation301309/range301309/1
type: Range
persistent identity: range301309
display id: range301309
version: 1
top level: OmpR
direction: direction/forward
start: 31
end: 48
orientation: inline

F3 OmpR
type: SequenceAnnotation
persistent identity: annotation301313
display id: annotation301313
version: 1
top level: OmpR
location: https://synbiohub.org/public/igem/BBa_R0084/annotation301313/range301313/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0084/annotation301313/range301313/1
type: Range
persistent identity: range301313
display id: range301313
version: 1
top level: OmpR
direction: direction/forward
start: 51
end: 68
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation301317
display id: annotation301317
version: 1
top level: OmpR
location: https://synbiohub.org/public/igem/BBa_R0084/annotation301317/range301317/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0084/annotation301317/range301317/1
type: Range
persistent identity: range301317
display id: range301317
version: 1
top level: OmpR
direction: direction/forward
start: 73
end: 78
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation301318
display id: annotation301318
version: 1
top level: OmpR
location: https://synbiohub.org/public/igem/BBa_R0084/annotation301318/range301318/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0084/annotation301318/range301318/1
type: Range
persistent identity: range301318
display id: range301318
version: 1
top level: OmpR
direction: direction/forward
start: 88
end: 93
orientation: inline

asLuxI
type: ComponentDefinition
persistent identity: BBa K145013
display id: BBa_K145013
version: 1
was generated by: iGEM to SBOL conversion
description: antisense LuxI
created: 2008-07-09T11:00:00Z
modified: 2015-05-08T01:10:28Z
mutable provenance: BBa_C0061
owned by: james
owned by: myers
mutable description: antisense LuxI
discontinued: false
dominant: false
experience: experience/None
group u list: _257_
m user id: 0
owner id: 2970
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: just antisense
star: true
creator: Jonas Demeulemeester
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence: https://synbiohub.org/public/igem/BBa_K145013_sequence/1

c2 P22
type: ComponentDefinition
persistent identity: BBa C0053
display id: BBa_C0053
version: 1
was generated by: iGEM to SBOL conversion
description: c2 repressor from Salmonella phage P22 (+LVA)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: Bacteriophage P22
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: The P22 c2 repressor protein coding sequence is a 720 base-pair sequence with the standard RBS-compatible BioBrick prefix and the standard BioBrick suffix sections on its ends. It binds to the P22 c2 regulatory sequence, BBa_R0053. The sequence contains a LVA tag for faster degredation.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: References (unparsed) here: Vander Byl,C. and Kropinski,A.M. Sequence of the genome of Salmonella bacteriophage P22 J. Bacteriol. 182 (22), 6472-6481 (2000) References (unparsed) here: Vander Byl,C. and Kropinski,A.M. Sequence of the genome of Salmonella bacteriophage P22 J. Bacteriol. 182 (22), 6472-6481 (2000)
star: true
creator: Maia Mahoney
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: cII p22
sequence annotation: BBa_C0053
sequence annotation: LVA
sequence annotation: stop
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0053_sequence/1

cII p22
type: SequenceAnnotation
persistent identity: annotation1747
display id: annotation1747
version: 1
top level: c2 P22
location: https://synbiohub.org/public/igem/BBa_C0053/annotation1747/range1747/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_C0053/annotation1747/range1747/1
type: Range
persistent identity: range1747
display id: range1747
version: 1
top level: c2 P22
direction: direction/forward
start: 1
end: 648
orientation: inline

BBa_C0053
type: SequenceAnnotation
persistent identity: annotation7036
display id: annotation7036
version: 1
top level: c2 P22
location: https://synbiohub.org/public/igem/BBa_C0053/annotation7036/range7036/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_C0053/annotation7036/range7036/1
type: Range
persistent identity: range7036
display id: range7036
version: 1
top level: c2 P22
direction: direction/forward
start: 1
end: 687
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation1750
display id: annotation1750
version: 1
top level: c2 P22
location: https://synbiohub.org/public/igem/BBa_C0053/annotation1750/range1750/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_C0053/annotation1750/range1750/1
type: Range
persistent identity: range1750
display id: range1750
version: 1
top level: c2 P22
direction: direction/forward
start: 649
end: 681
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1751
display id: annotation1751
version: 1
top level: c2 P22
location: https://synbiohub.org/public/igem/BBa_C0053/annotation1751/range1751/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_C0053/annotation1751/range1751/1
type: Range
persistent identity: range1751
display id: range1751
version: 1
top level: c2 P22
direction: direction/forward
start: 682
end: 687
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213993
display id: annotation2213993
version: 1
top level: c2 P22
location: https://synbiohub.org/public/igem/BBa_C0053/annotation2213993/range2213993/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_C0053/annotation2213993/range2213993/1
type: Range
persistent identity: range2213993
display id: range2213993
version: 1
top level: c2 P22
direction: direction/forward
start: 688
end: 712
orientation: inline

BBa_I746350
type: ComponentDefinition
persistent identity: BBa I746350
display id: BBa_I746350
version: 1
was generated by: iGEM to SBOL conversion
description: ogr activator from P2 phage
created: 2007-09-10T11:00:00Z
modified: 2015-08-31T04:08:04Z
mutable provenance: plasmid DNA supplied by Prof. Richard Calendar, University of California.
owned by: james
owned by: myers
mutable description: The ogr activator taken from P2 phage acts on a class of inducible promoters (parts I746360 to I746365), inducing their activity to varying degrees. The part sequence does already contain a ribosome binding site (B0034)!
discontinued: false
dominant: false
experience: experience/None
group u list: _116_
m user id: 0
owner id: 2122
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: The part does contain a RBS (B0034) already.
star: true
creator: Stefan Milde
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: B0034
sequence annotation: P2 ogr
sequence: https://synbiohub.org/public/igem/BBa_I746350_sequence/1

B0034
type: SequenceAnnotation
persistent identity: annotation1943865
display id: annotation1943865
version: 1
top level: BBa_I746350
location: https://synbiohub.org/public/igem/BBa_I746350/annotation1943865/range1943865/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_I746350/annotation1943865/range1943865/1
type: Range
persistent identity: range1943865
display id: range1943865
version: 1
top level: BBa_I746350
direction: direction/forward
start: 1
end: 12
orientation: inline

P2 ogr
type: SequenceAnnotation
persistent identity: annotation1943867
display id: annotation1943867
version: 1
top level: BBa_I746350
location: https://synbiohub.org/public/igem/BBa_I746350/annotation1943867/range1943867/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_I746350/annotation1943867/range1943867/1
type: Range
persistent identity: range1943867
display id: range1943867
version: 1
top level: BBa_I746350
direction: direction/forward
start: 19
end: 237
orientation: inline

P2 ogr
type: SequenceAnnotation
persistent identity: annotation1943866
display id: annotation1943866
version: 1
top level: BBa_I746350
location: https://synbiohub.org/public/igem/BBa_I746350/annotation1943866/range1943866/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_I746350/annotation1943866/range1943866/1
type: Range
persistent identity: range1943866
display id: range1943866
version: 1
top level: BBa_I746350
direction: direction/forward
start: 19
end: 19
orientation: inline

BBa_I746364
type: ComponentDefinition
persistent identity: BBa I746364
display id: BBa_I746364
version: 1
was generated by: iGEM to SBOL conversion
description: Psid promoter from P4 phage
created: 2007-09-10T11:00:00Z
modified: 2015-08-31T04:08:04Z
mutable provenance: P4 phage genome
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: This is the Psid promoter taken from the P4 phage genome. It is an inducible promoter that is activated by a class of activators, including P2 ogr (I746350), PSP3 pag (I746351) and phiR73 delta (I746352). These different activators should cause different levels of activity of the Psid promoter.
discontinued: false
dominant: false
group u list: _116_
m user id: 0
owner id: 2122
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: no special considerations
star: true
creator: Stefan Milde
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: Psid
sequence: https://synbiohub.org/public/igem/BBa_I746364_sequence/1

Psid
type: SequenceAnnotation
persistent identity: annotation1943787
display id: annotation1943787
version: 1
top level: BBa_I746364
location: https://synbiohub.org/public/igem/BBa_I746364/annotation1943787/range1943787/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_I746364/annotation1943787/range1943787/1
type: Range
persistent identity: range1943787
display id: range1943787
version: 1
top level: BBa_I746364
direction: direction/forward
start: 1
end: 93
orientation: inline

cII p22
type: ComponentDefinition
persistent identity: BBa R0053
display id: BBa_R0053
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter (p22 cII regulated)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: Bacteriophage p22.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: The p22 cII regulatory region sequence is a 97 base-pair sequence with the standard BioBrick prefix and suffix sections on its ends. p22 cII repressor protein, BBa_C0053, binds to it. This segment contains O-R1, O-R2, a fragment of O-R3, the -35 of P-RM, and P-R (-10 and -35 from Tom Knight)
discontinued: false
dominant: false
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Maia Mahoney
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0053
sequence annotation: OR3
sequence annotation: -35
sequence annotation: OR2
sequence annotation: -10
sequence annotation: OR1
sequence: https://synbiohub.org/public/igem/BBa_R0053_sequence/1

BBa_R0053
type: SequenceAnnotation
persistent identity: annotation7069
display id: annotation7069
version: 1
top level: cII p22
location: https://synbiohub.org/public/igem/BBa_R0053/annotation7069/range7069/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0053/annotation7069/range7069/1
type: Range
persistent identity: range7069
display id: range7069
version: 1
top level: cII p22
direction: direction/forward
start: 1
end: 54
orientation: inline

OR3
type: SequenceAnnotation
persistent identity: annotation2035
display id: annotation2035
version: 1
top level: cII p22
location: https://synbiohub.org/public/igem/BBa_R0053/annotation2035/range2035/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0053/annotation2035/range2035/1
type: Range
persistent identity: range2035
display id: range2035
version: 1
top level: cII p22
direction: direction/forward
start: 1
end: 3
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2041
display id: annotation2041
version: 1
top level: cII p22
location: https://synbiohub.org/public/igem/BBa_R0053/annotation2041/range2041/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0053/annotation2041/range2041/1
type: Range
persistent identity: range2041
display id: range2041
version: 1
top level: cII p22
direction: direction/forward
start: 8
end: 13
orientation: inline

OR2
type: SequenceAnnotation
persistent identity: annotation2036
display id: annotation2036
version: 1
top level: cII p22
location: https://synbiohub.org/public/igem/BBa_R0053/annotation2036/range2036/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0053/annotation2036/range2036/1
type: Range
persistent identity: range2036
display id: range2036
version: 1
top level: cII p22
direction: direction/forward
start: 11
end: 28
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2038
display id: annotation2038
version: 1
top level: cII p22
location: https://synbiohub.org/public/igem/BBa_R0053/annotation2038/range2038/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0053/annotation2038/range2038/1
type: Range
persistent identity: range2038
display id: range2038
version: 1
top level: cII p22
direction: direction/forward
start: 18
end: 23
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2042
display id: annotation2042
version: 1
top level: cII p22
location: https://synbiohub.org/public/igem/BBa_R0053/annotation2042/range2042/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0053/annotation2042/range2042/1
type: Range
persistent identity: range2042
display id: range2042
version: 1
top level: cII p22
direction: direction/forward
start: 30
end: 35
orientation: inline

OR1
type: SequenceAnnotation
persistent identity: annotation2037
display id: annotation2037
version: 1
top level: cII p22
location: https://synbiohub.org/public/igem/BBa_R0053/annotation2037/range2037/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0053/annotation2037/range2037/1
type: Range
persistent identity: range2037
display id: range2037
version: 1
top level: cII p22
direction: direction/forward
start: 34
end: 51
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

https://synbiohub.org/public/igem/BBa_K145111_sequence/1
type: Sequence
persistent identity: BBa K145111 sequence
display id: BBa_K145111_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cttaaattttacttttggttacatattttttctttttgaaaccaaatctttatctttgtagcactttcacggtagcgaaacgttagtttgaatggaaagatgcctgcatactagagtggcttgccggtctgaggatgagtctcctgtgtcagggctggcacatctgcaatgcgtcgtgttgttgtccggtgtacgtcacaattttcttatactagagaaagaggagaaatactagatgtttcattgtcctttatgccagcatgccgcacatgcgcgtacaagtcgctatatcactgacacgacaaaagagcgttatcatcagtgccagaacgtgaattgcagcgccacgttcatcacttatgagtcggtacagcgatacatcgtgaagccgggagaagtccacgccgtaaggccgcacccgttgccatcagggcagcaaattatgtggatgtaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtggcttgccggtctgaggatgagtctcctgtgtcagggctggcacatctgcaatgcgtcgtgttgttgtccggtgtacgtcacaattttcttatactagagtcacacaggaaagtactagatgaatacacaattgatgggtgagcgtattcgcgctcgaagaaaaaaactcaagattagacaagccgctcttggtaagatggtgggagtgtctaatgttgcaatatcgcaatgggagcgctcggagactgagccaaatggggagaacctgttggcactttcgaaggctcttcagtgctcccctgactatttgctgaaaggagatttaagccagacaaacgttgcctatcatagtaggcatgagccaagaggatcataccctcttatcagttgggtaagcgcagggcaatggatggaagctgtagaaccttatcacaagcgcgcgatagagaactggcacgacaccactgtagattgttcagaagattcattttggcttgatgtccaaggtgactctatgacagcaccggcagggttaagcattccagaaggaatgataattctggttgatcccgaagtcgaaccaagaaacggcaagctggttgttgcaaaattagaaggtgaaaacgaggccacattcaaaaaattagttatggatgcaggccgaaagtttttaaaaccattaaacccacaatatccgatgatagaaatcaacggaaactgcaaaatcattggcgtagttgttgacgcaaaactcgcaaatcttccagctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagaataaacttgactaaagattcctttagtagataatttaagtgttctttaatttctactagagttattaagctactaaagcgtagttttcgtcgtttgcagcatttaagactgcttttttaaactgttcattaataggcatagacaatacaaccgatttagtatcacctaatacatgaatttctttgtctccaatacgatgacaaggaactttaatacgctttaaaaatcgctctattgctgttgatgttactgttacatattctgtaataccttgactaacagcgtgtttatatatagcttcaaatagtttcattgtaatttcactagcagagttatttatctttgagctatttttacctacagcaaaacgacttaattcgactatattaggatctttgggagcactctgttgaccaagcaatt…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0032_sequence/1
type: Sequence
persistent identity: BBa B0032 sequence
display id: BBa_B0032_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0084_sequence/1
type: Sequence
persistent identity: BBa R0084 sequence
display id: BBa_R0084_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cttaaattttacttttggttacatattttttctttttgaaaccaaatctttatctttgtagcactttcacggtagcgaaacgttagtttgaatggaaagatgcctgca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K145013_sequence/1
type: Sequence
persistent identity: BBa K145013 sequence
display id: BBa_K145013_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttattaagctactaaagcgtagttttcgtcgtttgcagcatttaagactgcttttttaaactgttcattaataggcatagacaatacaaccgatttagtatcacctaatacatgaatttctttgtctccaatacgatgacaaggaactttaatacgctttaaaaatcgctctattgctgttgatgttactgttacatattctgtaataccttgactaacagcgtgtttatatatagcttcaaatagtttcattgtaatttcactagcagagttatttatctttgagctatttttacctacagcaaaacgacttaattcgactatattaggatctttgggagcactctgttgaccaagcaattcaggaaaaacacttttcagcatataatcacctgttgtaggtaataaacgccagcatccacttacattttcagtatcatcacaagcataaatatattctgcatttgagttatcatactcatctgattcaaggttattttctacaactaagtcccactcaagtctttgcttaaacacttgataacgaagacttagaatacctttatactcctccgatggaattgccaaaaaatccgatttttttatcattatagtcat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0053_sequence/1
type: Sequence
persistent identity: BBa C0053 sequence
display id: BBa_C0053_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaatacacaattgatgggtgagcgtattcgcgctcgaagaaaaaaactcaagattagacaagccgctcttggtaagatggtgggagtgtctaatgttgcaatatcgcaatgggagcgctcggagactgagccaaatggggagaacctgttggcactttcgaaggctcttcagtgctcccctgactatttgctgaaaggagatttaagccagacaaacgttgcctatcatagtaggcatgagccaagaggatcataccctcttatcagttgggtaagcgcagggcaatggatggaagctgtagaaccttatcacaagcgcgcgatagagaactggcacgacaccactgtagattgttcagaagattcattttggcttgatgtccaaggtgactctatgacagcaccggcagggttaagcattccagaaggaatgataattctggttgatcccgaagtcgaaccaagaaacggcaagctggttgttgcaaaattagaaggtgaaaacgaggccacattcaaaaaattagttatggatgcaggccgaaagtttttaaaaccattaaacccacaatatccgatgatagaaatcaacggaaactgcaaaatcattggcgtagttgttgacgcaaaactcgcaaatcttccagctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I746350_sequence/1
type: Sequence
persistent identity: BBa I746350 sequence
display id: BBa_I746350_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgtttcattgtcctttatgccagcatgccgcacatgcgcgtacaagtcgctatatcactgacacgacaaaagagcgttatcatcagtgccagaacgtgaattgcagcgccacgttcatcacttatgagtcggtacagcgatacatcgtgaagccgggagaagtccacgccgtaaggccgcacccgttgccatcagggcagcaaattatgtggatgtaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I746364_sequence/1
type: Sequence
persistent identity: BBa I746364 sequence
display id: BBa_I746364_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tggcttgccggtctgaggatgagtctcctgtgtcagggctggcacatctgcaatgcgtcgtgttgttgtccggtgtacgtcacaattttctta
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0053_sequence/1
type: Sequence
persistent identity: BBa R0053 sequence
display id: BBa_R0053_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aataaacttgactaaagattcctttagtagataatttaagtgttctttaatttc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z