##gff-version 3
##sequence-region BBa_K145113 1 884
BBa_K145113	.	promoter	1	35	.	+	0	ID=BBa_J23116;Name=BBa_J23116
BBa_K145113	.	CDS	63	747	.	+	0	ID=BBa_C0040;Name=BBa_C0040
BBa_K145113	.	CDS	66	682	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_K145113	.	engineered_tag	723	747	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_K145113	.	tag	683	716	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_K145113	.	terminator	756	884	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K145113	.	terminator	844	884	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K145113	.	engineered_region	844	884	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K145113	.	stem_loop	851	870	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K145113	.	polyA_site	871	884	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K145113	.	stop_codon	877	877	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K145113	.	terminator	756	835	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K145113	.	engineered_region	756	835	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K145113	.	stem_loop	767	810	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K145113	.	ribosome_entry_site	44	56	.	+	0	ID=BBa_B0032;Name=BBa_B0032
BBa_K145113	.	ribosome_entry_site	44	56	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K145113	.	engineered_region	44	56	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K145113	.	ribosome_entry_site	50	53	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
>BBa_K145113
ttgacagctagctcagtcctagggactatgctagctactagagtcacacaggaaagtactagatgtccagattagataa
aagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccag
aagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgaga
tgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaag
ttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaa
actctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtgg
ggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactga
tagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggcctt
gaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttag
tagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaa
gactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggc
ctttctgcgtttata