LOCUS       BBa_K145117             2140 bp    DNA     linear       23-Dec-2024
DEFINITION  Lactonase pulse generator
ACCESSION   BBa_K145117
VERSION     BBa_K145117.1  
FEATURES             Location/Qualifiers
     promoter        1..46
                     /label=BBa_I712074
     promoter        92..97
                     /label=-10
     promoter        69..74
                     /label=-35
     misc_feature    79..95
                     /label=OR1
     misc_feature    55..71
                     /label=OR2
     misc_feature    55..103
                     /label=BBa_R0051
     promoter        55..103
                     /label=BBa_R0051
     misc_feature    112..154
                     /label=BBa_J23032
     CDS             164..871
                     /label=cI lambda
     misc_feature    872..904
                     /label=LVA
     misc_feature    911..935
                     /label=Help:Barcodes
     CDS             161..935
                     /label=BBa_C0051
     stem_loop       955..998
                     /label=stem_loop
     misc_feature    944..1023
                     /label=BBa_B0010
     terminator      944..1023
                     /label=BBa_B0010
     stem_loop       1039..1058
                     /label=T7 TE
     misc_feature    1065..1065
                     /label=stop
     polyA_site      1059..1072
                     /label=polya
     misc_feature    1032..1072
                     /label=BBa_B0012
     terminator      1032..1072
                     /label=BBa_B0012
     terminator      944..1072
                     /label=BBa_B0015
     misc_feature    1..1072
                     /label=BBa_K145115
     misc_feature    1089..1105
                     /label=OR1
     misc_feature    1113..1129
                     /label=OR2
     promoter        1128..1133
                     /label=-35
     misc_feature    1139..1145
                     /label=mutate to obliterate OR3
     promoter        1151..1156
                     /label=-10
     promoter        1081..1162
                     /label=BBa_I12007
     RBS             1171..1183
                     /label=RBS-3\Weak
     RBS             1177..1180
                     /label=RBS
     misc_feature    1171..1183
                     /label=BBa_B0032
     RBS             1171..1183
                     /label=BBa_B0032
     misc_feature    1190..1192
                     /label=start
     misc_feature    1973..1978
                     /label=2
     misc_feature    1940..1972
                     /label=LVA
     CDS             1190..1939
                     /label=aiiA
     misc_feature    1190..1978
                     /label=BBa_C0060
     misc_feature    1979..2003
                     /label=Help:Barcodes
     CDS             1190..1978
                     /label=BBa_C0060
     stem_loop       2023..2066
                     /label=stem_loop
     misc_feature    2012..2091
                     /label=BBa_B0010
     terminator      2012..2091
                     /label=BBa_B0010
     stem_loop       2107..2126
                     /label=T7 TE
     misc_feature    2133..2133
                     /label=stop
     polyA_site      2127..2140
                     /label=polya
     misc_feature    2100..2140
                     /label=BBa_B0012
     terminator      2100..2140
                     /label=BBa_B0012
     terminator      2012..2140
                     /label=BBa_B0015
     misc_feature    1081..2140
                     /label=BBa_K145106
ORIGIN
        1 taatacgact cactataggg aatacaagct acttgttctt tttgcatact agagtaacac
       61 cgtgcgtgtt gactatttta cctctggcgg tgataatggt tgctactaga gaactagaat
      121 cacctcttgc ttttgggtaa gacagaagag gagatactag atgagcacaa aaaagaaacc
      181 attaacacaa gagcagcttg aggacgcacg tcgccttaaa gcaatttatg aaaaaaagaa
      241 aaatgaactt ggcttatccc aggaatctgt cgcagacaag atggggatgg ggcagtcagg
      301 cgttggtgct ttatttaatg gcatcaatgc attaaatgct tataacgccg cattgcttgc
      361 aaaaattctc aaagttagcg ttgaagaatt tagcccttca atcgccagag aaatctacga
      421 gatgtatgaa gcggttagta tgcagccgtc acttagaagt gagtatgagt accctgtttt
      481 ttctcatgtt caggcaggga tgttctcacc tgagcttaga acctttacca aaggtgatgc
      541 ggagagatgg gtaagcacaa ccaaaaaagc cagtgattct gcattctggc ttgaggttga
      601 aggtaattcc atgaccgcac caacaggctc caagccaagc tttcctgacg gaatgttaat
      661 tctcgttgac cctgagcagg ctgttgagcc aggtgatttc tgcatagcca gacttggggg
      721 tgatgagttt accttcaaga aactgatcag ggatagcggt caggtgtttt tacaaccact
      781 aaacccacag tacccaatga tcccatgcaa tgagagttgt tccgttgtgg ggaaagttat
      841 cgctagtcag tggcctgaag agacgtttgg cgctgcaaac gacgaaaact acgctttagt
      901 agcttaataa cgctgatagt gctagtgtag atcgctacta gagccaggca tcaaataaaa
      961 cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct
     1021 ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta tatactagag
     1081 gcaaccatta tcaccgccag aggtaaaata gtcaacacgc acggtgttag atatttataa
     1141 atagtggtga tagatttaac gttactagag tcacacagga aagtactaga tgacagtaaa
     1201 gaagctttat ttcgtcccag caggtcgttg tatgttggat cattcgtctg ttaatagtac
     1261 attaacacca ggagaattat tagacttacc ggtttggtgt tatcttttgg agactgaaga
     1321 aggacctatt ttagtagata caggtatgcc agaaagtgca gttaataatg aaggtctttt
     1381 taacggtaca tttgtcgaag ggcaggtttt accgaaaatg actgaagaag atagaatcgt
     1441 gaatatttta aaacgggttg gttatgagcc ggaagacctt ctttatatta ttagttctca
     1501 cttgcatttt gatcatgcag gaggaaatgg cgcttttata aatacaccaa tcattgtaca
     1561 gcgtgctgaa tatgaggcgg cgcagcatag cgaagaatat ttgaaagaat gtatattgcc
     1621 gaatttaaac tacaaaatca ttgaaggtga ttatgaagtc gtaccaggag ttcaattatt
     1681 gcatacacca ggccatactc cagggcatca atcgctatta attgagacag aaaaatccgg
     1741 tcctgtatta ttaacgattg atgcatcgta tacgaaagag aattttgaaa atgaagtgcc
     1801 atttgcggga tttgattcag aattagcttt atcttcaatt aaacgtttaa aagaagtggt
     1861 gatgaaagag aagccgattg ttttctttgg acatgatata gagcaggaaa ggggatgtaa
     1921 agtgttccct gaatatatag ctgcaaacga cgaaaactac gctttagtag cttaataacg
     1981 ctgatagtgc tagtgtagat cgctactaga gccaggcatc aaataaaacg aaaggctcag
     2041 tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt
     2101 cacactggct caccttcggg tgggcctttc tgcgtttata
//