LOCUS       BBa_K1452002            2866 bp    DNA     linear       28-Jun-2024
DEFINITION  Pos/neg selection (Bacillus)
ACCESSION   BBa_K1452002
VERSION     BBa_K1452002.1  
FEATURES             Location/Qualifiers
     CDS             31..1113
                     /label=lacI
     CDS             1363..1698
                     /label=mazF
     promoter        1715..2003
                     /label=Pspac
     CDS             2113..2865
                     /label=SpcR
     misc_feature    1722..1738
                     /label=lac operator
ORIGIN
        1 ggatcctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
       61 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
      121 cgccagggtg gtttttcttt tcaccagtga gacgggcaac agctgattgc ccttcaccgc
      181 ctggccctga gagagttgca gcaagcggtc cacgctggtt tgccccagca ggcgaaaatc
      241 ctgtttgatg gtggttgacg gcgggatata acatgagctg tcttcggtat cgtcgtatcc
      301 cactaccgag atatccgcac caacgcgcag cccggactcg gtaatggcgc gcattgcgcc
      361 cagcgccatc tgatcgttgg caaccagcat cgcagtggga acgatgccct cattcagcat
      421 ttgcatggtt tgttgaaaac cggacatggc actccagtcg ccttcccgtt ccgctatcgg
      481 ctgaatttga ttgcgagtga gatatttatg ccagccagcc agacgcagac gcgccgagac
      541 agaacttaat gggcccgcta acagcgcgat ttgctggtga cccaatgcga ccagatgctc
      601 cacgcccagt cgcgtaccgt cttcatggga gaaaataata ctgttgatgg gtgtctggtc
      661 agagacatca agaaataacg ccggaacatt agtgcaggca gcttccacag caatggcatc
      721 ctggtcatcc agcggatagt taatgatcag cccactgacg cgttgcgcga gaagattgtg
      781 caccgccgct ttacaggctt cgacgccgct tcgttctacc atcgacacca ccacgctggc
      841 acccagttga tcggcgcgag atttaatcgc cgcgacaatt tgcgacggcg cgtgcagggc
      901 cagactggag gtggcaacgc caatcagcaa cgactgtttg cccgccagtt gttgtgccac
      961 gcggttggga atgtaattca gctccgccat cgccgcttcc actttttccc gcgttttcgc
     1021 agaaacgtgg ctggcctggt tcaccacgcg ggaaacggtc tgataagaga caccggcata
     1081 ctctgcgaca tcgtataacg ttactggttt catcaaaatc gtctccctcc gtttgaatat
     1141 ttgattgatc gtaaccagat gaagcactct ttccactatc cctacagtgt tatggcttga
     1201 acaatcacga aacaataatt ggtacgtacg atctttcagc cgactcaaac atcaaatctt
     1261 acaaatgtag tctttgaaag tattacatat gtaagattta aatgcaaccg ttttttcgga
     1321 aggaaatgat gacctcgttt ccaccggaat tagcttgcat gcctacccaa tcagtacgtt
     1381 aattttggct ttaatgagtt gtaattcctc tggggcaact gttcctttct tcgttgctcc
     1441 tcttgcccgc caggcgatac tttttacctg atcagctaac gctacgccat cacgttcctg
     1501 accggataaa acaacttcga acggatatcc ttttgattgc gttgtacaag gaacacacag
     1561 acacatacct gttttgttgt tgtacatgaa aggactcagg acaacagctg gacgatgtcc
     1621 agcttgctcg ctaccttttg tcgggtcaaa atcaacccaa atcagatcgc ccatatcggg
     1681 tacgtatcgg cttaccatac ctgcttcctc cttaagctta attgttatcc gctcacaatt
     1741 ccacacatta tgccacacct tgtagataaa gtcaacaact ttttgcaaaa tttttcagga
     1801 attttagcag aggttgttct ggatgtagaa caaaacatct ttccgctctt gtgctgttag
     1861 gatatctttc ttggaagcta ggtaggcaag ggctacctca aataaatctt cttcagggtg
     1921 cgctattttt aaggtgccta gtgaggtctt gaccacttca cccataattt cagtgccgaa
     1981 tagtctggac tgggctgtgt agataaaaaa tttagaagcc aatgaaatct ataaataaac
     2041 taaattaagt ttatttaatt aacaactatg gatataaaat aggtactaat caaaatagtg
     2101 aggaggatat atttgaatac atacgaacaa attaataaag tgaaaaaaat acttcggaaa
     2161 catttaaaaa ataaccttat tggtacttac atgtttggat caggagttga gagtggacta
     2221 aaaccaaata gtgatcttga ctttttagtc gtcgtatctg aaccattgac agatcaaagt
     2281 aaagaaatac ttatacaaaa aattagacct atttcaaaaa aaataggaga taaaagcaac
     2341 ttacgatata ttgaattaac aattattatt cagcaagaaa tggtaccgtg gaatcatcct
     2401 cccaaacaag aatttattta tggagaatgg ttacaagagc tttatgaaca aggatacatt
     2461 cctcagaagg aattaaattc agatttaacc ataatgcttt accaagcaaa acgaaaaaat
     2521 aaaagaatat acggaaatta tgacttagag gaattactac ctgatattcc attttctgat
     2581 gtgagaagag ccattatgga ttcgtcagag gaattaatag ataattatca ggatgatgaa
     2641 accaactcta tattaacttt atgccgtatg attttaacta tggacacggg taaaatcata
     2701 ccaaaagata ttgcgggaaa tgcagtggct gaatcttctc cattagaaca tagggagaga
     2761 attttgttag cagttcgtag ttatcttgga gagaatattg aatggactaa tgaaaatgta
     2821 aatttaacta taaactattt aaataacaga ttaaaaaaat tataaa
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