LOCUS       BBa_K145205              977 bp    DNA     linear       29-Dec-2025
DEFINITION  OUTPUT GFP under TetR pomoter
ACCESSION   BBa_K145205
VERSION     BBa_K145205.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     RBS             63..75
                     /label=RBS-3\Weak
     RBS             69..72
                     /label=RBS
     misc_feature    63..75
                     /label=BBa_B0032
     RBS             63..75
                     /label=BBa_B0032
     misc_feature    82..84
                     /label=start
     misc_feature    835..837
                     /label=stop
     misc_feature    838..840
                     /label=stop
     misc_feature    798..834
                     /label=LVA tag
     CDS             85..797
                     /label=cds
     misc_feature    82..840
                     /label=BBa_K145015
     stem_loop       860..903
                     /label=stem_loop
     misc_feature    849..928
                     /label=BBa_B0010
     terminator      849..928
                     /label=BBa_B0010
     stem_loop       944..963
                     /label=T7 TE
     misc_feature    970..970
                     /label=stop
     polyA_site      964..977
                     /label=polya
     misc_feature    937..977
                     /label=BBa_B0012
     terminator      937..977
                     /label=BBa_B0012
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agtcacacag gaaagtacta gatgcgtaaa ggagaagaac ttttcactgg agttgtccca
      121 attcttgttg aattagatgg tgatgttaat gggcacaaat tttctgtcag tggagagggt
      181 gaaggtgatg caacatacgg aaaacttacc cttaaattta tttgcactac tggaaaacta
      241 cctgttccat ggccaacact tgtcactact ttcggttatg gtgttcaatg ctttgcgaga
      301 tacccagatc atatgaaaca gcatgacttt ttcaagagtg ccatgcccga aggttatgta
      361 caggaaagaa ctatattttt caaagatgac gggaactaca agacacgtgc tgaagtcaag
      421 tttgaaggtg atacccttgt taatagaatc gagttaaaag gtattgattt taaagaagat
      481 ggaaacattc ttggacacaa attggaatac aactataact cacacaatgt atacatcatg
      541 gcagacaaac aaaagaatgg aatcaaagtt aacttcaaaa ttagacacaa cattgaagat
      601 ggaagcgttc aactagcaga ccattatcaa caaaatactc caattggcga tggccctgtc
      661 cttttaccag acaaccatta cctgtccaca caatctgccc tttcgaaaga tcccaacgaa
      721 aagagagacc acatggtcct tcttgagttt gtaacagctg ctgggattac acatggcatg
      781 gatgaactat acaaaaggcc tgctgcaaac gacgaaaact acgctttagt agcttaataa
      841 tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt
      901 tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg
      961 gcctttctgc gtttata
//