##gff-version 3
##sequence-region BBa_K145205 1 977
BBa_K145205	.	terminator	937	977	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K145205	.	stem_loop	944	963	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K145205	.	engineered_region	937	977	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K145205	.	stop_codon	970	970	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K145205	.	polyA_site	964	977	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K145205	.	terminator	849	928	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K145205	.	engineered_region	849	928	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K145205	.	stem_loop	860	903	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K145205	.	ribosome_entry_site	63	75	.	+	0	ID=BBa_B0032;Name=BBa_B0032
BBa_K145205	.	engineered_region	63	75	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K145205	.	ribosome_entry_site	69	72	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K145205	.	ribosome_entry_site	63	75	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K145205	.	engineered_region	82	840	.	+	0	ID=BBa_K145015;Name=BBa_K145015
BBa_K145205	.	CDS	85	797	.	+	0	ID=annotation1969620;Name=cds;Parent=BBa_K145015
BBa_K145205	.	stop_codon	838	840	.	+	0	ID=annotation1969618;Name=stop;Parent=BBa_K145015
BBa_K145205	.	start_codon	82	84	.	+	0	ID=annotation1969616;Name=start;Parent=BBa_K145015
BBa_K145205	.	tag	798	834	.	+	0	ID=annotation1969619;Name=LVA tag;Parent=BBa_K145015
BBa_K145205	.	stop_codon	835	837	.	+	0	ID=annotation1969617;Name=stop;Parent=BBa_K145015
BBa_K145205	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_K145205	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K145205	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K145205	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K145205	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K145205	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
>BBa_K145205
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagtcacacaggaaagtact
agatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaa
attttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaa
ctacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatga
aacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaa
ctacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaa
gaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaa
agaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaaca
aaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagat
cccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactat
acaaaaggcctgctgcaaacgacgaaaactacgctttagtagcttaataatactagagccaggcatcaaataaaacgaa
aggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctc
accttcgggtgggcctttctgcgtttata