##gff-version 3
##sequence-region BBa_K145210 1 2701
BBa_K145210	.	promoter	1782	1827	.	+	0	ID=BBa_R1052;Name=BBa_R1052
BBa_K145210	.	promoter	1783	1788	.	+	0	ID=annotation2083;Name=-35;Parent=BBa_R1052
BBa_K145210	.	promoter	1782	1787	.	+	0	ID=annotation2082;Name=-35;Parent=BBa_R1052
BBa_K145210	.	promoter	1782	1824	.	+	0	ID=annotation2079;Name=-10;Parent=BBa_R1052
BBa_K145210	.	start_codon	1816	1818	.	+	0	ID=annotation2084;Name=start;Parent=BBa_R1052
BBa_K145210	.	non_covalent_binding_site	1789	1802	.	+	0	ID=annotation2080;Name=OR2;Parent=BBa_R1052
BBa_K145210	.	engineered_region	1782	1827	.	+	0	ID=annotation7075;Name=BBa_R1052;Parent=BBa_R1052
BBa_K145210	.	promoter	1805	1810	.	+	0	ID=annotation2087;Name=-10;Parent=BBa_R1052
BBa_K145210	.	non_covalent_binding_site	1811	1824	.	+	0	ID=annotation2081;Name=OR1;Parent=BBa_R1052
BBa_K145210	.	engineered_region	1836	2701	.	+	0	ID=BBa_P0353;Name=BBa_P0353
BBa_K145210	.	CDS	1853	2539	.	+	0	ID=BBa_C0053;Name=BBa_C0053;Parent=BBa_P0353
BBa_K145210	.	stop_codon	2534	2539	.	+	0	ID=annotation1751;Name=stop;Parent=BBa_C0053
BBa_K145210	.	engineered_tag	2540	2564	.	+	0	ID=annotation2213993;Name=Help:Barcodes;Parent=BBa_C0053
BBa_K145210	.	tag	2501	2533	.	+	0	ID=annotation1750;Name=LVA;Parent=BBa_C0053
BBa_K145210	.	CDS	1853	2500	.	+	0	ID=annotation1747;Name=cII p22;Parent=BBa_C0053
BBa_K145210	.	engineered_region	1853	2539	.	+	0	ID=annotation7036;Name=BBa_C0053;Parent=BBa_C0053
BBa_K145210	.	terminator	2573	2652	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_P0353
BBa_K145210	.	stem_loop	2584	2627	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K145210	.	engineered_region	2573	2652	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K145210	.	ribosome_entry_site	1836	1846	.	+	0	ID=BBa_B0033;Name=BBa_B0033;Parent=BBa_P0353
BBa_K145210	.	engineered_region	1836	1846	.	+	0	ID=annotation7028;Name=BBa_B0033;Parent=BBa_B0033
BBa_K145210	.	ribosome_entry_site	1842	1845	.	+	0	ID=annotation1714;Name=RBS;Parent=BBa_B0033
BBa_K145210	.	ribosome_entry_site	1836	1846	.	+	0	ID=annotation1713;Name=RBS-4\Weaker;Parent=BBa_B0033
BBa_K145210	.	terminator	2661	2701	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_P0353
BBa_K145210	.	polyA_site	2688	2701	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K145210	.	stem_loop	2668	2687	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K145210	.	stop_codon	2694	2694	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K145210	.	engineered_region	2661	2701	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K145210	.	engineered_region	923	1773	.	+	0	ID=BBa_P0152;Name=BBa_P0152
BBa_K145210	.	ribosome_entry_site	923	936	.	+	0	ID=BBa_B0031;Name=BBa_B0031;Parent=BBa_P0152
BBa_K145210	.	conserved_region	929	932	.	+	0	ID=annotation23316;Name=conserved;Parent=BBa_B0031
BBa_K145210	.	CDS	943	1611	.	+	0	ID=BBa_C0052;Name=BBa_C0052;Parent=BBa_P0152
BBa_K145210	.	engineered_tag	1612	1636	.	+	0	ID=annotation2213992;Name=Help:Barcodes;Parent=BBa_C0052
BBa_K145210	.	CDS	943	1611	.	+	0	ID=annotation1743;Name=cI 434;Parent=BBa_C0052
BBa_K145210	.	tag	1573	1611	.	+	0	ID=annotation1745;Name=LVA;Parent=BBa_C0052
BBa_K145210	.	engineered_region	943	1611	.	+	0	ID=annotation7035;Name=BBa_C0052;Parent=BBa_C0052
BBa_K145210	.	terminator	1645	1724	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_P0152
BBa_K145210	.	stem_loop	1656	1699	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K145210	.	engineered_region	1645	1724	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K145210	.	terminator	1733	1773	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_P0152
BBa_K145210	.	polyA_site	1760	1773	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K145210	.	stem_loop	1740	1759	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K145210	.	stop_codon	1766	1766	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K145210	.	engineered_region	1733	1773	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K145210	.	terminator	724	852	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K145210	.	terminator	812	852	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K145210	.	polyA_site	839	852	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K145210	.	stem_loop	819	838	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K145210	.	stop_codon	845	845	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K145210	.	engineered_region	812	852	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K145210	.	terminator	724	803	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K145210	.	stem_loop	735	778	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K145210	.	engineered_region	724	803	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K145210	.	promoter	861	914	.	+	0	ID=BBa_R0053;Name=BBa_R0053
BBa_K145210	.	non_covalent_binding_site	894	911	.	+	0	ID=annotation2037;Name=OR1;Parent=BBa_R0053
BBa_K145210	.	non_covalent_binding_site	871	888	.	+	0	ID=annotation2036;Name=OR2;Parent=BBa_R0053
BBa_K145210	.	promoter	890	895	.	+	0	ID=annotation2042;Name=-10;Parent=BBa_R0053
BBa_K145210	.	non_covalent_binding_site	861	863	.	+	0	ID=annotation2035;Name=OR3;Parent=BBa_R0053
BBa_K145210	.	engineered_region	861	914	.	+	0	ID=annotation7069;Name=BBa_R0053;Parent=BBa_R0053
BBa_K145210	.	promoter	878	883	.	+	0	ID=annotation2038;Name=-35;Parent=BBa_R0053
BBa_K145210	.	promoter	868	873	.	+	0	ID=annotation2041;Name=-35;Parent=BBa_R0053
BBa_K145210	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_K145210	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K145210	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K145210	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K145210	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K145210	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K145210	.	ribosome_entry_site	63	73	.	+	0	ID=BBa_B0033;Name=BBa_B0033
BBa_K145210	.	engineered_region	63	73	.	+	0	ID=annotation7028;Name=BBa_B0033;Parent=BBa_B0033
BBa_K145210	.	ribosome_entry_site	69	72	.	+	0	ID=annotation1714;Name=RBS;Parent=BBa_B0033
BBa_K145210	.	ribosome_entry_site	63	73	.	+	0	ID=annotation1713;Name=RBS-4\Weaker;Parent=BBa_B0033
BBa_K145210	.	CDS	80	715	.	+	0	ID=BBa_C0056;Name=BBa_C0056
BBa_K145210	.	stop_codon	710	715	.	+	0	ID=annotation1063930;Name=2;Parent=BBa_C0056
BBa_K145210	.	CDS	80	715	.	+	0	ID=annotation1063931;Name=cI 434;Parent=BBa_C0056
>BBa_K145210
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagtcacacaggactactag
atgagtatttcttccagggtaaaaagcaaaagaatccagcttggacttaaccaggctgaacttgctcaaaaggtgggga
ctacccagcagtctatagagcagctcgaaaacggtaaaactaagcgaccacgctttttaccagaacttgcgtcagctct
tggcgtaagtgttgactggctgctcaatggcacctctgattcgaatgttagatttgttgggcacgttgagcccaaaggg
aaatatccattgattagcatggttagagctggttcgtggtgtgaagcttgtgaaccctacgatatcaaggacattgatg
aatggtatgacagtgacgttaacttattaggcaatggattctggctgaaggttgaaggtgattccatgacctcacctgt
aggtcaaagcatccctgaaggtcatatggtgttagtagatactggacgggagccagtgaatggaagccttgttgtagcc
aaactgactgacgcgaacgaagcaacattcaagaaactggtcatagatggcggtcagaagtacctgaaaggcctgaatc
cttcatggcctatgactcctatcaacggaaactgcaagattatcggtgttgtcgtggaagcgagggtaaaattcgtata
ataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtc
ggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagaataaactt
gactaaagattcctttagtagataatttaagtgttctttaatttctactagagtcacacaggaaacctactagatgagt
atttcttccagggtaaaaagcaaaagaatccagcttggacttaaccaggctgaacttgctcaaaaggtggggactaccc
agcagtctatagagcagctcgaaaacggtaaaactaagcgaccacgctttttaccagaacttgcgtcagctcttggcgt
aagtgttgactggctgctcaatggcacctctgattcgaatgttagatttgttgggcacgttgagcccaaagggaaatat
ccattgattagcatggttagagctggttcgtggtgtgaagcttgtgaaccctacgatatcaaggacattgatgaatggt
atgacagtgacgttaacttattaggcaatggattctggctgaaggttgaaggtgattccatgacctcacctgtaggtca
aagcatccctgaaggtcatatggtgttagtagatactggacgggagccagtgaatggaagccttgttgtagccaaactg
actgacgcgaacgaagcaacattcaagaaactggtcatagatggcggtcagaagtacctgaaaggcctgaatccttcat
ggcctatgactcctatcaacggaaactgcaagattatcggtgttgtcgtggaagcgagggtaaaattcgtagctgcaaa
cgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagccaggcatcaaataa
aacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacac
tggctcaccttcgggtgggcctttctgcgtttatatactagagttgacaaacaagatacattgtatgatactacaagaa
agtttgttgatactagagtcacacaggactactagatgaatacacaattgatgggtgagcgtattcgcgctcgaagaaa
aaaactcaagattagacaagccgctcttggtaagatggtgggagtgtctaatgttgcaatatcgcaatgggagcgctcg
gagactgagccaaatggggagaacctgttggcactttcgaaggctcttcagtgctcccctgactatttgctgaaaggag
atttaagccagacaaacgttgcctatcatagtaggcatgagccaagaggatcataccctcttatcagttgggtaagcgc
agggcaatggatggaagctgtagaaccttatcacaagcgcgcgatagagaactggcacgacaccactgtagattgttca
gaagattcattttggcttgatgtccaaggtgactctatgacagcaccggcagggttaagcattccagaaggaatgataa
ttctggttgatcccgaagtcgaaccaagaaacggcaagctggttgttgcaaaattagaaggtgaaaacgaggccacatt
caaaaaattagttatggatgcaggccgaaagtttttaaaaccattaaacccacaatatccgatgatagaaatcaacgga
aactgcaaaatcattggcgtagttgttgacgcaaaactcgcaaatcttccagctgcaaacgacgaaaactacgctttag
tagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaa
gactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggc
ctttctgcgtttata