LOCUS       BBa_K145216             2980 bp    DNA     linear       21-May-2024
DEFINITION  FILTER T7 RNA pol
ACCESSION   BBa_K145216
VERSION     BBa_K145216.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23109
     misc_feature    44..95
                     /label=BBa_J23078
     misc_feature    102..104
                     /label=start
     misc_feature    2841..2843
                     /label=stop
     misc_feature    2838..2840
                     /label=stop
     misc_feature    102..188
                     /label=tag
     CDS             189..2837
                     /label=cds
     CDS             102..2843
                     /label=BBa_K145014
     stem_loop       2863..2906
                     /label=stem_loop
     misc_feature    2852..2931
                     /label=BBa_B0010
     terminator      2852..2931
                     /label=BBa_B0010
     stem_loop       2947..2966
                     /label=T7 TE
     misc_feature    2973..2973
                     /label=stop
     polyA_site      2967..2980
                     /label=polya
     misc_feature    2940..2980
                     /label=BBa_B0012
     terminator      2940..2980
                     /label=BBa_B0012
ORIGIN
        1 tttacagcta gctcagtcct agggactgtg ctagctacta gagtgtagac cgaactagaa
       61 tcacctcttg cttttgggta agacagaaga ggagatacta gatgttgttt atcaagcctg
      121 cggatctccg cgaaattgtg acttttccgc tatttagcga tcttgttcag tgtggctttc
      181 cttcaccgat gaacacgatt aacatcgcta agaacgactt ctctgacatc gaactggctg
      241 ctatcccgtt caacactctg gctgaccatt acggtgagcg tttagctcgc gaacagttgg
      301 cccttgagca tgagtcttac gagatgggtg aagcacgctt ccgcaagatg tttgagcgtc
      361 aacttaaagc tggtgaggtt gcggataacg ctgccgccaa gcctctcatc actaccctac
      421 tccctaagat gattgcacgc atcaacgact ggtttgagga agtgaaagct aagcgcggca
      481 agcgcccgac agccttccag ttcctgcaag aaatcaagcc ggaagccgta gcgtacatca
      541 ccattaagac cactctggct tgcctaacca gtgctgacaa tacaaccgtt caggctgtag
      601 caagcgcaat cggtcgggcc attgaggacg aggctcgctt cggtcgtatc cgtgaccttg
      661 aagctaagca cttcaagaaa aacgttgagg aacaactcaa caagcgcgta gggcacgtct
      721 acaagaaagc atttatgcaa gttgtcgagg ctgacatgct ctctaagggt ctactcggtg
      781 gcgaggcgtg gtcttcgtgg cataaggaag actctattca tgtaggagta cgctgcatcg
      841 agatgctcat tgagtcaacc ggaatggtta gcttacaccg ccaaaatgct ggcgtagtag
      901 gtcaagactc tgagactatc gaactcgcac ctgaatacgc tgaggctatc gcaacccgtg
      961 caggtgcgct ggctggcatc tctccgatgt tccaaccttg cgtagttcct cctaagccgt
     1021 ggactggcat tactggtggt ggctattggg ctaacggtcg tcgtcctctg gcgctggtgc
     1081 gtactcacag taagaaagca ctgatgcgct acgaagacgt ttacatgcct gaggtgtaca
     1141 aagcgattaa cattgcgcaa aacaccgcat ggaaaatcaa caagaaagtc ctagcggtcg
     1201 ccaacgtaat caccaagtgg aagcattgtc cggtcgagga catccctgcg attgagcgtg
     1261 aagaactccc gatgaaaccg gaagacatcg acatgaatcc tgaggctctc accgcgtgga
     1321 aacgtgctgc cgctgctgtg taccgcaagg acaaggctcg caagtctcgc cgtatcagcc
     1381 ttgagttcat gcttgagcaa gccaataagt ttgctaacca taaggccatc tggttccctt
     1441 acaacatgga ctggcgcggt cgtgtttacg ctgtgtcaat gttcaacccg caaggtaacg
     1501 atatgaccaa aggactgctt acgctggcga aaggtaaacc aatcggtaag gaaggttact
     1561 actggctgaa aatccacggt gcaaactgtg cgggtgtcga taaggttccg ttccctgagc
     1621 gcatcaagtt cattgaggaa aaccacgaga acatcatggc ttgcgctaag tctccactgg
     1681 agaacacttg gtgggctgag caagattctc cgttctgctt ccttgcgttc tgctttgagt
     1741 acgctggggt acagcaccac ggcctgagct ataactgctc ccttccgctg gcgtttgacg
     1801 ggtcttgctc tggcatccag cacttctccg cgatgctccg agatgaggta ggtggtcgcg
     1861 cggttaactt gcttcctagt gaaaccgttc aggacatcta cgggattgtt gctaagaaag
     1921 tcaacgagat tctacaagca gacgcaatca atgggaccga taacgaagta gttaccgtga
     1981 ccgatgagaa cactggtgaa atctctgaga aagtcaagct gggcactaag gcactggctg
     2041 gtcaatggct ggcttacggt gttactcgca gtgtgactaa gcgttcagtc atgacgctgg
     2101 cttacgggtc caaagagttc ggcttccgtc aacaagtgct ggaagatacc attcagccag
     2161 ctattgattc cggcaagggt ctgatgttca ctcagccgaa tcaggctgct ggatacatgg
     2221 ctaagctgat ttgggaatct gtgagcgtga cggtggtagc tgcggttgaa gcaatgaact
     2281 ggcttaagtc tgctgctaag ctgctggctg ctgaggtcaa agataagaag actggagaga
     2341 ttcttcgcaa gcgttgcgct gtgcattggg taactcctga tggtttccct gtgtggcagg
     2401 aatacaagaa gcctattcag acgcgcttga acctgatgtt cctcggtcag ttccgcttac
     2461 agcctaccat taacaccaac aaagatagcg agattgatgc acacaaacag gagtctggta
     2521 tcgctcctaa ctttgtacac agccaagacg gtagccacct tcgtaagact gtagtgtggg
     2581 cacacgagaa gtacggaatc gaatcttttg cactgattca cgactccttc ggtaccattc
     2641 cggctgacgc tgcgaacctg ttcaaagcag tgcgcgaaac tatggttgac acatatgagt
     2701 cttgtgatgt actggctgat ttctacgacc agttcgctga ccagttgcac gagtctcaat
     2761 tggacaaaat gccagcactt ccggctaaag gtaacttgaa cctccgtgac atcttagagt
     2821 cggacttcgc gttcgcgtaa taatactaga gccaggcatc aaataaaacg aaaggctcag
     2881 tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt
     2941 cacactggct caccttcggg tgggcctttc tgcgtttata
//