LOCUS       BBa_K145217             3386 bp    DNA     linear       17-May-2024
DEFINITION  FILTER Complete
ACCESSION   BBa_K145217
VERSION     BBa_K145217.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    63..156
                     /label=BBa_J23008
     misc_feature    165..261
                     /label=BBa_J23009
     stem_loop       281..324
                     /label=stem_loop
     misc_feature    270..349
                     /label=BBa_B0010
     terminator      270..349
                     /label=BBa_B0010
     stem_loop       365..384
                     /label=T7 TE
     misc_feature    391..391
                     /label=stop
     polyA_site      385..398
                     /label=polya
     misc_feature    358..398
                     /label=BBa_B0012
     terminator      358..398
                     /label=BBa_B0012
     promoter        407..441
                     /label=BBa_J23109
     misc_feature    450..501
                     /label=BBa_J23078
     misc_feature    508..510
                     /label=start
     misc_feature    3247..3249
                     /label=stop
     misc_feature    3244..3246
                     /label=stop
     misc_feature    508..594
                     /label=tag
     CDS             595..3243
                     /label=cds
     CDS             508..3249
                     /label=BBa_K145014
     stem_loop       3269..3312
                     /label=stem_loop
     misc_feature    3258..3337
                     /label=BBa_B0010
     terminator      3258..3337
                     /label=BBa_B0010
     stem_loop       3353..3372
                     /label=T7 TE
     misc_feature    3379..3379
                     /label=stop
     polyA_site      3373..3386
                     /label=polya
     misc_feature    3346..3386
                     /label=BBa_B0012
     terminator      3346..3386
                     /label=BBa_B0012
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agacccaaaa gcaagaggtg attctagttg gtggttaatg aaaattaact tacttactag
      121 aaatatctct aaaaagccag attattaatc cggctttact agagcgggcg ggcgggcggg
      181 cgggatccat aaaaaaaaaa cccaaaagca agaggtgatt ctagttaaaa aaaaaaagct
      241 tcccgcccgc ccgcccgccc gtactagagc caggcatcaa ataaaacgaa aggctcagtc
      301 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca
      361 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagttta cagctagctc
      421 agtcctaggg actgtgctag ctactagagt gtagaccgaa ctagaatcac ctcttgcttt
      481 tgggtaagac agaagaggag atactagatg ttgtttatca agcctgcgga tctccgcgaa
      541 attgtgactt ttccgctatt tagcgatctt gttcagtgtg gctttccttc accgatgaac
      601 acgattaaca tcgctaagaa cgacttctct gacatcgaac tggctgctat cccgttcaac
      661 actctggctg accattacgg tgagcgttta gctcgcgaac agttggccct tgagcatgag
      721 tcttacgaga tgggtgaagc acgcttccgc aagatgtttg agcgtcaact taaagctggt
      781 gaggttgcgg ataacgctgc cgccaagcct ctcatcacta ccctactccc taagatgatt
      841 gcacgcatca acgactggtt tgaggaagtg aaagctaagc gcggcaagcg cccgacagcc
      901 ttccagttcc tgcaagaaat caagccggaa gccgtagcgt acatcaccat taagaccact
      961 ctggcttgcc taaccagtgc tgacaataca accgttcagg ctgtagcaag cgcaatcggt
     1021 cgggccattg aggacgaggc tcgcttcggt cgtatccgtg accttgaagc taagcacttc
     1081 aagaaaaacg ttgaggaaca actcaacaag cgcgtagggc acgtctacaa gaaagcattt
     1141 atgcaagttg tcgaggctga catgctctct aagggtctac tcggtggcga ggcgtggtct
     1201 tcgtggcata aggaagactc tattcatgta ggagtacgct gcatcgagat gctcattgag
     1261 tcaaccggaa tggttagctt acaccgccaa aatgctggcg tagtaggtca agactctgag
     1321 actatcgaac tcgcacctga atacgctgag gctatcgcaa cccgtgcagg tgcgctggct
     1381 ggcatctctc cgatgttcca accttgcgta gttcctccta agccgtggac tggcattact
     1441 ggtggtggct attgggctaa cggtcgtcgt cctctggcgc tggtgcgtac tcacagtaag
     1501 aaagcactga tgcgctacga agacgtttac atgcctgagg tgtacaaagc gattaacatt
     1561 gcgcaaaaca ccgcatggaa aatcaacaag aaagtcctag cggtcgccaa cgtaatcacc
     1621 aagtggaagc attgtccggt cgaggacatc cctgcgattg agcgtgaaga actcccgatg
     1681 aaaccggaag acatcgacat gaatcctgag gctctcaccg cgtggaaacg tgctgccgct
     1741 gctgtgtacc gcaaggacaa ggctcgcaag tctcgccgta tcagccttga gttcatgctt
     1801 gagcaagcca ataagtttgc taaccataag gccatctggt tcccttacaa catggactgg
     1861 cgcggtcgtg tttacgctgt gtcaatgttc aacccgcaag gtaacgatat gaccaaagga
     1921 ctgcttacgc tggcgaaagg taaaccaatc ggtaaggaag gttactactg gctgaaaatc
     1981 cacggtgcaa actgtgcggg tgtcgataag gttccgttcc ctgagcgcat caagttcatt
     2041 gaggaaaacc acgagaacat catggcttgc gctaagtctc cactggagaa cacttggtgg
     2101 gctgagcaag attctccgtt ctgcttcctt gcgttctgct ttgagtacgc tggggtacag
     2161 caccacggcc tgagctataa ctgctccctt ccgctggcgt ttgacgggtc ttgctctggc
     2221 atccagcact tctccgcgat gctccgagat gaggtaggtg gtcgcgcggt taacttgctt
     2281 cctagtgaaa ccgttcagga catctacggg attgttgcta agaaagtcaa cgagattcta
     2341 caagcagacg caatcaatgg gaccgataac gaagtagtta ccgtgaccga tgagaacact
     2401 ggtgaaatct ctgagaaagt caagctgggc actaaggcac tggctggtca atggctggct
     2461 tacggtgtta ctcgcagtgt gactaagcgt tcagtcatga cgctggctta cgggtccaaa
     2521 gagttcggct tccgtcaaca agtgctggaa gataccattc agccagctat tgattccggc
     2581 aagggtctga tgttcactca gccgaatcag gctgctggat acatggctaa gctgatttgg
     2641 gaatctgtga gcgtgacggt ggtagctgcg gttgaagcaa tgaactggct taagtctgct
     2701 gctaagctgc tggctgctga ggtcaaagat aagaagactg gagagattct tcgcaagcgt
     2761 tgcgctgtgc attgggtaac tcctgatggt ttccctgtgt ggcaggaata caagaagcct
     2821 attcagacgc gcttgaacct gatgttcctc ggtcagttcc gcttacagcc taccattaac
     2881 accaacaaag atagcgagat tgatgcacac aaacaggagt ctggtatcgc tcctaacttt
     2941 gtacacagcc aagacggtag ccaccttcgt aagactgtag tgtgggcaca cgagaagtac
     3001 ggaatcgaat cttttgcact gattcacgac tccttcggta ccattccggc tgacgctgcg
     3061 aacctgttca aagcagtgcg cgaaactatg gttgacacat atgagtcttg tgatgtactg
     3121 gctgatttct acgaccagtt cgctgaccag ttgcacgagt ctcaattgga caaaatgcca
     3181 gcacttccgg ctaaaggtaa cttgaacctc cgtgacatct tagagtcgga cttcgcgttc
     3241 gcgtaataat actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc
     3301 ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc
     3361 ttcgggtggg cctttctgcg tttata
//