##gff-version 3
##sequence-region BBa_K145217 1 3386
BBa_K145217	.	terminator	3346	3386	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K145217	.	polyA_site	3373	3386	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K145217	.	stem_loop	3353	3372	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K145217	.	engineered_region	3346	3386	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K145217	.	stop_codon	3379	3379	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K145217	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_K145217	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K145217	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K145217	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K145217	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K145217	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K145217	.	mature_transcript_region	165	261	.	+	0	ID=BBa_J23009;Name=BBa_J23009
BBa_K145217	.	mature_transcript_region	63	156	.	+	0	ID=BBa_J23008;Name=BBa_J23008
BBa_K145217	.	mature_transcript_region	450	501	.	+	0	ID=BBa_J23078;Name=BBa_J23078
BBa_K145217	.	promoter	407	441	.	+	0	ID=BBa_J23109;Name=BBa_J23109
BBa_K145217	.	terminator	270	349	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K145217	.	stem_loop	281	324	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K145217	.	engineered_region	270	349	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K145217	.	terminator	3258	3337	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K145217	.	stem_loop	3269	3312	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K145217	.	engineered_region	3258	3337	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K145217	.	CDS	508	3249	.	+	0	ID=BBa_K145014;Name=BBa_K145014
BBa_K145217	.	tag	508	594	.	+	0	ID=annotation1968241;Name=tag;Parent=BBa_K145014
BBa_K145217	.	stop_codon	3247	3249	.	+	0	ID=annotation1968239;Name=stop;Parent=BBa_K145014
BBa_K145217	.	stop_codon	3244	3246	.	+	0	ID=annotation1968240;Name=stop;Parent=BBa_K145014
BBa_K145217	.	start_codon	508	510	.	+	0	ID=annotation1968238;Name=start;Parent=BBa_K145014
BBa_K145217	.	CDS	595	3243	.	+	0	ID=annotation1968242;Name=cds;Parent=BBa_K145014
BBa_K145217	.	terminator	358	398	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K145217	.	polyA_site	385	398	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K145217	.	stem_loop	365	384	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K145217	.	engineered_region	358	398	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K145217	.	stop_codon	391	391	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
>BBa_K145217
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagacccaaaagcaagaggt
gattctagttggtggttaatgaaaattaacttacttactagaaatatctctaaaaagccagattattaatccggcttta
ctagagcgggcgggcgggcgggcgggatccataaaaaaaaaacccaaaagcaagaggtgattctagttaaaaaaaaaaa
gcttcccgcccgcccgcccgcccgtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggccttt
cgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgttt
atatactagagtttacagctagctcagtcctagggactgtgctagctactagagtgtagaccgaactagaatcacctct
tgcttttgggtaagacagaagaggagatactagatgttgtttatcaagcctgcggatctccgcgaaattgtgacttttc
cgctatttagcgatcttgttcagtgtggctttccttcaccgatgaacacgattaacatcgctaagaacgacttctctga
catcgaactggctgctatcccgttcaacactctggctgaccattacggtgagcgtttagctcgcgaacagttggccctt
gagcatgagtcttacgagatgggtgaagcacgcttccgcaagatgtttgagcgtcaacttaaagctggtgaggttgcgg
ataacgctgccgccaagcctctcatcactaccctactccctaagatgattgcacgcatcaacgactggtttgaggaagt
gaaagctaagcgcggcaagcgcccgacagccttccagttcctgcaagaaatcaagccggaagccgtagcgtacatcacc
attaagaccactctggcttgcctaaccagtgctgacaatacaaccgttcaggctgtagcaagcgcaatcggtcgggcca
ttgaggacgaggctcgcttcggtcgtatccgtgaccttgaagctaagcacttcaagaaaaacgttgaggaacaactcaa
caagcgcgtagggcacgtctacaagaaagcatttatgcaagttgtcgaggctgacatgctctctaagggtctactcggt
ggcgaggcgtggtcttcgtggcataaggaagactctattcatgtaggagtacgctgcatcgagatgctcattgagtcaa
ccggaatggttagcttacaccgccaaaatgctggcgtagtaggtcaagactctgagactatcgaactcgcacctgaata
cgctgaggctatcgcaacccgtgcaggtgcgctggctggcatctctccgatgttccaaccttgcgtagttcctcctaag
ccgtggactggcattactggtggtggctattgggctaacggtcgtcgtcctctggcgctggtgcgtactcacagtaaga
aagcactgatgcgctacgaagacgtttacatgcctgaggtgtacaaagcgattaacattgcgcaaaacaccgcatggaa
aatcaacaagaaagtcctagcggtcgccaacgtaatcaccaagtggaagcattgtccggtcgaggacatccctgcgatt
gagcgtgaagaactcccgatgaaaccggaagacatcgacatgaatcctgaggctctcaccgcgtggaaacgtgctgccg
ctgctgtgtaccgcaaggacaaggctcgcaagtctcgccgtatcagccttgagttcatgcttgagcaagccaataagtt
tgctaaccataaggccatctggttcccttacaacatggactggcgcggtcgtgtttacgctgtgtcaatgttcaacccg
caaggtaacgatatgaccaaaggactgcttacgctggcgaaaggtaaaccaatcggtaaggaaggttactactggctga
aaatccacggtgcaaactgtgcgggtgtcgataaggttccgttccctgagcgcatcaagttcattgaggaaaaccacga
gaacatcatggcttgcgctaagtctccactggagaacacttggtgggctgagcaagattctccgttctgcttccttgcg
ttctgctttgagtacgctggggtacagcaccacggcctgagctataactgctcccttccgctggcgtttgacgggtctt
gctctggcatccagcacttctccgcgatgctccgagatgaggtaggtggtcgcgcggttaacttgcttcctagtgaaac
cgttcaggacatctacgggattgttgctaagaaagtcaacgagattctacaagcagacgcaatcaatgggaccgataac
gaagtagttaccgtgaccgatgagaacactggtgaaatctctgagaaagtcaagctgggcactaaggcactggctggtc
aatggctggcttacggtgttactcgcagtgtgactaagcgttcagtcatgacgctggcttacgggtccaaagagttcgg
cttccgtcaacaagtgctggaagataccattcagccagctattgattccggcaagggtctgatgttcactcagccgaat
caggctgctggatacatggctaagctgatttgggaatctgtgagcgtgacggtggtagctgcggttgaagcaatgaact
ggcttaagtctgctgctaagctgctggctgctgaggtcaaagataagaagactggagagattcttcgcaagcgttgcgc
tgtgcattgggtaactcctgatggtttccctgtgtggcaggaatacaagaagcctattcagacgcgcttgaacctgatg
ttcctcggtcagttccgcttacagcctaccattaacaccaacaaagatagcgagattgatgcacacaaacaggagtctg
gtatcgctcctaactttgtacacagccaagacggtagccaccttcgtaagactgtagtgtgggcacacgagaagtacgg
aatcgaatcttttgcactgattcacgactccttcggtaccattccggctgacgctgcgaacctgttcaaagcagtgcgc
gaaactatggttgacacatatgagtcttgtgatgtactggctgatttctacgaccagttcgctgaccagttgcacgagt
ctcaattggacaaaatgccagcacttccggctaaaggtaacttgaacctccgtgacatcttagagtcggacttcgcgtt
cgcgtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttg
tttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata