BBa_K145217
type: ComponentDefinition
persistent identity: BBa K145217
display id: BBa_K145217
version: 1
was generated by: iGEM to SBOL conversion
description: FILTER Complete
created: 2008-08-19T11:00:00Z
modified: 2015-05-08T01:10:29Z
mutable provenance: Other parts + synthetic
owned by: james
owned by: myers
mutable description: FILTER This device was designed to filter out noise in an input signal; in our case OmpF PoPs. If there is a short increase in transcription from this promoter, a small amount of J23066 RiboKey is produced. This is then used to unlock (J23078) the short lived UmuD-tagged T7 RNA polymerase. This results in an increase of active T7 protein. The final part of this filter is an AND gate which is used in the components further on in the system. This AND gate consists of a T7 RNA polymerase promoter with another RiboLock (J23078 again). The AND gate will only function if the duration of the noise is sufficiently long and intense (= a signal) in order to have both the short-lived RiboKey AND T7 polymerase available at the same time and thus to produce the component.
discontinued: false
dominant: false
experience: experience/None
group u list: _257_
m user id: 0
owner id: 2970
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable notes: We did a lot of modeling on this device. The most efficient Berkeley 2006 key and lock have been used. The RiboKy might be required in higher copy number although modeling doesn't predict this.
star: false
creator: Jonas Demeulemeester
type: DnaRegion
role: SO:0000804
role: partType/Device
component: BBa_J23009
component: BBa_J23008
component: BBa_B0010
component: BBa_K145014
component: BBa_J23078
component: BBa_J23109
component: BBa_B0012
component: BBa_R0040
sequence annotation: BBa_R0040
sequence annotation: BBa_J23008
sequence annotation: BBa_J23009
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_J23109
sequence annotation: BBa_J23078
sequence annotation: BBa_K145014
sequence: https://synbiohub.org/public/igem/BBa_K145217_sequence/1

BBa_J23009
type: Component
persistent identity: component1974123
display id: component1974123
version: 1
top level: BBa_K145217
definition: BBa_J23009
access: public

BBa_J23008
type: Component
persistent identity: component1974122
display id: component1974122
version: 1
top level: BBa_K145217
definition: BBa_J23008
access: public

BBa_B0010
type: Component
persistent identity: component1974138
display id: component1974138
version: 1
top level: BBa_K145217
definition: BBa_B0010
access: public

BBa_K145014
type: Component
persistent identity: component1974137
display id: component1974137
version: 1
top level: BBa_K145217
definition: T7
access: public

BBa_J23078
type: Component
persistent identity: component1974131
display id: component1974131
version: 1
top level: BBa_K145217
definition: BBa_J23078
access: public

BBa_J23109
type: Component
persistent identity: component1974130
display id: component1974130
version: 1
top level: BBa_K145217
definition: BBa_J23109
access: public

BBa_B0012
type: Component
persistent identity: component1974140
display id: component1974140
version: 1
top level: BBa_K145217
definition: BBa_B0012
access: public

BBa_R0040
type: Component
persistent identity: component1974118
display id: component1974118
version: 1
top level: BBa_K145217
definition: p(tetR)
access: public

BBa_B0012
type: Component
persistent identity: component1974126
display id: component1974126
version: 1
top level: BBa_K145217
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1974124
display id: component1974124
version: 1
top level: BBa_K145217
definition: BBa_B0010
access: public

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1974118
display id: annotation1974118
version: 1
top level: BBa_K145217
location: https://synbiohub.org/public/igem/BBa_K145217/annotation1974118/range1974118/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K145217/annotation1974118/range1974118/1
type: Range
persistent identity: range1974118
display id: range1974118
version: 1
top level: BBa_K145217
direction: direction/forward
start: 1
end: 54
orientation: inline

BBa_J23008
type: SequenceAnnotation
persistent identity: annotation1974122
display id: annotation1974122
version: 1
top level: BBa_K145217
location: https://synbiohub.org/public/igem/BBa_K145217/annotation1974122/range1974122/1
component: BBa_J23008

https://synbiohub.org/public/igem/BBa_K145217/annotation1974122/range1974122/1
type: Range
persistent identity: range1974122
display id: range1974122
version: 1
top level: BBa_K145217
direction: direction/reverse
start: 63
end: 156
orientation: inline

BBa_J23009
type: SequenceAnnotation
persistent identity: annotation1974123
display id: annotation1974123
version: 1
top level: BBa_K145217
location: https://synbiohub.org/public/igem/BBa_K145217/annotation1974123/range1974123/1
component: BBa_J23009

https://synbiohub.org/public/igem/BBa_K145217/annotation1974123/range1974123/1
type: Range
persistent identity: range1974123
display id: range1974123
version: 1
top level: BBa_K145217
direction: direction/reverse
start: 165
end: 261
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1974124
display id: annotation1974124
version: 1
top level: BBa_K145217
location: https://synbiohub.org/public/igem/BBa_K145217/annotation1974124/range1974124/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K145217/annotation1974124/range1974124/1
type: Range
persistent identity: range1974124
display id: range1974124
version: 1
top level: BBa_K145217
direction: direction/forward
start: 270
end: 349
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1974126
display id: annotation1974126
version: 1
top level: BBa_K145217
location: https://synbiohub.org/public/igem/BBa_K145217/annotation1974126/range1974126/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K145217/annotation1974126/range1974126/1
type: Range
persistent identity: range1974126
display id: range1974126
version: 1
top level: BBa_K145217
direction: direction/forward
start: 358
end: 398
orientation: inline

BBa_J23109
type: SequenceAnnotation
persistent identity: annotation1974130
display id: annotation1974130
version: 1
top level: BBa_K145217
location: https://synbiohub.org/public/igem/BBa_K145217/annotation1974130/range1974130/1
component: BBa_J23109

https://synbiohub.org/public/igem/BBa_K145217/annotation1974130/range1974130/1
type: Range
persistent identity: range1974130
display id: range1974130
version: 1
top level: BBa_K145217
direction: direction/reverse
start: 407
end: 441
orientation: inline

BBa_J23078
type: SequenceAnnotation
persistent identity: annotation1974131
display id: annotation1974131
version: 1
top level: BBa_K145217
location: https://synbiohub.org/public/igem/BBa_K145217/annotation1974131/range1974131/1
component: BBa_J23078

https://synbiohub.org/public/igem/BBa_K145217/annotation1974131/range1974131/1
type: Range
persistent identity: range1974131
display id: range1974131
version: 1
top level: BBa_K145217
direction: direction/reverse
start: 450
end: 501
orientation: inline

BBa_K145014
type: SequenceAnnotation
persistent identity: annotation1974137
display id: annotation1974137
version: 1
top level: BBa_K145217
location: https://synbiohub.org/public/igem/BBa_K145217/annotation1974137/range1974137/1
component: BBa_K145014

https://synbiohub.org/public/igem/BBa_K145217/annotation1974137/range1974137/1
type: Range
persistent identity: range1974137
display id: range1974137
version: 1
top level: BBa_K145217
direction: direction/reverse
start: 508
end: 3249
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1974138
display id: annotation1974138
version: 1
top level: BBa_K145217
location: https://synbiohub.org/public/igem/BBa_K145217/annotation1974138/range1974138/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K145217/annotation1974138/range1974138/1
type: Range
persistent identity: range1974138
display id: range1974138
version: 1
top level: BBa_K145217
direction: direction/forward
start: 3258
end: 3337
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1974140
display id: annotation1974140
version: 1
top level: BBa_K145217
location: https://synbiohub.org/public/igem/BBa_K145217/annotation1974140/range1974140/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K145217/annotation1974140/range1974140/1
type: Range
persistent identity: range1974140
display id: range1974140
version: 1
top level: BBa_K145217
direction: direction/forward
start: 3346
end: 3386
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

p(tetR)
type: ComponentDefinition
persistent identity: BBa R0040
display id: BBa_R0040
version: 1
was generated by: iGEM to SBOL conversion
description: TetR repressible promoter
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: Lutz, R., Bujard, H., Nucleic Acids Research (1997) 25, 1203-1210.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Sequence for pTet inverting regulator driven by the TetR protein.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0040
sequence annotation: TetR 1
sequence annotation: -35
sequence annotation: TetR 2
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0040_sequence/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1986784
display id: annotation1986784
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
type: Range
persistent identity: range1986784
display id: range1986784
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 54
orientation: inline

TetR 1
type: SequenceAnnotation
persistent identity: annotation1986783
display id: annotation1986783
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
type: Range
persistent identity: range1986783
display id: range1986783
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1986785
display id: annotation1986785
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
type: Range
persistent identity: range1986785
display id: range1986785
version: 1
top level: p(tetR)
direction: direction/forward
start: 20
end: 25
orientation: inline

TetR 2
type: SequenceAnnotation
persistent identity: annotation1986786
display id: annotation1986786
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
type: Range
persistent identity: range1986786
display id: range1986786
version: 1
top level: p(tetR)
direction: direction/forward
start: 26
end: 44
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1986787
display id: annotation1986787
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
type: Range
persistent identity: range1986787
display id: range1986787
version: 1
top level: p(tetR)
direction: direction/forward
start: 43
end: 48
orientation: inline

BBa_J23008
type: ComponentDefinition
persistent identity: BBa J23008
display id: BBa_J23008
version: 1
was generated by: iGEM to SBOL conversion
description: [key3c] riboregulator for lock3 variants
created: 2006-07-09T11:00:00Z
modified: 2015-08-31T04:08:38Z
mutable provenance: J23007
owned by: james
owned by: myers
mutable description: This part has the same homology region to J01122 as J23007 except that unlike J23007, J23008 does not contain the hairpin that was derived from J01129. J23008 anneals linearly to the stem of the hairpin in J01122 with perfect base pairing and destroys the hairpin in the process and reveals the RBS on J01122. When J01122 is transcribed the RNA secondary structure is such that the RBS hairpins with sequence upstream preventing the ribosome from binding and translation from occuring thereby locking up RFP further down stream. When J23008 is introduced into the cell in trans, we suspect that it will unlock the RBS for RFP as described above allowing translation to occur.
discontinued: false
dominant: false
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 931
owning group id: 52
sample status: In stock
status: status/Available
bookmark: false
mutable notes: No design considerations
star: true
creator: Kaitlin Davis
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence: https://synbiohub.org/public/igem/BBa_J23008_sequence/1

BBa_J23009
type: ComponentDefinition
persistent identity: BBa J23009
display id: BBa_J23009
version: 1
was generated by: iGEM to SBOL conversion
description: [key3d] riboregulator for lock3 variants
created: 2006-08-02T11:00:00Z
modified: 2015-08-31T04:08:38Z
mutable provenance: Overlap extension
owned by: james
owned by: myers
mutable description: Variant of key3 (see part J01129 (key3), J23007(key3b), J23008(key3c)). Part is in pJ23006, so there is a Ptet upstream of the XbaI site to drive expression. Nevertheless, J23009 is still a basic part allowing both prefix and suffix insertions.
discontinued: false
dominant: false
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence: https://synbiohub.org/public/igem/BBa_J23009_sequence/1

BBa_J23078
type: ComponentDefinition
persistent identity: BBa J23078
display id: BBa_J23078
version: 1
was generated by: iGEM to SBOL conversion
description: [lock3i]
created: 2006-09-12T11:00:00Z
modified: 2015-08-31T04:08:39Z
mutable provenance: PCR ca1028F/G00101 on pJ23006-J23032 . . . (162 bp, XbaI/PstI) Sub into pSB1A2-I13521 . . . . . . . . . . (XbaI/PstI) Product is pSB1A2-J23078
owned by: james
owned by: myers
mutable description: lock3d derivative with a 5' extension. This is the basic part, J23077 is the RFP reporter.
discontinued: false
dominant: true
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: N/A
star: false
creator: John Anderson
type: DnaRegion
role: partType/RNA
role: SO:0000834
sequence: https://synbiohub.org/public/igem/BBa_J23078_sequence/1

BBa_J23109
type: ComponentDefinition
persistent identity: BBa J23109
display id: BBa_J23109
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive promoter family member
created: 2006-08-16T11:00:00Z
modified: 2015-08-31T04:08:40Z
mutable provenance: Later
owned by: james
owned by: myers
mutable description: Later
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23109_sequence/1

T7
type: ComponentDefinition
persistent identity: BBa K145014
display id: BBa_K145014
version: 1
was generated by: iGEM to SBOL conversion
description: T7 RNA Polymerase with UmuD derived tag
created: 2008-07-23T11:00:00Z
modified: 2015-05-08T01:10:28Z
mutable provenance: Part composed of the T7 RNA Polymerase and the N-terminal degradation tag, but with no scar (no stop codon)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: T7 RNA Polymerase with N-terminal UmuD degradation tag.
discontinued: false
dominant: false
experience: experience/None
group u list: _257_
m user id: 0
owner id: 2939
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: This part is different from the simple composition of the tag and the polymerase, as there cannot be a stop codon in the scar.
star: true
creator: Benjamien Moeyaert
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: tag
sequence annotation: start
sequence annotation: cds
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_K145014_sequence/1

tag
type: SequenceAnnotation
persistent identity: annotation1968241
display id: annotation1968241
version: 1
top level: T7
location: https://synbiohub.org/public/igem/BBa_K145014/annotation1968241/range1968241/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_K145014/annotation1968241/range1968241/1
type: Range
persistent identity: range1968241
display id: range1968241
version: 1
top level: T7
direction: direction/forward
start: 1
end: 87
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1968238
display id: annotation1968238
version: 1
top level: T7
location: https://synbiohub.org/public/igem/BBa_K145014/annotation1968238/range1968238/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_K145014/annotation1968238/range1968238/1
type: Range
persistent identity: range1968238
display id: range1968238
version: 1
top level: T7
direction: direction/forward
start: 1
end: 3
orientation: inline

cds
type: SequenceAnnotation
persistent identity: annotation1968242
display id: annotation1968242
version: 1
top level: T7
location: https://synbiohub.org/public/igem/BBa_K145014/annotation1968242/range1968242/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K145014/annotation1968242/range1968242/1
type: Range
persistent identity: range1968242
display id: range1968242
version: 1
top level: T7
direction: direction/forward
start: 88
end: 2736
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1968240
display id: annotation1968240
version: 1
top level: T7
location: https://synbiohub.org/public/igem/BBa_K145014/annotation1968240/range1968240/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_K145014/annotation1968240/range1968240/1
type: Range
persistent identity: range1968240
display id: range1968240
version: 1
top level: T7
direction: direction/forward
start: 2737
end: 2739
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1968239
display id: annotation1968239
version: 1
top level: T7
location: https://synbiohub.org/public/igem/BBa_K145014/annotation1968239/range1968239/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_K145014/annotation1968239/range1968239/1
type: Range
persistent identity: range1968239
display id: range1968239
version: 1
top level: T7
direction: direction/forward
start: 2740
end: 2742
orientation: inline

https://synbiohub.org/public/igem/BBa_K145217_sequence/1
type: Sequence
persistent identity: BBa K145217 sequence
display id: BBa_K145217_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagacccaaaagcaagaggtgattctagttggtggttaatgaaaattaacttacttactagaaatatctctaaaaagccagattattaatccggctttactagagcgggcgggcgggcgggcgggatccataaaaaaaaaacccaaaagcaagaggtgattctagttaaaaaaaaaaagcttcccgcccgcccgcccgcccgtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtttacagctagctcagtcctagggactgtgctagctactagagtgtagaccgaactagaatcacctcttgcttttgggtaagacagaagaggagatactagatgttgtttatcaagcctgcggatctccgcgaaattgtgacttttccgctatttagcgatcttgttcagtgtggctttccttcaccgatgaacacgattaacatcgctaagaacgacttctctgacatcgaactggctgctatcccgttcaacactctggctgaccattacggtgagcgtttagctcgcgaacagttggcccttgagcatgagtcttacgagatgggtgaagcacgcttccgcaagatgtttgagcgtcaacttaaagctggtgaggttgcggataacgctgccgccaagcctctcatcactaccctactccctaagatgattgcacgcatcaacgactggtttgaggaagtgaaagctaagcgcggcaagcgcccgacagccttccagttcctgcaagaaatcaagccggaagccgtagcgtacatcaccattaagaccactctggcttgcctaaccagtgctgacaatacaaccgttcaggctgtagcaagcgcaatcggtcgggccattgaggacgaggctcgcttcggtcgtatccgtgaccttgaagctaagcacttcaagaaaaacgttgaggaacaactcaacaagcgcgtagggcacgtctacaagaaagcatttatgcaagttgtcgaggctgacatgctctctaagggtctactcggtggcgaggcgtggtcttcgtggcataaggaagactctattcatgtaggagtacgctgcatcgagatgctcattgagtcaaccggaatggttagcttacaccgccaaaatgctggcgtagtaggtcaagactctgagactatcgaactcgcacctgaatacgctgaggctatcgcaacccgtgcaggtgcgctggctggcatctctccgatgttccaaccttgcgtagttcctcctaagccgtggactggcattactggtggtggctattgggctaacggtcgtcgtcctctggcgctggtgcgtactcacagtaagaaagcactgatgcgctacgaagacgtttacatgcctgaggtgtacaaagcgattaacattgcgcaaaacaccgcatggaaaatcaacaagaaagtcctagcggtcgccaacgtaatcaccaagtggaagcattgtccggtcgaggacatccctgcgattgagcgtgaagaactcccgatgaaaccggaagacatcgacatgaatcctgaggctctcaccgcgtggaaacgtgctgccgctgctgtgtaccgcaaggacaaggctcgcaagtctcgccgtatcagccttgagttcatgcttgagcaagccaataagtttgctaaccataaggccatctggttcccttacaacatggactggcgcggtcgtgtttacgctgtgtcaatgttcaacccgcaaggtaacgatatgaccaaaggactgcttacgctggcgaaaggtaaaccaatcggtaaggaaggttactactggctgaaaatccacggtgcaaactgtgcggg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0040_sequence/1
type: Sequence
persistent identity: BBa R0040 sequence
display id: BBa_R0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23008_sequence/1
type: Sequence
persistent identity: BBa J23008 sequence
display id: BBa_J23008_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acccaaaagcaagaggtgattctagttggtggttaatgaaaattaacttacttactagaaatatctctaaaaagccagattattaatccggctt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23009_sequence/1
type: Sequence
persistent identity: BBa J23009 sequence
display id: BBa_J23009_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cgggcgggcgggcgggcgggatccataaaaaaaaaacccaaaagcaagaggtgattctagttaaaaaaaaaaagcttcccgcccgcccgcccgcccg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23078_sequence/1
type: Sequence
persistent identity: BBa J23078 sequence
display id: BBa_J23078_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tgtagaccgaactagaatcacctcttgcttttgggtaagacagaagaggaga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23109_sequence/1
type: Sequence
persistent identity: BBa J23109 sequence
display id: BBa_J23109_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttacagctagctcagtcctagggactgtgctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K145014_sequence/1
type: Sequence
persistent identity: BBa K145014 sequence
display id: BBa_K145014_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgttgtttatcaagcctgcggatctccgcgaaattgtgacttttccgctatttagcgatcttgttcagtgtggctttccttcaccgatgaacacgattaacatcgctaagaacgacttctctgacatcgaactggctgctatcccgttcaacactctggctgaccattacggtgagcgtttagctcgcgaacagttggcccttgagcatgagtcttacgagatgggtgaagcacgcttccgcaagatgtttgagcgtcaacttaaagctggtgaggttgcggataacgctgccgccaagcctctcatcactaccctactccctaagatgattgcacgcatcaacgactggtttgaggaagtgaaagctaagcgcggcaagcgcccgacagccttccagttcctgcaagaaatcaagccggaagccgtagcgtacatcaccattaagaccactctggcttgcctaaccagtgctgacaatacaaccgttcaggctgtagcaagcgcaatcggtcgggccattgaggacgaggctcgcttcggtcgtatccgtgaccttgaagctaagcacttcaagaaaaacgttgaggaacaactcaacaagcgcgtagggcacgtctacaagaaagcatttatgcaagttgtcgaggctgacatgctctctaagggtctactcggtggcgaggcgtggtcttcgtggcataaggaagactctattcatgtaggagtacgctgcatcgagatgctcattgagtcaaccggaatggttagcttacaccgccaaaatgctggcgtagtaggtcaagactctgagactatcgaactcgcacctgaatacgctgaggctatcgcaacccgtgcaggtgcgctggctggcatctctccgatgttccaaccttgcgtagttcctcctaagccgtggactggcattactggtggtggctattgggctaacggtcgtcgtcctctggcgctggtgcgtactcacagtaagaaagcactgatgcgctacgaagacgtttacatgcctgaggtgtacaaagcgattaacattgcgcaaaacaccgcatggaaaatcaacaagaaagtcctagcggtcgccaacgtaatcaccaagtggaagcattgtccggtcgaggacatccctgcgattgagcgtgaagaactcccgatgaaaccggaagacatcgacatgaatcctgaggctctcaccgcgtggaaacgtgctgccgctgctgtgtaccgcaaggacaaggctcgcaagtctcgccgtatcagccttgagttcatgcttgagcaagccaataagtttgctaaccataaggccatctggttcccttacaacatggactggcgcggtcgtgtttacgctgtgtcaatgttcaacccgcaaggtaacgatatgaccaaaggactgcttacgctggcgaaaggtaaaccaatcggtaaggaaggttactactggctgaaaatccacggtgcaaactgtgcgggtgtcgataaggttccgttccctgagcgcatcaagttcattgaggaaaaccacgagaacatcatggcttgcgctaagtctccactggagaacacttggtgggctgagcaagattctccgttctgcttccttgcgttctgctttgagtacgctggggtacagcaccacggcctgagctataactgctcccttccgctggcgtttgacgggtcttgctctggcatccagcacttctccgcgatgctccgagatgaggtaggtggtcgcgcggttaacttgcttcctagtgaaaccgttcaggacatctacgggattgttgctaagaaagtcaacgagattctacaagcagacgcaatcaatgggaccgataacgaagtagttaccgtgaccgatgagaacactggtgaaatctctgagaaagtcaagctgggcactaaggcactggctggtcaatggctggcttacggtgttactcgcagtgtgactaagcgttcagtcatgacgctggc…
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z