LOCUS       BBa_K145220             2272 bp    DNA     linear       25-Nov-2024
DEFINITION  INVERTED TIMER
ACCESSION   BBa_K145220
VERSION     BBa_K145220.1  
FEATURES             Location/Qualifiers
     promoter        1..46
                     /label=BBa_I712074
     misc_feature    55..106
                     /label=BBa_J23078
     misc_feature    1202..1240
                     /label=LVA
     CDS             113..1240
                     /label=lacI-LVA
     misc_feature    113..1240
                     /label=BBa_C0012
     misc_feature    1241..1265
                     /label=Help:Barcodes
     CDS             113..1240
                     /label=BBa_C0012
     stem_loop       1285..1328
                     /label=stem_loop
     misc_feature    1274..1353
                     /label=BBa_B0010
     terminator      1274..1353
                     /label=BBa_B0010
     stem_loop       1369..1388
                     /label=T7 TE
     misc_feature    1395..1395
                     /label=stop
     polyA_site      1389..1402
                     /label=polya
     misc_feature    1362..1402
                     /label=BBa_B0012
     terminator      1362..1402
                     /label=BBa_B0012
     terminator      1274..1402
                     /label=BBa_B0015
     misc_feature    1413..1429
                     /label=lac O1
     promoter        1430..1435
                     /label=-35
     misc_feature    1436..1452
                     /label=lac O1
     promoter        1453..1458
                     /label=-10
     misc_feature    1411..1464
                     /label=BBa_R0011
     promoter        1411..1464
                     /label=BBa_R0011
     RBS             1474..1486
                     /label=RBS-3\Weak
     RBS             1480..1483
                     /label=RBS
     misc_feature    1474..1486
                     /label=BBa_B0032
     RBS             1474..1486
                     /label=BBa_B0032
     misc_feature    2072..2103
                     /label=LVA
     CDS             1493..2071
                     /label=luxI
     misc_feature    1493..2110
                     /label=BBa_C0061
     misc_feature    2111..2135
                     /label=Help:Barcodes
     CDS             1493..2110
                     /label=BBa_C0061
     stem_loop       2155..2198
                     /label=stem_loop
     misc_feature    2144..2223
                     /label=BBa_B0010
     terminator      2144..2223
                     /label=BBa_B0010
     stem_loop       2239..2258
                     /label=T7 TE
     misc_feature    2265..2265
                     /label=stop
     polyA_site      2259..2272
                     /label=polya
     misc_feature    2232..2272
                     /label=BBa_B0012
     terminator      2232..2272
                     /label=BBa_B0012
     terminator      2144..2272
                     /label=BBa_B0015
ORIGIN
        1 taatacgact cactataggg aatacaagct acttgttctt tttgcatact agagtgtaga
       61 ccgaactaga atcacctctt gcttttgggt aagacagaag aggagatact agatggtgaa
      121 tgtgaaacca gtaacgttat acgatgtcgc agagtatgcc ggtgtctctt atcagaccgt
      181 ttcccgcgtg gtgaaccagg ccagccacgt ttctgcgaaa acgcgggaaa aagtggaagc
      241 ggcgatggcg gagctgaatt acattcccaa ccgcgtggca caacaactgg cgggcaaaca
      301 gtcgttgctg attggcgttg ccacctccag tctggccctg cacgcgccgt cgcaaattgt
      361 cgcggcgatt aaatctcgcg ccgatcaact gggtgccagc gtggtggtgt cgatggtaga
      421 acgaagcggc gtcgaagcct gtaaagcggc ggtgcacaat cttctcgcgc aacgcgtcag
      481 tgggctgatc attaactatc cgctggatga ccaggatgcc attgctgtgg aagctgcctg
      541 cactaatgtt ccggcgttat ttcttgatgt ctctgaccag acacccatca acagtattat
      601 tttctcccat gaagacggta cgcgactggg cgtggagcat ctggtcgcat tgggtcacca
      661 gcaaatcgcg ctgttagcgg gcccattaag ttctgtctcg gcgcgtctgc gtctggctgg
      721 ctggcataaa tatctcactc gcaatcaaat tcagccgata gcggaacggg aaggcgactg
      781 gagtgccatg tccggttttc aacaaaccat gcaaatgctg aatgagggca tcgttcccac
      841 tgcgatgctg gttgccaacg atcagatggc gctgggcgca atgcgcgcca ttaccgagtc
      901 cgggctgcgc gttggtgcgg atatctcggt agtgggatac gacgataccg aagacagctc
      961 atgttatatc ccgccgttaa ccaccatcaa acaggatttt cgcctgctgg ggcaaaccag
     1021 cgtggaccgc ttgctgcaac tctctcaggg ccaggcggtg aagggcaatc agctgttgcc
     1081 cgtctcactg gtgaaaagaa aaaccaccct ggcgcccaat acgcaaaccg cctctccccg
     1141 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
     1201 ggctgcaaac gacgaaaact acgctttagt agcttaataa ctctgatagt gctagtgtag
     1261 atctctacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt
     1321 cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg
     1381 ggtgggcctt tctgcgttta tatactagag aattgtgagc ggataacaat tgacattgtg
     1441 agcggataac aagatactga gcacatacta gagtcacaca ggaaagtact agatgactat
     1501 aatgataaaa aaatcggatt ttttggcaat tccatcggag gagtataaag gtattctaag
     1561 tcttcgttat caagtgttta agcaaagact tgagtgggac ttagttgtag aaaataacct
     1621 tgaatcagat gagtatgata actcaaatgc agaatatatt tatgcttgtg atgatactga
     1681 aaatgtaagt ggatgctggc gtttattacc tacaacaggt gattatatgc tgaaaagtgt
     1741 ttttcctgaa ttgcttggtc aacagagtgc tcccaaagat cctaatatag tcgaattaag
     1801 tcgttttgct gtaggtaaaa atagctcaaa gataaataac tctgctagtg aaattacaat
     1861 gaaactattt gaagctatat ataaacacgc tgttagtcaa ggtattacag aatatgtaac
     1921 agtaacatca acagcaatag agcgattttt aaagcgtatt aaagttcctt gtcatcgtat
     1981 tggagacaaa gaaattcatg tattaggtga tactaaatcg gttgtattgt ctatgcctat
     2041 taatgaacag tttaaaaaag cagtcttaaa tgctgcaaac gacgaaaact acgctttagt
     2101 agcttaataa ctctgatagt gctagtgtag atctctacta gagccaggca tcaaataaaa
     2161 cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct
     2221 ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta ta
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