##gff-version 3
##sequence-region BBa_K145220 1 2272
BBa_K145220	.	CDS	113	1240	.	+	0	ID=BBa_C0012;Name=BBa_C0012
BBa_K145220	.	engineered_region	113	1240	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_K145220	.	engineered_tag	1241	1265	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_K145220	.	CDS	113	1240	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_K145220	.	tag	1202	1240	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_K145220	.	ribosome_entry_site	1474	1486	.	+	0	ID=BBa_B0032;Name=BBa_B0032
BBa_K145220	.	ribosome_entry_site	1474	1486	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K145220	.	engineered_region	1474	1486	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K145220	.	ribosome_entry_site	1480	1483	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K145220	.	terminator	1274	1402	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K145220	.	terminator	1274	1353	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K145220	.	stem_loop	1285	1328	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K145220	.	engineered_region	1274	1353	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K145220	.	terminator	1362	1402	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K145220	.	polyA_site	1389	1402	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K145220	.	stem_loop	1369	1388	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K145220	.	stop_codon	1395	1395	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K145220	.	engineered_region	1362	1402	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K145220	.	promoter	1	46	.	+	0	ID=BBa_I712074;Name=BBa_I712074
BBa_K145220	.	CDS	1493	2110	.	+	0	ID=BBa_C0061;Name=BBa_C0061
BBa_K145220	.	engineered_region	1493	2110	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_K145220	.	CDS	1493	2071	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_K145220	.	engineered_tag	2111	2135	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_K145220	.	tag	2072	2103	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_K145220	.	promoter	1411	1464	.	+	0	ID=BBa_R0011;Name=BBa_R0011
BBa_K145220	.	promoter	1453	1458	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_K145220	.	promoter	1430	1435	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_K145220	.	operator	1436	1452	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_K145220	.	operator	1413	1429	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_K145220	.	engineered_region	1411	1464	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_K145220	.	mature_transcript_region	55	106	.	+	0	ID=BBa_J23078;Name=BBa_J23078
BBa_K145220	.	terminator	2144	2272	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K145220	.	terminator	2144	2223	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K145220	.	stem_loop	2155	2198	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K145220	.	engineered_region	2144	2223	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K145220	.	terminator	2232	2272	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K145220	.	polyA_site	2259	2272	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K145220	.	stem_loop	2239	2258	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K145220	.	stop_codon	2265	2265	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K145220	.	engineered_region	2232	2272	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
>BBa_K145220
taatacgactcactatagggaatacaagctacttgttctttttgcatactagagtgtagaccgaactagaatcacctct
tgcttttgggtaagacagaagaggagatactagatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatg
ccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtgga
agcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggc
gttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtg
ccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacg
cgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccg
gcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcg
tggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcg
tctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatg
tccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatgg
cgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgatac
cgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggac
cgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaacca
ccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccg
actggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatct
ctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggt
gaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagaattgtgagcgg
ataacaattgacattgtgagcggataacaagatactgagcacatactagagtcacacaggaaagtactagatgactata
atgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgttta
agcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatat
ttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagt
gtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggta
aaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttag
tcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcat
cgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagttta
aaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatc
tctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcgg
tgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata