LOCUS       BBa_K145230             1444 bp    DNA     linear       17-Nov-2024
DEFINITION  CELL DEATH
ACCESSION   BBa_K145230
VERSION     BBa_K145230.1  
FEATURES             Location/Qualifiers
     misc_feature    1..20
                     /label=Lux-box
     promoter        20..25
                     /label=-35
     misc_feature    23..40
                     /label=OR2
     promoter        42..47
                     /label=-10
     misc_feature    46..63
                     /label=OR1
     promoter        1..66
                     /label=BBa_K145150
     misc_feature    79..82
                     /label=conserved
     RBS             75..86
                     /label=BBa_B0034
     misc_feature    93..94
                     /label=prefix
     misc_feature    266..266
                     /label=T
     misc_feature    584..584
                     /label=A
     CDS             93..842
                     /label=luxR
     misc_feature    93..848
                     /label=BBa_C0062
     misc_feature    849..873
                     /label=Help:Barcodes
     CDS             93..848
                     /label=BBa_C0062
     stem_loop       889..908
                     /label=T7 TE
     misc_feature    915..915
                     /label=stop
     polyA_site      909..922
                     /label=polya
     misc_feature    882..922
                     /label=BBa_B0012
     terminator      882..922
                     /label=BBa_B0012
     stem_loop       943..965
                     /label=stem_loop
     misc_feature    931..976
                     /label=BBa_B0011
     terminator      931..976
                     /label=BBa_B0011
     terminator      882..976
                     /label=BBa_B0014
     RBS             985..995
                     /label=RBS-4\Weaker
     RBS             991..994
                     /label=RBS
     misc_feature    985..995
                     /label=BBa_B0033
     RBS             985..995
                     /label=BBa_B0033
     misc_feature    1002..1004
                     /label=start
     misc_feature    1305..1307
                     /label=stop
     CDS             1002..1304
                     /label=cds
     CDS             1002..1307
                     /label=BBa_K145151
     stem_loop       1327..1370
                     /label=stem_loop
     misc_feature    1316..1395
                     /label=BBa_B0010
     terminator      1316..1395
                     /label=BBa_B0010
     stem_loop       1411..1430
                     /label=T7 TE
     misc_feature    1437..1437
                     /label=stop
     polyA_site      1431..1444
                     /label=polya
     misc_feature    1404..1444
                     /label=BBa_B0012
     terminator      1404..1444
                     /label=BBa_B0012
     terminator      1316..1444
                     /label=BBa_B0015
ORIGIN
        1 acctgtagga tcgtacaggt ttactaaaga ttcctttagt ttataattta agtgttcttt
       61 aatttctact agagaaagag gagaaatact agatgaaaaa cataaatgcc gacgacacat
      121 acagaataat taataaaatt aaagcttgta gaagcaataa tgatattaat caatgcttat
      181 ctgatatgac taaaatggta cattgtgaat attatttact cgcgatcatt tatcctcatt
      241 ctatggttaa atctgatatt tcaatcctag ataattaccc taaaaaatgg aggcaatatt
      301 atgatgacgc taatttaata aaatatgatc ctatagtaga ttattctaac tccaatcatt
      361 caccaattaa ttggaatata tttgaaaaca atgctgtaaa taaaaaatct ccaaatgtaa
      421 ttaaagaagc gaaaacatca ggtcttatca ctgggtttag tttccctatt catacggcta
      481 acaatggctt cggaatgctt agttttgcac attcagaaaa agacaactat atagatagtt
      541 tatttttaca tgcgtgtatg aacataccat taattgttcc ttctctagtt gataattatc
      601 gaaaaataaa tatagcaaat aataaatcaa acaacgattt aaccaaaaga gaaaaagaat
      661 gtttagcgtg ggcatgcgaa ggaaaaagct cttgggatat ttcaaaaata ttaggttgca
      721 gtgagcgtac tgtcactttc catttaacca atgcgcaaat gaaactcaat acaacaaacc
      781 gctgccaaag tatttctaaa gcaattttaa caggagcaat tgattgccca tactttaaaa
      841 attaataaca ctgatagtgc tagtgtagat cactactaga gtcacactgg ctcaccttcg
      901 ggtgggcctt tctgcgttta tatactagag agagaatata aaaagccaga ttattaatcc
      961 ggctttttta ttattttact agagtcacac aggactacta gatgcagttt aaggtttaca
     1021 cctataaaag agagagccgt tatcgtctgt ttgtggatgt acagagtgat attattgaca
     1081 cgcccgggcg acggatggtg atccccctgg ccagtgcacg tctgctgtca gataaagtct
     1141 cccgtgaact ttacccggtg gtgcatatcg gggatgaaag ctggcgcatg atgaccaccg
     1201 atatggccag tgtgccggtc tccgttatcg gggaagaagt ggctgatctc agccaccgcg
     1261 aaaatgacat caaaaacgcc attaacctga tgttctgggg aatataatac tagagccagg
     1321 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg
     1381 tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt
     1441 tata
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