##gff-version 3
##sequence-region BBa_K145230 1 1444
BBa_K145230	.	promoter	1	66	.	+	0	ID=BBa_K145150;Name=BBa_K145150
BBa_K145230	.	promoter	42	47	.	+	0	ID=annotation1971585;Name=-10;Parent=BBa_K145150
BBa_K145230	.	non_covalent_binding_site	1	20	.	+	0	ID=annotation1971582;Name=Lux-box;Parent=BBa_K145150
BBa_K145230	.	promoter	20	25	.	+	0	ID=annotation1971583;Name=-35;Parent=BBa_K145150
BBa_K145230	.	non_covalent_binding_site	23	40	.	+	0	ID=annotation1971584;Name=OR2;Parent=BBa_K145150
BBa_K145230	.	non_covalent_binding_site	46	63	.	+	0	ID=annotation1971586;Name=OR1;Parent=BBa_K145150
BBa_K145230	.	ribosome_entry_site	75	86	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K145230	.	conserved_region	79	82	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K145230	.	terminator	882	976	.	+	0	ID=BBa_B0014;Name=BBa_B0014
BBa_K145230	.	terminator	882	922	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0014
BBa_K145230	.	polyA_site	909	922	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K145230	.	stop_codon	915	915	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K145230	.	stem_loop	889	908	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K145230	.	engineered_region	882	922	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K145230	.	terminator	931	976	.	+	0	ID=BBa_B0011;Name=BBa_B0011;Parent=BBa_B0014
BBa_K145230	.	engineered_region	931	976	.	+	0	ID=annotation7019;Name=BBa_B0011;Parent=BBa_B0011
BBa_K145230	.	stem_loop	943	965	.	+	0	ID=annotation1683;Name=stem_loop;Parent=BBa_B0011
BBa_K145230	.	CDS	93	848	.	+	0	ID=BBa_C0062;Name=BBa_C0062
BBa_K145230	.	engineered_region	93	848	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K145230	.	engineered_tag	849	873	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K145230	.	engineered_region	93	94	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K145230	.	point_mutation	266	266	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K145230	.	point_mutation	584	584	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_K145230	.	CDS	93	842	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K145230	.	ribosome_entry_site	985	995	.	+	0	ID=BBa_B0033;Name=BBa_B0033
BBa_K145230	.	ribosome_entry_site	985	995	.	+	0	ID=annotation1713;Name=RBS-4\Weaker;Parent=BBa_B0033
BBa_K145230	.	engineered_region	985	995	.	+	0	ID=annotation7028;Name=BBa_B0033;Parent=BBa_B0033
BBa_K145230	.	ribosome_entry_site	991	994	.	+	0	ID=annotation1714;Name=RBS;Parent=BBa_B0033
BBa_K145230	.	CDS	1002	1307	.	+	0	ID=BBa_K145151;Name=BBa_K145151
BBa_K145230	.	CDS	1002	1304	.	+	0	ID=annotation1970253;Name=cds;Parent=BBa_K145151
BBa_K145230	.	stop_codon	1305	1307	.	+	0	ID=annotation1970252;Name=stop;Parent=BBa_K145151
BBa_K145230	.	start_codon	1002	1004	.	+	0	ID=annotation1970251;Name=start;Parent=BBa_K145151
BBa_K145230	.	terminator	1316	1444	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K145230	.	terminator	1404	1444	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K145230	.	polyA_site	1431	1444	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K145230	.	stop_codon	1437	1437	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K145230	.	stem_loop	1411	1430	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K145230	.	engineered_region	1404	1444	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K145230	.	terminator	1316	1395	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K145230	.	stem_loop	1327	1370	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K145230	.	engineered_region	1316	1395	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
>BBa_K145230
acctgtaggatcgtacaggtttactaaagattcctttagtttataatttaagtgttctttaatttctactagagaaaga
ggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaat
aatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctc
attctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaattt
aataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgct
gtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcata
cggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgc
gtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaac
aacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatat
taggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaag
tatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtaga
tcactactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagcca
gattattaatccggcttttttattattttactagagtcacacaggactactagatgcagtttaaggtttacacctataa
aagagagagccgttatcgtctgtttgtggatgtacagagtgatattattgacacgcccgggcgacggatggtgatcccc
ctggccagtgcacgtctgctgtcagataaagtctcccgtgaactttacccggtggtgcatatcggggatgaaagctggc
gcatgatgaccaccgatatggccagtgtgccggtctccgttatcggggaagaagtggctgatctcagccaccgcgaaaa
tgacatcaaaaacgccattaacctgatgttctggggaatataatactagagccaggcatcaaataaaacgaaaggctca
gtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcg
ggtgggcctttctgcgtttata