BBa_K145250
type: ComponentDefinition
persistent identity: BBa K145250
display id: BBa_K145250
version: 1
was generated by: iGEM to SBOL conversion
description: OLD FILTER T7 RNA pol
created: 2008-09-05T11:00:00Z
modified: 2015-05-08T01:10:29Z
mutable provenance: other parts
owned by: james
owned by: myers
mutable description: FILTER This device was designed to filter out noise in an input signal; in our case p(Tet) PoPs. If there is a short increase in transcription from this promoter, a small amount of J23009 RiboKey is produced. This is then used to unlock (J23032) the short lived UmuD-tagged T7 RNA polymerase. This results in an increase of active T7 protein. The final part of this filter is an AND gate which is used in the components further on in the system. This AND gate consists of a T7 RNA polymerase promoter with another RiboLock (J23032 again). The AND gate will only function if the duration of the noise is sufficiently long and intense (= a signal) in order to have both the short-lived RiboKey AND T7 polymerase available at the same time and thus to produce the component.
discontinued: true
dominant: false
experience: experience/None
group u list: _257_
m user id: 0
owner id: 2970
owning group id: 9
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable notes: Ribokey present in higher copy number? In the new system, the lock and key have been replaced by more efficient ones
star: false
is replaced by: BBa_K145103
creator: Jonas Demeulemeester
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_B0010
component: BBa_K145014
component: BBa_B0012
component: BBa_J23032
component: BBa_J23109
sequence annotation: BBa_J23109
sequence annotation: BBa_J23032
sequence annotation: BBa_K145014
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_K145250_sequence/1

BBa_B0010
type: Component
persistent identity: component1974794
display id: component1974794
version: 1
top level: BBa_K145250
definition: BBa_B0010
access: public

BBa_K145014
type: Component
persistent identity: component1974793
display id: component1974793
version: 1
top level: BBa_K145250
definition: T7
access: public

BBa_B0012
type: Component
persistent identity: component1974796
display id: component1974796
version: 1
top level: BBa_K145250
definition: BBa_B0012
access: public

BBa_J23032
type: Component
persistent identity: component1974787
display id: component1974787
version: 1
top level: BBa_K145250
definition: BBa_J23032
access: public

BBa_J23109
type: Component
persistent identity: component1974786
display id: component1974786
version: 1
top level: BBa_K145250
definition: BBa_J23109
access: public

BBa_J23109
type: SequenceAnnotation
persistent identity: annotation1974786
display id: annotation1974786
version: 1
top level: BBa_K145250
location: https://synbiohub.org/public/igem/BBa_K145250/annotation1974786/range1974786/1
component: BBa_J23109

https://synbiohub.org/public/igem/BBa_K145250/annotation1974786/range1974786/1
type: Range
persistent identity: range1974786
display id: range1974786
version: 1
top level: BBa_K145250
direction: direction/reverse
start: 1
end: 35
orientation: inline

BBa_J23032
type: SequenceAnnotation
persistent identity: annotation1974787
display id: annotation1974787
version: 1
top level: BBa_K145250
location: https://synbiohub.org/public/igem/BBa_K145250/annotation1974787/range1974787/1
component: BBa_J23032

https://synbiohub.org/public/igem/BBa_K145250/annotation1974787/range1974787/1
type: Range
persistent identity: range1974787
display id: range1974787
version: 1
top level: BBa_K145250
direction: direction/reverse
start: 44
end: 86
orientation: inline

BBa_K145014
type: SequenceAnnotation
persistent identity: annotation1974793
display id: annotation1974793
version: 1
top level: BBa_K145250
location: https://synbiohub.org/public/igem/BBa_K145250/annotation1974793/range1974793/1
component: BBa_K145014

https://synbiohub.org/public/igem/BBa_K145250/annotation1974793/range1974793/1
type: Range
persistent identity: range1974793
display id: range1974793
version: 1
top level: BBa_K145250
direction: direction/reverse
start: 93
end: 2834
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1974794
display id: annotation1974794
version: 1
top level: BBa_K145250
location: https://synbiohub.org/public/igem/BBa_K145250/annotation1974794/range1974794/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K145250/annotation1974794/range1974794/1
type: Range
persistent identity: range1974794
display id: range1974794
version: 1
top level: BBa_K145250
direction: direction/forward
start: 2843
end: 2922
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1974796
display id: annotation1974796
version: 1
top level: BBa_K145250
location: https://synbiohub.org/public/igem/BBa_K145250/annotation1974796/range1974796/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K145250/annotation1974796/range1974796/1
type: Range
persistent identity: range1974796
display id: range1974796
version: 1
top level: BBa_K145250
direction: direction/forward
start: 2931
end: 2971
orientation: inline

BBa_K145103
type: ComponentDefinition
persistent identity: BBa K145103
display id: BBa_K145103
version: 1
was generated by: iGEM to SBOL conversion
description: T7 with UmuD derived tag under constitutive promotor + RiboLock3d
created: 2008-07-09T11:00:00Z
modified: 2015-05-08T01:10:28Z
mutable provenance: other parts
owned by: james
owned by: myers
mutable description: T7 with an LVA tag under control of a constitutive promotor and a RiboLock3d
discontinued: false
dominant: true
group u list: _257_
m user id: 0
owner id: 2970
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: promotor weak enough? We took medium strength
star: false
creator: Jonas Demeulemeester
type: DnaRegion
role: partType/Other
role: SO:0000110
component: BBa_B0010
component: BBa_K145014
component: BBa_B0012
component: BBa_J23032
component: BBa_J23109
sequence annotation: BBa_J23109
sequence annotation: BBa_J23032
sequence annotation: BBa_K145014
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_K145103_sequence/1

BBa_B0010
type: Component
persistent identity: component1971380
display id: component1971380
version: 1
top level: BBa_K145103
definition: BBa_B0010
access: public

BBa_K145014
type: Component
persistent identity: component1971379
display id: component1971379
version: 1
top level: BBa_K145103
definition: T7
access: public

BBa_B0012
type: Component
persistent identity: component1971382
display id: component1971382
version: 1
top level: BBa_K145103
definition: BBa_B0012
access: public

BBa_J23032
type: Component
persistent identity: component1971373
display id: component1971373
version: 1
top level: BBa_K145103
definition: BBa_J23032
access: public

BBa_J23109
type: Component
persistent identity: component1971372
display id: component1971372
version: 1
top level: BBa_K145103
definition: BBa_J23109
access: public

BBa_J23109
type: SequenceAnnotation
persistent identity: annotation1971372
display id: annotation1971372
version: 1
top level: BBa_K145103
location: https://synbiohub.org/public/igem/BBa_K145103/annotation1971372/range1971372/1
component: BBa_J23109

https://synbiohub.org/public/igem/BBa_K145103/annotation1971372/range1971372/1
type: Range
persistent identity: range1971372
display id: range1971372
version: 1
top level: BBa_K145103
direction: direction/reverse
start: 1
end: 35
orientation: inline

BBa_J23032
type: SequenceAnnotation
persistent identity: annotation1971373
display id: annotation1971373
version: 1
top level: BBa_K145103
location: https://synbiohub.org/public/igem/BBa_K145103/annotation1971373/range1971373/1
component: BBa_J23032

https://synbiohub.org/public/igem/BBa_K145103/annotation1971373/range1971373/1
type: Range
persistent identity: range1971373
display id: range1971373
version: 1
top level: BBa_K145103
direction: direction/reverse
start: 44
end: 86
orientation: inline

BBa_K145014
type: SequenceAnnotation
persistent identity: annotation1971379
display id: annotation1971379
version: 1
top level: BBa_K145103
location: https://synbiohub.org/public/igem/BBa_K145103/annotation1971379/range1971379/1
component: BBa_K145014

https://synbiohub.org/public/igem/BBa_K145103/annotation1971379/range1971379/1
type: Range
persistent identity: range1971379
display id: range1971379
version: 1
top level: BBa_K145103
direction: direction/reverse
start: 93
end: 2834
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1971380
display id: annotation1971380
version: 1
top level: BBa_K145103
location: https://synbiohub.org/public/igem/BBa_K145103/annotation1971380/range1971380/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K145103/annotation1971380/range1971380/1
type: Range
persistent identity: range1971380
display id: range1971380
version: 1
top level: BBa_K145103
direction: direction/forward
start: 2843
end: 2922
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1971382
display id: annotation1971382
version: 1
top level: BBa_K145103
location: https://synbiohub.org/public/igem/BBa_K145103/annotation1971382/range1971382/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K145103/annotation1971382/range1971382/1
type: Range
persistent identity: range1971382
display id: range1971382
version: 1
top level: BBa_K145103
direction: direction/forward
start: 2931
end: 2971
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

BBa_J23032
type: ComponentDefinition
persistent identity: BBa J23032
display id: BBa_J23032
version: 1
was generated by: iGEM to SBOL conversion
description: [lock3d]
created: 2006-08-02T11:00:00Z
modified: 2015-08-31T04:08:39Z
mutable provenance: Extension of overlapping oligonucleotides
owned by: james
owned by: myers
mutable description: lock3d
discontinued: false
dominant: false
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: partType/RNA
role: SO:0000834
sequence: https://synbiohub.org/public/igem/BBa_J23032_sequence/1

BBa_J23109
type: ComponentDefinition
persistent identity: BBa J23109
display id: BBa_J23109
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive promoter family member
created: 2006-08-16T11:00:00Z
modified: 2015-08-31T04:08:40Z
mutable provenance: Later
owned by: james
owned by: myers
mutable description: Later
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23109_sequence/1

T7
type: ComponentDefinition
persistent identity: BBa K145014
display id: BBa_K145014
version: 1
was generated by: iGEM to SBOL conversion
description: T7 RNA Polymerase with UmuD derived tag
created: 2008-07-23T11:00:00Z
modified: 2015-05-08T01:10:28Z
mutable provenance: Part composed of the T7 RNA Polymerase and the N-terminal degradation tag, but with no scar (no stop codon)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: T7 RNA Polymerase with N-terminal UmuD degradation tag.
discontinued: false
dominant: false
experience: experience/None
group u list: _257_
m user id: 0
owner id: 2939
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: This part is different from the simple composition of the tag and the polymerase, as there cannot be a stop codon in the scar.
star: true
creator: Benjamien Moeyaert
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: tag
sequence annotation: start
sequence annotation: cds
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_K145014_sequence/1

tag
type: SequenceAnnotation
persistent identity: annotation1968241
display id: annotation1968241
version: 1
top level: T7
location: https://synbiohub.org/public/igem/BBa_K145014/annotation1968241/range1968241/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_K145014/annotation1968241/range1968241/1
type: Range
persistent identity: range1968241
display id: range1968241
version: 1
top level: T7
direction: direction/forward
start: 1
end: 87
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1968238
display id: annotation1968238
version: 1
top level: T7
location: https://synbiohub.org/public/igem/BBa_K145014/annotation1968238/range1968238/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_K145014/annotation1968238/range1968238/1
type: Range
persistent identity: range1968238
display id: range1968238
version: 1
top level: T7
direction: direction/forward
start: 1
end: 3
orientation: inline

cds
type: SequenceAnnotation
persistent identity: annotation1968242
display id: annotation1968242
version: 1
top level: T7
location: https://synbiohub.org/public/igem/BBa_K145014/annotation1968242/range1968242/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K145014/annotation1968242/range1968242/1
type: Range
persistent identity: range1968242
display id: range1968242
version: 1
top level: T7
direction: direction/forward
start: 88
end: 2736
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1968240
display id: annotation1968240
version: 1
top level: T7
location: https://synbiohub.org/public/igem/BBa_K145014/annotation1968240/range1968240/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_K145014/annotation1968240/range1968240/1
type: Range
persistent identity: range1968240
display id: range1968240
version: 1
top level: T7
direction: direction/forward
start: 2737
end: 2739
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1968239
display id: annotation1968239
version: 1
top level: T7
location: https://synbiohub.org/public/igem/BBa_K145014/annotation1968239/range1968239/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_K145014/annotation1968239/range1968239/1
type: Range
persistent identity: range1968239
display id: range1968239
version: 1
top level: T7
direction: direction/forward
start: 2740
end: 2742
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

https://synbiohub.org/public/igem/BBa_K145250_sequence/1
type: Sequence
persistent identity: BBa K145250 sequence
display id: BBa_K145250_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttacagctagctcagtcctagggactgtgctagctactagagaactagaatcacctcttgcttttgggtaagacagaagaggagatactagatgttgtttatcaagcctgcggatctccgcgaaattgtgacttttccgctatttagcgatcttgttcagtgtggctttccttcaccgatgaacacgattaacatcgctaagaacgacttctctgacatcgaactggctgctatcccgttcaacactctggctgaccattacggtgagcgtttagctcgcgaacagttggcccttgagcatgagtcttacgagatgggtgaagcacgcttccgcaagatgtttgagcgtcaacttaaagctggtgaggttgcggataacgctgccgccaagcctctcatcactaccctactccctaagatgattgcacgcatcaacgactggtttgaggaagtgaaagctaagcgcggcaagcgcccgacagccttccagttcctgcaagaaatcaagccggaagccgtagcgtacatcaccattaagaccactctggcttgcctaaccagtgctgacaatacaaccgttcaggctgtagcaagcgcaatcggtcgggccattgaggacgaggctcgcttcggtcgtatccgtgaccttgaagctaagcacttcaagaaaaacgttgaggaacaactcaacaagcgcgtagggcacgtctacaagaaagcatttatgcaagttgtcgaggctgacatgctctctaagggtctactcggtggcgaggcgtggtcttcgtggcataaggaagactctattcatgtaggagtacgctgcatcgagatgctcattgagtcaaccggaatggttagcttacaccgccaaaatgctggcgtagtaggtcaagactctgagactatcgaactcgcacctgaatacgctgaggctatcgcaacccgtgcaggtgcgctggctggcatctctccgatgttccaaccttgcgtagttcctcctaagccgtggactggcattactggtggtggctattgggctaacggtcgtcgtcctctggcgctggtgcgtactcacagtaagaaagcactgatgcgctacgaagacgtttacatgcctgaggtgtacaaagcgattaacattgcgcaaaacaccgcatggaaaatcaacaagaaagtcctagcggtcgccaacgtaatcaccaagtggaagcattgtccggtcgaggacatccctgcgattgagcgtgaagaactcccgatgaaaccggaagacatcgacatgaatcctgaggctctcaccgcgtggaaacgtgctgccgctgctgtgtaccgcaaggacaaggctcgcaagtctcgccgtatcagccttgagttcatgcttgagcaagccaataagtttgctaaccataaggccatctggttcccttacaacatggactggcgcggtcgtgtttacgctgtgtcaatgttcaacccgcaaggtaacgatatgaccaaaggactgcttacgctggcgaaaggtaaaccaatcggtaaggaaggttactactggctgaaaatccacggtgcaaactgtgcgggtgtcgataaggttccgttccctgagcgcatcaagttcattgaggaaaaccacgagaacatcatggcttgcgctaagtctccactggagaacacttggtgggctgagcaagattctccgttctgcttccttgcgttctgctttgagtacgctggggtacagcaccacggcctgagctataactgctcccttccgctggcgtttgacgggtcttgctctggcatccagcacttctccgcgatgctccgagatgaggtaggtggtcgcgcggttaacttgcttcctagtgaaaccgttcaggacatctacgggattgttgctaagaaagtcaacgagattctacaagcagacgcaatcaatgggaccgataacgaagtagttaccgtgaccgatgagaacactggtgaaatctctgag…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K145103_sequence/1
type: Sequence
persistent identity: BBa K145103 sequence
display id: BBa_K145103_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttacagctagctcagtcctagggactgtgctagctactagagaactagaatcacctcttgcttttgggtaagacagaagaggagatactagatgttgtttatcaagcctgcggatctccgcgaaattgtgacttttccgctatttagcgatcttgttcagtgtggctttccttcaccgatgaacacgattaacatcgctaagaacgacttctctgacatcgaactggctgctatcccgttcaacactctggctgaccattacggtgagcgtttagctcgcgaacagttggcccttgagcatgagtcttacgagatgggtgaagcacgcttccgcaagatgtttgagcgtcaacttaaagctggtgaggttgcggataacgctgccgccaagcctctcatcactaccctactccctaagatgattgcacgcatcaacgactggtttgaggaagtgaaagctaagcgcggcaagcgcccgacagccttccagttcctgcaagaaatcaagccggaagccgtagcgtacatcaccattaagaccactctggcttgcctaaccagtgctgacaatacaaccgttcaggctgtagcaagcgcaatcggtcgggccattgaggacgaggctcgcttcggtcgtatccgtgaccttgaagctaagcacttcaagaaaaacgttgaggaacaactcaacaagcgcgtagggcacgtctacaagaaagcatttatgcaagttgtcgaggctgacatgctctctaagggtctactcggtggcgaggcgtggtcttcgtggcataaggaagactctattcatgtaggagtacgctgcatcgagatgctcattgagtcaaccggaatggttagcttacaccgccaaaatgctggcgtagtaggtcaagactctgagactatcgaactcgcacctgaatacgctgaggctatcgcaacccgtgcaggtgcgctggctggcatctctccgatgttccaaccttgcgtagttcctcctaagccgtggactggcattactggtggtggctattgggctaacggtcgtcgtcctctggcgctggtgcgtactcacagtaagaaagcactgatgcgctacgaagacgtttacatgcctgaggtgtacaaagcgattaacattgcgcaaaacaccgcatggaaaatcaacaagaaagtcctagcggtcgccaacgtaatcaccaagtggaagcattgtccggtcgaggacatccctgcgattgagcgtgaagaactcccgatgaaaccggaagacatcgacatgaatcctgaggctctcaccgcgtggaaacgtgctgccgctgctgtgtaccgcaaggacaaggctcgcaagtctcgccgtatcagccttgagttcatgcttgagcaagccaataagtttgctaaccataaggccatctggttcccttacaacatggactggcgcggtcgtgtttacgctgtgtcaatgttcaacccgcaaggtaacgatatgaccaaaggactgcttacgctggcgaaaggtaaaccaatcggtaaggaaggttactactggctgaaaatccacggtgcaaactgtgcgggtgtcgataaggttccgttccctgagcgcatcaagttcattgaggaaaaccacgagaacatcatggcttgcgctaagtctccactggagaacacttggtgggctgagcaagattctccgttctgcttccttgcgttctgctttgagtacgctggggtacagcaccacggcctgagctataactgctcccttccgctggcgtttgacgggtcttgctctggcatccagcacttctccgcgatgctccgagatgaggtaggtggtcgcgcggttaacttgcttcctagtgaaaccgttcaggacatctacgggattgttgctaagaaagtcaacgagattctacaagcagacgcaatcaatgggaccgataacgaagtagttaccgtgaccgatgagaacactggtgaaatctctgag…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23032_sequence/1
type: Sequence
persistent identity: BBa J23032 sequence
display id: BBa_J23032_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aactagaatcacctcttgcttttgggtaagacagaagaggaga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23109_sequence/1
type: Sequence
persistent identity: BBa J23109 sequence
display id: BBa_J23109_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttacagctagctcagtcctagggactgtgctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K145014_sequence/1
type: Sequence
persistent identity: BBa K145014 sequence
display id: BBa_K145014_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgttgtttatcaagcctgcggatctccgcgaaattgtgacttttccgctatttagcgatcttgttcagtgtggctttccttcaccgatgaacacgattaacatcgctaagaacgacttctctgacatcgaactggctgctatcccgttcaacactctggctgaccattacggtgagcgtttagctcgcgaacagttggcccttgagcatgagtcttacgagatgggtgaagcacgcttccgcaagatgtttgagcgtcaacttaaagctggtgaggttgcggataacgctgccgccaagcctctcatcactaccctactccctaagatgattgcacgcatcaacgactggtttgaggaagtgaaagctaagcgcggcaagcgcccgacagccttccagttcctgcaagaaatcaagccggaagccgtagcgtacatcaccattaagaccactctggcttgcctaaccagtgctgacaatacaaccgttcaggctgtagcaagcgcaatcggtcgggccattgaggacgaggctcgcttcggtcgtatccgtgaccttgaagctaagcacttcaagaaaaacgttgaggaacaactcaacaagcgcgtagggcacgtctacaagaaagcatttatgcaagttgtcgaggctgacatgctctctaagggtctactcggtggcgaggcgtggtcttcgtggcataaggaagactctattcatgtaggagtacgctgcatcgagatgctcattgagtcaaccggaatggttagcttacaccgccaaaatgctggcgtagtaggtcaagactctgagactatcgaactcgcacctgaatacgctgaggctatcgcaacccgtgcaggtgcgctggctggcatctctccgatgttccaaccttgcgtagttcctcctaagccgtggactggcattactggtggtggctattgggctaacggtcgtcgtcctctggcgctggtgcgtactcacagtaagaaagcactgatgcgctacgaagacgtttacatgcctgaggtgtacaaagcgattaacattgcgcaaaacaccgcatggaaaatcaacaagaaagtcctagcggtcgccaacgtaatcaccaagtggaagcattgtccggtcgaggacatccctgcgattgagcgtgaagaactcccgatgaaaccggaagacatcgacatgaatcctgaggctctcaccgcgtggaaacgtgctgccgctgctgtgtaccgcaaggacaaggctcgcaagtctcgccgtatcagccttgagttcatgcttgagcaagccaataagtttgctaaccataaggccatctggttcccttacaacatggactggcgcggtcgtgtttacgctgtgtcaatgttcaacccgcaaggtaacgatatgaccaaaggactgcttacgctggcgaaaggtaaaccaatcggtaaggaaggttactactggctgaaaatccacggtgcaaactgtgcgggtgtcgataaggttccgttccctgagcgcatcaagttcattgaggaaaaccacgagaacatcatggcttgcgctaagtctccactggagaacacttggtgggctgagcaagattctccgttctgcttccttgcgttctgctttgagtacgctggggtacagcaccacggcctgagctataactgctcccttccgctggcgtttgacgggtcttgctctggcatccagcacttctccgcgatgctccgagatgaggtaggtggtcgcgcggttaacttgcttcctagtgaaaccgttcaggacatctacgggattgttgctaagaaagtcaacgagattctacaagcagacgcaatcaatgggaccgataacgaagtagttaccgtgaccgatgagaacactggtgaaatctctgagaaagtcaagctgggcactaaggcactggctggtcaatggctggcttacggtgttactcgcagtgtgactaagcgttcagtcatgacgctggc…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z