FILTER This device was designed to filter out noise in an input signal; in our case p(Tet) PoPs. If there is a short increase in transcription from this promoter, a small amount of J23009 RiboKey is produced. This is then used to unlock (J23032) the short lived UmuD-tagged T7 RNA polymerase. This results in an increase of active T7 protein. The final part of this filter is an AND gate which is used in the components further on in the system. This AND gate consists of a T7 RNA polymerase promoter with another RiboLock (J23032 again). The AND gate will only function if the duration of the noise is sufficiently long and intense (= a signal) in order to have both the short-lived RiboKey AND T7 polymerase available at the same time and thus to produce the component.
Ribokey present in higher copy number? In the new system, the lock and key have been replaced by more efficient ones
T7 with UmuD derived tag under constitutive promotor + RiboLock3d
T7 with an LVA tag under control of a constitutive promotor and a RiboLock3d
promotor weak enough? We took medium strength
Transcriptional terminator consisting of a 64 bp stem-loop.
Extension of overlapping oligonucleotides
Part composed of the T7 RNA Polymerase and the N-terminal degradation tag, but with no scar (no stop codon)
T7 RNA Polymerase with N-terminal UmuD degradation tag.
This part is different from the simple composition of the tag and the polymerase, as there cannot be a stop codon in the scar.
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Transcription terminator for the E.coli RNA polymerase.
Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
Conversion of the iGEM parts registry to SBOL2.1