##gff-version 3
##sequence-region BBa_K145254 1 862
BBa_K145254	.	terminator	734	862	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K145254	.	terminator	822	862	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K145254	.	polyA_site	849	862	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K145254	.	engineered_region	822	862	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K145254	.	stem_loop	829	848	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K145254	.	stop_codon	855	855	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K145254	.	terminator	734	813	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K145254	.	stem_loop	745	788	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K145254	.	engineered_region	734	813	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K145254	.	promoter	1	54	.	+	0	ID=BBa_R0011;Name=BBa_R0011
BBa_K145254	.	promoter	20	25	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_K145254	.	promoter	43	48	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_K145254	.	engineered_region	1	54	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_K145254	.	operator	26	42	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_K145254	.	operator	3	19	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_K145254	.	CDS	83	700	.	+	0	ID=BBa_C0061;Name=BBa_C0061
BBa_K145254	.	CDS	83	661	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_K145254	.	engineered_region	83	700	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_K145254	.	tag	662	693	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_K145254	.	engineered_tag	701	725	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_K145254	.	ribosome_entry_site	64	76	.	+	0	ID=BBa_B0032;Name=BBa_B0032
BBa_K145254	.	engineered_region	64	76	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K145254	.	ribosome_entry_site	64	76	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K145254	.	ribosome_entry_site	70	73	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
>BBa_K145254
aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagtcacacaggaaagtac
tagatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgtt
atcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaa
tgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattat
atgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgtt
ttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataa
acacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaa
gttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctatta
atgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtg
ctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatct
gttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata